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# Assembly guide of the PlanktoScope
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## Content of the Kit
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It is important to ensure that you have all of the necessary components before beginning the assembly of your PlanktoScope. To do so, please check that all bags are present as part of the kit.
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| **Bag** | **Content** |
| ------- | ---------------------------------- |
| **A** | Scews |
| **B** | Tools |
| **C** | Adhesive Pads |
| **D** | Tubing, Glass Cuvettes |
| **E** | Bubbler Pump |
| **F** | Peristaltic Pump |
| **G** | Linear Stepper Motor |
| **H** | Raspberry PI Chip cooler |
| **I** | Raspberry HAT |
| **J** | Camera Lens |
| **K** | MicroSD Card, DC Power Jack Socket |
| **L** | DC Power Supply and Cable |
| **M** | Syringe and Falcon Tube |
| **X1** | Raspberry PI 4 |
| **X2** | Pipet |
| **X3** | Cable ties |
| **X4** | Raspberry PI HQ Camera Modul |
| **X5** | Sandpaper |
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If any bags are missing, please go back to the BOM (Bill of Materials) and reorder the required components.
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## About this document
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To read this document, follow these guidelines:
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### Color codes
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- 🔴
- 🟠
- 🟡
- 🟢
- 🔵
- 🟣
### Icons
- 👁
- ⚠
- 📜
- 🎬
- ❌
As you read through the document, be sure to pay attention to these visual cues to guide you through the build process.
## Requirements
### Tools
Content of **Bag B** :
- 🔴 **B1.** Small flat screwdriver 2mm
- 🟠 **B2.** Razor blade
- 🟡 **B3.** Allen key 2mm
- 🟢 **B4.** Wrenches for standoffs
### Components
Content of **Bag A** :
- 🟢 **A1.** Standoff M2.5 - 6mm - Brass
- 🔵 **A2.** Standoff M2.5 - 15mm -
- 🟣 **A3.** Standoff M2.5 - 16mm - SS
- 🟠 **A4.** Screw M2.5x5mm CHC - SS
- 🟡 **A5.** Screw M2.5x10mm CHC - SS
- 🔴 **A6.** Screw M3x12mm BHC - SS
---
## Chapter 1: Detach the **Parts** from **panels** by cutting the **tabs**
👁 Locate the **panel S1** and discover the 5 differents **Parts F, P, K, J** and **I** .
---
🎬 Flip your **panel S1** .
---
🔴 Locate the outer **tabs** on the edges of the different **Parts** . These are typically small projections of material that are used to secure the case parts to the panels.
---
Gather all the necessary tools. You will need the B2 🟠 **Razor blade** to cut the tabs.
---
- 🟣 Use the **razor blade** to cut the outer tabs located on the edges of the different Parts
- ❌ Do not cut the inner tabs present inside the different Parts for now and focus on the outer tabs attaching the Parts to the main panel.
---
- Position your **razor blade** on the tab as close to the piece as possible to avoid residual tab after cutting.
- Press firmly on the razor blade, being very careful with your finger, to cut your first tab.
- Make sure you don't damage your table by placing a flat, rigid support under the **S1 panel** .
- Keep going with the other tabs of this piece F.
Once all of the tabs are cut, gently lift the case parts away from the panels. If the case parts are stuck or difficult to remove, you may need to gently wiggle or pry them loose using a flat tool such as a screwdriver.
!!! warning
Be extremely careful because this is very sharp.
---
Once you have removed your **Part** from the main panel by cutting off all the tabs holding it, inspect it for potential residual tabs.
- 🟣 Here is a **residual tab** that will need to be removed.
- 🟠 Here there is no **residual tab** which is perfect.
---
🟣 Place your razor **blade flat** on the edge of your piece being very careful with your fingers and cut the residual tab.
!!! warning
Be extremely careful because this is very sharp.
---
Repeat the cutting of the **tabs** on all the **Parts F, P, K, J and I** present on the **panel S1** .
!!! warning
Be extremely careful because this is very sharp.
---
🔴 Locate the **inner tabs** on the edges of the different **Parts** .
---
Cut out the **tabs** inside of all the **Parts F, P, K, J and I** detached from the **panel S1** .
Dispose of the cut tabs and any other debris that may have been created during the detachment process.
!!! warning
Be extremely careful because this is very sharp.
---
- ✅ Good way of cutting inner tabs
- ❌ Wrong way of cutting inner tabs
---
Repeat the process on the **panel S2** .
!!! warning
Be extremely careful because this is very sharp.
---
Discover the 11 differents **Parts** .
---
Dispose of the cut tabs and any other debris that may have been created during the detachment process.
Inspect the case parts and panels for any damage or imperfections that may have occurred during the detachment process. If any damage is found, it may be necessary to repair or replace the affected parts.
---
## Chapter 2: Place the 4 **Adhesive Pads** under the **Part I**
To secure the PlanktoScope on slippery grounds using the adhesive pads, follow these steps. Gather all the necessary materials. You will need:
- 👁 and Take the Part I
- 🟠 Take the four adhesive pads present in the bag A.
- 🟣 Locate the four pockets that will receive the four adhesive pads.
---
- Clean the bottom of the case part I. Make sure the surface is free of dirt, debris, and any other substances that may prevent the adhesive pads from sticking properly.
- Remove the paper and place the four adhesive pads in the pockets by pressing firmly on them, sticky-side down.
- Test the stability of the PlanktoScope by gently shaking or tilting it. If it feels secure and does not slip or slide, the adhesive pads have been successfully installed.
!!! note
🎬 Store this assembly for later.
---
## Chapter 3: Screw the four **Standoffs** into **Part A**
Now it's time to assemble the ground plate for the Raspberry Pi as the PlanktoScope main processing unit.
---
- 👁 Grab the **Part A** .
- 🟣 Locate the **four holes** on **Part A** .
---
🟢 **A1. Standoff M2.5 - 6mm** - Brass
---
🟢 **B4** . Wrenches for standoffs
---
- 🟣 Place the Standoff M2.5 - 6mm in the small side of the wrenches for
standoffs B4.
- 🟠 Do not use the big side of the wrenches for standoffs since the standoff will be loose in it.
---
- Place the standoff in the hole and start rotating **by hand** in a clockwise direction until secure.
- Then tighten with the wrench.
---
- ✅ Make sure to screw until the standoff is properly inserted in the hole.
- ❌ Do not stop screwing before.
---
Keep going for each of the **four holes** .
---
## Chapter 4: Mount the **Heat Sinks** on the **Raspberry Pi**
Locate the **Raspberry Pi 4 Model B** packaging.
!!! warning
Be careful removing it from its packaging.
---
Place the **four Heat Sinks** next to your **Raspberry Pi** and mark the locations of the **Heat Sinks** on the **Raspberry Pi** .
- 🟠 & 🔵 **Small Heat Sinks**
- 🟢 **Medium Heat Sink**
- 🟣 **Big Heat Sink**
---
Remove the protective labels under a **Heat Sink** and place the **Heat Sink** on the slot of the **Raspberry Pi** .
---
Remove the protective labels under all the **Heat Sinks** and place all the **Heat Sinks** on the slots of the **Raspberry Pi** .
---
## Chapter 5: Insert the **micro SD card** in the **Raspberry Pi**
- Locate the **SD card adapter** in the **bag K** .
- The **micro SD card** is inserted in the SD card adapter.
- 🟣 Remove the **micro SD card** from the **SD card adapter** .
---
- Flip your **Raspberry Pi** .
- 🟠 Locate the **micro SD port** .
- 🟣 Insert the **micro SD card** in the **Raspberry Pi** .
---
Push the **micro SD card** in the **Raspberry Pi** port to a point of resistance.
!!! note
If you notice that the **micro SD card** protrudes about 2mm from its slot, this is normal.
---
## Chapter 6: Mount the **Raspberry Pi** on the **Part A**
- ✅ Make sure to position the Raspberry Pi properly on the four standoffs screwed on the Part A.
- ❌ Do not invert the position of the Raspberry Pi on the four standoffs screwed on the Part A.
---
🟣 **A3. Standoff M2.5 - 16mm** - SS
---
Screw by hand a Standoff M2.5 - 16mm on the **Raspberry Pi** .
---
- Screw by hand all Standoffs M2.5 - 16mm on the **Raspberry Pi** .
- Make sure you insert all four standoffs by hand and tighten slightly.
---
🟢 **B4** . Wrenches for standoffs
---
- 🟠 Secure the Standoff M2.5 - 16 mm - SS **A3** in the big side of the wrenches for standoffs **B4** .
- 🟣 Do not use the small side of the wrenches for standoffs since the standoff won’
---
## Chapter 7: Attach the **Ribbon Cable** to the **Raspberry Pi**
Locate the **Raspberry Pi Camera HQ** packaging.
!!! warning
Be careful removing it from its packaging.
---
Lay your **Raspberry Pi Camera** face down on a suitable surface.
🔴 The **black connector** is simply a push/pull fit. To disengage the cable, pull the two corners of the black connector down, away from the camera board. It will unclip to about 3mm, make sure you don't pull it off! If you're struggling, try pulling off one corner of the connector at a time.
!!! warning
Be careful with this, this part is delicate. Lift the black connector gently
---
Once the connector has been disengaged from the Raspberry Pi camera board, the cable will simply slide out!
- 🟣 Put aside Camera the Raspberry Pi
- 🟢 Keep the Ribbon Cable for next step.
---
🔴 Locate the **black connector** present on the **Raspberry Pi** .
---
🔴 The **black connector** is simply a push/pull fit. To disengage the cable, pull the two corners of the black connector down, away from the camera board. It will unclip to about 3mm, make sure you don't pull it off! If you're struggling, try pulling off one
corner of the connector at a time.
!!! warning
Be careful, this part is delicate. Gently prise the black connector with nail or fingertip and thumb.
---
Insert the **Ribbon Cable** you just detached from the **Raspberry Pi Camera** in the **Raspberry Pi** .
- Make sure to insert in as much as you can.
- Blue rectangle on **Ribbon Cable** should face the same direction as the arrow below.
---
🔴 Secure the Ribbon Cable in the Raspberry Pi by pressing firmly on the black connector.
---
## Chapter 8: Mount the PlanktoScope HAT on the Raspberry Pi
Locate the **PlanktoScope HAT** present in **bag I** .
---
🔴 Thread the **Ribbon cable** through the **PlanktoScope HAT** slot from the
underside.
!!! warning
Make sure the two 🟣 black connectors are aligned before threading through the ribbon.
---
🔴 Plug the **PlanktoScope HAT** into the **Raspberry Pi** .
!!! warning
Make sure the two black connectors are aligned before attaching them together.
---
Press the **PlanktoScope HAT** against the **Raspberry Pi** until it is no longer possible to move them closer together.
!!! warning
Continue to feed through the Ribbon Cable and do not crush it while pressing the PlanktoScope HAT against the standoffs.
---
🟠 **A4** . Screw **M2.5X5mm** CHC - SS
---
🟣 Locate the 4 holes on the top of the **PlanktoScope HAT** and insert the four **M2.5X5mm**
---
🟡 **B3** . Allen key 2mm
---
Screw the four **A4** screws through the **PlanktoScope HAT** onto the **Standoff M2.5 - 16mm** .
!!! note
🎬 Store this assembly for later.
## Chapter 9: Place the **Power Socket** on **Part M**
- Locate the **DC Power Jack** from the **Bag K** .
- Remove the **Lock Ring** from the **DC Power Jack**
---
---
- 🔴 Lay the **Part M** down and make sure the pockets in these holes are facing upwards.
- 🟣 Locate the **Power Socket** hole on **Part M** .
---
🔴 Insert the cable inside of the hole by being sure of the orientation of the **Part M** .
---
🟣 Flip the **Part M** and secure the **DC Power Jack** by hand on the **Part M** by screwing the **Lock Ring** .
!!! warning
Make sure the Lock Ring doesn’
!!! note
🎬 Store this assembly for later.
## Chapter 10: Mount the **Raspberry Pi Camera HQ** on **Part B**
---
🟣 Locate the 4 holes on the top of the **Part B** .
---
🔵 **A2** . Standoff M2.5 - 15mm - Brass
---
Insert the four **Standoff M2.5 - 15mm** .
---
The result should be similar to the picture.
---
🟢 **B4** . Wrenches for standoffs
---
Using the small side of the **Standoff Wrench** , secure the **4 M2.5 - 15mm Standoffs**
---
- ✅ Make sure to screw until the **Standoff** is properly tightened into the hole.
- ❌ Do not stop screwing before.
---
Locate the **Raspberry Pi Camera HQ**
---
Remove the lens cap **Raspberry Pi Camera HQ** .
!!! warning
Make sure your camera lens is clean. If it is not, gently wipe using cotton swab for this task.
---
Place the **Raspberry Pi Camera HQ** on top of the four **Standoffs** installed on **Part B** .
🟣Ensure correct orientation of the **Raspberry Pi Camera HQ** . The **black connector** where the **Ribbon Cable** was removed is on the same side as the 🟢slot circled in green
---
🟠 **A4** . Screw M2.5X5mm CHC - SS
---
🟡 **B3** . Allen key 2mm
---
Use the allen key and tighten the **Raspberry Pi Camera** to the **Standoffs** .
---
The result should be similar to the picture.
!!! note
🎬 Store this assembly for later.
## Chapter 11: Mount the **Linear Stepper Motor** on **Part E**
Locate the Stepper Motors
!!! warning
Avoid touching the metal rods on the Stepper Motors
!!! info
You can touch the 🟣 gold stands
---
---
🟡 **A5** . Screw **M2.5X10mm** CHC - SS
---
🟡 **B3** . Allen key 2mm
---
- 🔴 Lay the **Part E** down and make sure the pockets in these holes are
facing upwards.
- 🟣 Locate the four holes on **Part E** and place four M2 Screws in the holes.
---
Attach the stepper motors to the screws we have just placed with the 🔴 pockets positioned on opposite to the cabling.
The result should be similar to the picture.
---
Use the 2mm allen key to fix the **Stepper Motors** .
The result should be similar to that picture.
---
The result should be similar to the picture.
---
Repeat the process on the other side with the other **Stepper Motor** .
---
Repeat the process on the other side with the other Stepper Motor.
---
The result should be similar to the picture.
!!! note
🎬 Store this assembly for later.
---
## Chapter 12: Mount the
- Locate the **LED** **LED cable** **Bag K** .
- 🟣
---
Insert the **LED** into the **LED cable** .
---
The result should be similar to the
---
---
Locate part **G** .
---
🟣 **Part G** .
---
We **G** .
---
!!! warning
Gently push the LED into the LED
---
The result should be similar to the
!!! info
🎬
---
## Chapter 13: Mount the
- Locate the **Kamoer Peristaltic pump** **Bag F** .
- 🟣 **tubing** contained in
---
---
🟢 **Peristaltic Pump** **Part O**
!!! warning
👁 Ensure the correct orientation of
---
🟡 **A5.** Screw **M2.5X10mm**
---
🟡 **B3.** Allen key 2mm **B3**
---
🟢 **M2.5X10mm**
---
Screw the two **M2.5X10mm**
---
---
🔴 **Part
---
- Place **Peristaltic** **Pump** underneath **part L** , ensuring the
- 🔴 Insert the **Peristaltic Pump** into **Part L** .
---
Insert the **Peristaltic Pump** **Part L** .
---
Insert the **Peristaltic Pump** **Part L** .
---
- Lay the assembly down.
- 🔴 **four different holes.**
---
🟡 **A5.**Screw
---
🟡 **B3.**Allen key 2mm
---
Screw the four **M2.5X10mm** in the **Part **Part L Peristaltic Pump.**
---
The result should be similar to the
!!! info
We will use this part in the next step.
---
## Chapter 14: Spiral wrap the LED and Peristaltic Pump cabling
- Locate the LED and housing, along
- 🟣 **spiral wrap** **bag K** .
---
- Spiral wrap both sets of cables
- There should be 4 cm (1.5 inches)
---
Continue wrapping around the
!!! info
The result should look the same as
---
!!! info
The result should look the same as
!!! note
🎬
---
## Chapter 15: Attaching the
Locate the **Stepper Motors** with **Raspberry Pi .
---
Feed the **Stepper Motor** **cables** into **Raspberry .
!!! warning
Make sure the orientation is
---
Feed the **Stepper Motor** **cables** into **Raspberry .
!!! warning
Make sure the orientation is
Then
---
Feed the **Stepper Motor** **cables** into **Raspberry .
!!! warning
Make sure the orientation is
Then
!!! note
The
---
## Chapter 16: Connecting the
- We will need the **Raspberry Pi HAT** with housing and Ribbon Cable along
with the **Raspberry Pi Camera** with
- We will be connecting the **Raspberry Pi Camera**
---
Gently feed the **Ribbon Cable** **Camera** .
!!! warning
👁 Ensure the correct orientation of
---
🔴
---
🔴 **B1.**Small flat screwdriver 2mm
---
- Now we will plug in the **Stepper to the **HAT** .
- 🔴The cables for the **Stepper Mounts** **HAT**
!!! warning
Hold tight, a specific order is
---
Starting with the
!!! note
The result should look the same as
---
Repeat this process with the order
---
## Chapter 17: Connect the
We will now be connecting the **LED** **Peristaltic Pump** with the **Raspberry Pi** **HAT** **Camera** .
---
Place the LED housing onto the
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!!! warning
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👁 Ensure correct orientation of both
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---
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!!! info
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The result should be similar to the
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---
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Now we will plug in the **LED** and **Stepper Mount** **Raspberry Pi** .
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---
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Feed the area of cabling that is not **spiral wrap** **spiral wrap**
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---
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We will now plug the cables into the
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---
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🟣
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---
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🔴 The two wires (LED) enter on the
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---
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The result should be similar to the
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!!! note
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We will use this assembly in the next
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---
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## Chapter 18: Connect the
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- 🟢 **DC Power Jack**
- We will plug the DC Power Jack
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---
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🔴 **B1.**Small flat screwdriver 2mm
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---
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- Insert the 🔴 red cable of the **DC into the **left side** of the
- Tighten the screw above.
---
- Insert the ⚫ black cable of the **DC **right
- Tighten the screw above.
---
!!! info
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The result should be similar to the
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!!! note
We will use this assembly in the next
---
## Chapter 19: Your PlanktoScope starts to take shape
---
Locate **Part I** **Part H** .
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---
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- Slot **Part H** **Part I** .
- 🟣 **Part H**
- 🟡 **part H**
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---
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🔴 Slot the **Peristaltic Pump** **LED** .
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---
!!! note
The result should be the same as the
---
!!! warning
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👁 Ensure the correct orientation of **Peristaltic Pump** .
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---
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At the other end, slot the **DC Power **Raspberry Pi** .
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---
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Rotate so that the DC Power Jack is
We will now slot the Raspberry Pi onto
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---
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!!! info
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The result should be similar to that
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!!! note
🎬
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---
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## Chapter 20: Inserting screws
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🟣 We
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!!! note
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🎬
---
🔴 **A6.** Screw M3X12mm BHC - SS
---
🟡 **B3.** Allen key 2mm
---
!!! info
The result should be similar to the
---
We will now turn over the
---
🟣
---
!!! info
The result should be similar to the
---
Now turn the PlanktoScope on its side.
---
---
!!! warning
👁 Ensure the orientation of your
---
Slot **Part K**
!!! warning
🟢
---
Content of **Bag A** : 🔴 **A6.** Screw M3X12mm BHC - SS
---
Content of **Bag B** : 🟡 **B3.** Allen key 2mm
---
🟣 **Part K**
---
---
We will now place **Part J**
!!! warning
👁 Ensure your PlanktoScope matches
---
🟢 Place **Part K**
---
Content of **Bag A** : 🔴 **A6.** Screw M3X12mm BHC - SS
---
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Content of **Bag B** : 🟡 **B3.** Allen key 2mm
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---
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🟣
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---
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!!! info
The result should be similar to the
---
---
Content of **Bag A** : 🟡 **A5.** Screw M2.5X10mm CHC - SS
---
Place **Part . It **cover**
---
- Place the **cover**
- 🟣 The screw will enter the hole
---
Using the allen key, tighten the screw
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---
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!!! info
The
---
## Chapter 21: Insert the tubing in the
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- Orientate
- Twist off the orange top of the
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---
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You can now remove the Peristaltic
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---
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You can now remove the Peristaltic
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---
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🔴 Locate the **Tube** **Bag F**
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!!! warning
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The tips of the Tubing that are
---
Insert the first plastic arch of the **Tube**
housing.
!!! info
The result should be similar to the
---
Insert the **Rotor**
!!! info
The result should be similar to the
---
Insert the **Rotor** **Tube** **Rotor**
!!! info
The result should be similar to the
---
- Thread the **Tube** **Rotor**
- This will require stretching the **Tube**
---
!!! info
The result should be similar to the
---
Place the Peristaltic Pump housing
---
Achieve the angle shown in the
---
!!! info
The result should be similar to the
---
- 🟣
- Once complete, locate **Bag D**
---
Push the small piece of tubing from **Bag D** **Peristaltic Pump** .
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---
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Place the long piece of tubing from **Bag D** **Peristaltic Pump** .
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---
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Insert the connector from **Bag D** **small piece of .
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---
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---
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Orientate **Part P**
---
Place **Part P** over the magnets **Peristaltic Pump** .
!!! info
The result should be similar to the
---
🔴 From **Bag M** , Place the **light blue** **Test Tube** in the hole adjacent to the
---
Place the **dark blue Test Tube**
---
🟢 **dark blue** **Test Tube** . This will serve as the waste container.
---
---
- Locate Part **C**
- Locate **25 **Lock** **Ring** **Bag J** .
- 25MM is printed on the lens.
---
- Remove the plastic lens cap.
- Slot **25 into **Part C** .
!!! info
The magnets are raised on the
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!!! warning
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Try not to touch the lens
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Screw the **Lock Ring** **flat-side down** .
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---
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- Locate Part **D** .
- Locate **16 **Lock **Bag J** .
- 16MM is printed on the lens.
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---
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- Remove the plastic lens cap.
- Slot **16 into **Part .
!!! info
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The
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!!! warning
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Try not to touch the lens
Screw the **Lock Ring** **flat-side down** .
The result should be similar to that
---
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Place **Lenses** (**C** **D** )
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!!! info
The result should be similar to the
---
Place **Lenses** **C** **D** )
The orientation of your PlanktoScope **Lenses** should match the
---
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- Once **C** and **D** ) into position, adjacent to
- Part **C**
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!!! info
The result should be similar to the
---
---
!!! info
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The Fluidic Path will have a
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Please take extra precaution while handling
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!!! warning
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The Fluidic Path is very delicate.
---
Place the **Tube Clamp** over the long **Fluidic .
---
Place the white clamp over the long **Fluidic Path** .
!!! info
The result should look the same as
---
Press down on the **Tube Clamp** so
!!! info
The result should look the same as
---
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Insert **Connector** **Tube .
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---
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Make sure the tubing is over the **Connector** .
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!!! info
The result should look the same as
---
Remove the cardboard protector **Fluidic Path** .
---
!!! warning
A reminder, the glass part of the
---
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Place another **Connector** at the end
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---
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The
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---
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⚠ Gently place the **Fluidic Path** in **Part** **F** .
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!!! warning
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Do not touch the glass.
- You may have to slightly stretch the
- The
---
If you have tape available, place a **Part F** so that the tubing remains fixed in
Avoid placing tape over the glass.
!!! info
The result should be similar to the
---
To **F** where
---
!!! info
The result should be similar to the
---
- Place the **Fluidic Path** into position
- Connect the magnets to face each
---
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The result should look the same as
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---
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- 🟢 Connect the **Fluidic Path** to the **Peristaltic Pump** by twisting the two
- The
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---
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## Build complete! 💯 💫
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The next stage is to plug in our PlanktoScope
