diff --git a/scripts/mqtt_nodered.json b/scripts/mqtt_nodered.json
index a21b696..d77d728 100644
--- a/scripts/mqtt_nodered.json
+++ b/scripts/mqtt_nodered.json
@@ -1,98 +1,2464 @@
 [
     {
-        "id": "389a2c4b.cdee1c",
-        "type": "inject",
-        "z": "3b5c4e18.844442",
-        "name": "pump forward 1 3",
-        "topic": "actuator/pump",
-        "payload": "forward 10 1",
-        "payloadType": "str",
-        "repeat": "",
-        "crontab": "",
-        "once": false,
-        "onceDelay": 0.1,
-        "x": 200,
-        "y": 460,
+        "id": "259c8713.b50e28",
+        "type": "tab",
+        "label": "Main",
+        "disabled": false,
+        "info": ""
+    },
+    {
+        "id": "6d0d6fc7.d30bb",
+        "type": "ui_text_input",
+        "z": "259c8713.b50e28",
+        "name": "sample_ship",
+        "label": "Name of the ship",
+        "tooltip": "",
+        "group": "3e1ba03d.f01d8",
+        "order": 3,
+        "width": 12,
+        "height": 1,
+        "passthru": true,
+        "mode": "text",
+        "delay": 300,
+        "topic": "sample_ship",
+        "x": 1370,
+        "y": 140,
         "wires": [
             [
-                "61943522.ba3944"
+                "de9708c2.d00068"
             ]
         ]
     },
     {
-        "id": "61943522.ba3944",
-        "type": "mqtt out",
-        "z": "3b5c4e18.844442",
-        "name": "",
+        "id": "43b12fe4.c5aa3",
+        "type": "ui_dropdown",
+        "z": "259c8713.b50e28",
+        "name": "sample_sampling_gear",
+        "label": "Sampling gear",
+        "tooltip": "",
+        "place": "Select",
+        "group": "3e1ba03d.f01d8",
+        "order": 5,
+        "width": 0,
+        "height": 0,
+        "passthru": true,
+        "options": [
+            {
+                "label": "Plankton net",
+                "value": "net",
+                "type": "str"
+            },
+            {
+                "label": "Niskin bottle 12L",
+                "value": "niskin_12L",
+                "type": "str"
+            },
+            {
+                "label": "Niskin bottle 24L",
+                "value": "niskin_24L",
+                "type": "str"
+            },
+            {
+                "label": "Pass Hull",
+                "value": "pass_hull",
+                "type": "str"
+            }
+        ],
+        "payload": "",
+        "topic": "sample_sampling_gear",
+        "x": 1330,
+        "y": 220,
+        "wires": [
+            [
+                "3c6a723.145778e"
+            ]
+        ]
+    },
+    {
+        "id": "458408d7.2b3b68",
+        "type": "ui_text_input",
+        "z": "259c8713.b50e28",
+        "name": "sample_operator",
+        "label": "Name of the operator",
+        "tooltip": "",
+        "group": "3e1ba03d.f01d8",
+        "order": 4,
+        "width": 12,
+        "height": 1,
+        "passthru": true,
+        "mode": "text",
+        "delay": 300,
+        "topic": "sample_operator",
+        "x": 1350,
+        "y": 180,
+        "wires": [
+            [
+                "a2ae6c99.188f2"
+            ]
+        ]
+    },
+    {
+        "id": "a163194f.0b17c8",
+        "type": "ui_text_input",
+        "z": "259c8713.b50e28",
+        "name": "sample_project",
+        "label": "Name of the project*",
+        "tooltip": "",
+        "group": "3e1ba03d.f01d8",
+        "order": 1,
+        "width": 12,
+        "height": 1,
+        "passthru": true,
+        "mode": "text",
+        "delay": 300,
+        "topic": "sample_project",
+        "x": 1360,
+        "y": 60,
+        "wires": [
+            [
+                "1c2c7439.47115c"
+            ]
+        ]
+    },
+    {
+        "id": "914752eb.af1b",
+        "type": "ui_text_input",
+        "z": "259c8713.b50e28",
+        "name": "sample_id",
+        "label": "ID of the station*",
+        "tooltip": "",
+        "group": "3e1ba03d.f01d8",
+        "order": 2,
+        "width": 12,
+        "height": 1,
+        "passthru": true,
+        "mode": "number",
+        "delay": 300,
+        "topic": "sample_id",
+        "x": 1370,
+        "y": 100,
+        "wires": [
+            [
+                "30648de6.8ebc52"
+            ]
+        ]
+    },
+    {
+        "id": "8a735175.23927",
+        "type": "ui_date_picker",
+        "z": "259c8713.b50e28",
+        "name": "acq_date",
+        "label": "Date :",
+        "group": "52159161.72187",
+        "order": 2,
+        "width": 12,
+        "height": 1,
+        "passthru": true,
         "topic": "",
-        "qos": "",
-        "retain": "",
-        "broker": "acc679a.89c4888",
-        "x": 390,
-        "y": 500,
+        "x": 1380,
+        "y": 1141,
+        "wires": [
+            [
+                "86aa9817.45a2e8"
+            ]
+        ]
+    },
+    {
+        "id": "16d7de1c.b0c892",
+        "type": "ui_text_input",
+        "z": "259c8713.b50e28",
+        "name": "acq_time",
+        "label": "Time :",
+        "tooltip": "",
+        "group": "52159161.72187",
+        "order": 3,
+        "width": 12,
+        "height": 1,
+        "passthru": true,
+        "mode": "time",
+        "delay": 300,
+        "topic": "",
+        "x": 1380,
+        "y": 1181,
+        "wires": [
+            [
+                "9b1b260f.e4b338"
+            ]
+        ]
+    },
+    {
+        "id": "9b1b260f.e4b338",
+        "type": "function",
+        "z": "259c8713.b50e28",
+        "name": "set object_time",
+        "func": "var time = new Date(msg.payload);\n\nvar hour = time.getUTCHours();\nif (hour<10){hour = \"0\"+hour;}\nvar minute = time.getUTCMinutes();\nif (minute<10){minute = \"0\"+minute;}\n\nvar time_UTC = \"\"+hour+minute+\"00\";\nglobal.set('object_time',time_UTC);\nreturn msg;",
+        "outputs": 1,
+        "noerr": 0,
+        "x": 1544,
+        "y": 1181,
+        "wires": [
+            [
+                "829cc940.327648"
+            ]
+        ]
+    },
+    {
+        "id": "86aa9817.45a2e8",
+        "type": "function",
+        "z": "259c8713.b50e28",
+        "name": "set object_date",
+        "func": "var date = new Date(msg.payload);\n\nvar year = date.getUTCFullYear();\nvar month = date.getUTCMonth()+1;\nif (month<10){month = \"0\"+month;}\nvar day = date.getUTCDate();\nif (day<10){day = \"0\"+day;}\n\nvar date_UTC = \"\"+year+month+day;\nglobal.set('object_date',date_UTC);\n\nreturn msg;",
+        "outputs": 1,
+        "noerr": 0,
+        "x": 1544,
+        "y": 1141,
+        "wires": [
+            [
+                "c93577b9.a1e438"
+            ]
+        ]
+    },
+    {
+        "id": "e001a28.555566",
+        "type": "function",
+        "z": "259c8713.b50e28",
+        "name": "get global",
+        "func": "msg.payload={\n    \n        \"generic_nb_step\":global.get(\"generic_nb_step\"),\n        \"generic_flowrate\":global.get(\"generic_flowrate\"),\n        \"generic_manual_volume\":global.get(\"generic_manual_volume\"),\n        \"generic_sleep_before\":global.get(\"generic_sleep_before\"),\n        \"generic_volume_before\":global.get(\"generic_volume_before\"),\n        \"generic_path\":global.get(\"generic_path\"),\n        \"generic_nb_frame\":global.get(\"generic_nb_frame\"),\n        \"generic_sleep_during\":global.get(\"generic_sleep_during\"),\n        \n        \"sample_project\":global.get(\"sample_project\"),\n        \"sample_id\":global.get(\"sample_id\"),\n        \"sample_ship\":global.get(\"sample_ship\"),\n        \"sample_operator\":global.get(\"sample_operator\"),\n        \"sample_sampling_gear\":global.get(\"sample_sampling_gear\"),\n        \n        \"acq_id\":global.get(\"acq_id\"),\n        \"acq_instrument\":global.get(\"acq_instrument\"),\n        //\"acq_instrument_id\":global.get(\"acq_instrument_id\"),\n        \"acq_celltype\":global.get(\"acq_celltype\"),\n        \"acq_minimum_mesh\":global.get(\"acq_minimum_mesh\"),\n        \"acq_maximum_mesh\":global.get(\"acq_maximum_mesh\"),\n        \"acq_min_esd\":global.get(\"acq_min_esd\"),\n        \"acq_max_esd\":global.get(\"acq_max_esd\"),\n        \"acq_volume\":global.get(\"acq_volume\"),\n        \"acq_magnification\":global.get(\"magnification\"),\n        \"acq_fnumber_objective\":global.get(\"acq_fnumber_objective\"),\n        \n        \"acq_camera_name\":\"Pi Camera V2.1 - 8MP\",\n        \n        \"object_date\":global.get(\"object_date\"),\n        \"object_time\":global.get(\"object_time\"),\n        \"object_lat\":global.get(\"object_lat\"),\n        \"object_lon\":global.get(\"object_lon\"),\n        \"object_depth_min\":global.get(\"object_depth_min\"),\n        \"object_depth_max\":global.get(\"object_depth_max\"),\n        \n        \"process_pixel\":global.get(\"process_pixel\"),\n        \"process_id\":global.get(\"process_id\")\n        \n};\nreturn msg;",
+        "outputs": 1,
+        "noerr": 0,
+        "x": 1880,
+        "y": 60,
+        "wires": [
+            [
+                "76a6e6bb.a08b98"
+            ]
+        ]
+    },
+    {
+        "id": "f25890f5.f2549",
+        "type": "file",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "filename": "/home/pi/PlanktonScope/config.txt",
+        "appendNewline": true,
+        "createDir": true,
+        "overwriteFile": "true",
+        "encoding": "none",
+        "x": 2233,
+        "y": 60,
+        "wires": [
+            []
+        ]
+    },
+    {
+        "id": "f5c1aa13.3cfbf8",
+        "type": "function",
+        "z": "259c8713.b50e28",
+        "name": "set optical config",
+        "func": "global.set(msg.topic,msg.payload);\nvar acq_fnumber_objective = String(global.get(msg.topic));\n\nswitch(acq_fnumber_objective) {\n  case \"25\":\n    global.set(\"magnification\",0.6);\n    global.set(\"process_pixel\",1.86);\n    global.set(\"sug_min\",60);\n    global.set(\"sug_max\",670);\n    global.set(\"sug_flowrate\",3);\n    break;\n  case \"16\":\n    global.set(\"magnification\",0.94);\n    global.set(\"process_pixel\",1.19);\n    global.set(\"sug_min\",40);\n    global.set(\"sug_max\",430);\n    global.set(\"sug_flowrate\",2.4);\n    break;\n  case \"12\":\n    global.set(\"magnification\",1.20);\n    global.set(\"process_pixel\",0.94);\n    global.set(\"sug_min\",30);\n    global.set(\"sug_max\",340);\n    global.set(\"sug_flowrate\",1.25);\n    break;\n  case \"8\":\n    global.set(\"magnification\",1.78);\n    global.set(\"process_pixel\",0.63);\n    global.set(\"sug_min\",20);\n    global.set(\"sug_max\",230);\n    global.set(\"sug_flowrate\",0.42);\n    break;\n  case \"6\":\n    global.set(\"magnification\",2.36);\n    global.set(\"process_pixel\",0.48);\n    global.set(\"sug_min\",15);\n    global.set(\"sug_max\",170);\n    global.set(\"sug_flowrate\",0.32);\n    break;\n}\nreturn msg;",
+        "outputs": 1,
+        "noerr": 0,
+        "x": 1550,
+        "y": 1041,
+        "wires": [
+            [
+                "72e1e23b.a8a67c"
+            ]
+        ]
+    },
+    {
+        "id": "eb9181d8.25e01",
+        "type": "ui_dropdown",
+        "z": "259c8713.b50e28",
+        "name": "acq_fnumber_objective",
+        "label": "M12 Lens*",
+        "tooltip": "",
+        "place": "Select option",
+        "group": "52159161.72187",
+        "order": 7,
+        "width": 12,
+        "height": 1,
+        "passthru": true,
+        "options": [
+            {
+                "label": "f 25mm 1/2\" 5MP IR",
+                "value": 25,
+                "type": "num"
+            },
+            {
+                "label": "f 16mm 1/2.5\" 5MP IR",
+                "value": 16,
+                "type": "num"
+            },
+            {
+                "label": "f 12mm 1/2.5\" 5MP IR",
+                "value": 12,
+                "type": "num"
+            },
+            {
+                "label": "f 8mm 1/2.5\" 5MP IR",
+                "value": 8,
+                "type": "num"
+            },
+            {
+                "label": "f 6mm 1/2.5\" 5MP IR",
+                "value": 6,
+                "type": "num"
+            }
+        ],
+        "payload": "",
+        "topic": "acq_fnumber_objective",
+        "x": 1326,
+        "y": 1041,
+        "wires": [
+            [
+                "f5c1aa13.3cfbf8"
+            ]
+        ]
+    },
+    {
+        "id": "5aba848a.7a861c",
+        "type": "ui_numeric",
+        "z": "259c8713.b50e28",
+        "name": "acq_minimum_mesh",
+        "label": "Min fraction size (μm)",
+        "tooltip": "",
+        "group": "52159161.72187",
+        "order": 8,
+        "width": 12,
+        "height": 1,
+        "wrap": false,
+        "passthru": true,
+        "topic": "acq_minimum_mesh",
+        "format": "{{value}}",
+        "min": 0,
+        "max": "300",
+        "step": "10",
+        "x": 1336,
+        "y": 841,
+        "wires": [
+            [
+                "510bd72a.b42ea8"
+            ]
+        ]
+    },
+    {
+        "id": "7cea16e.c42ace8",
+        "type": "ui_numeric",
+        "z": "259c8713.b50e28",
+        "name": "acq_maximum_mesh",
+        "label": "Max fraction size (μm)",
+        "tooltip": "",
+        "group": "52159161.72187",
+        "order": 9,
+        "width": 12,
+        "height": 1,
+        "wrap": false,
+        "passthru": true,
+        "topic": "acq_maximum_mesh",
+        "format": "{{value}}",
+        "min": "200",
+        "max": "2000",
+        "step": "100",
+        "x": 1336,
+        "y": 881,
+        "wires": [
+            [
+                "a3f18257.8a55e"
+            ]
+        ]
+    },
+    {
+        "id": "6ecc9468.0271dc",
+        "type": "ui_text_input",
+        "z": "259c8713.b50e28",
+        "name": "acq_id",
+        "label": "Acquisition unique ID*",
+        "tooltip": "",
+        "group": "52159161.72187",
+        "order": 1,
+        "width": 0,
+        "height": 0,
+        "passthru": true,
+        "mode": "number",
+        "delay": 300,
+        "topic": "acq_id",
+        "x": 1386,
+        "y": 721,
+        "wires": [
+            [
+                "b26183bf.aa217"
+            ]
+        ]
+    },
+    {
+        "id": "259fd49a.160a3c",
+        "type": "ui_dropdown",
+        "z": "259c8713.b50e28",
+        "name": "acq_celltype",
+        "label": "Thickness flowcell*",
+        "tooltip": "",
+        "place": "Select option",
+        "group": "52159161.72187",
+        "order": 6,
+        "width": 12,
+        "height": 1,
+        "passthru": true,
+        "options": [
+            {
+                "label": "200 μm µ-Slide I Luer",
+                "value": 200,
+                "type": "num"
+            },
+            {
+                "label": "400 μm µ-Slide I Luer",
+                "value": 400,
+                "type": "num"
+            },
+            {
+                "label": "600 μm µ-Slide I Luer",
+                "value": 600,
+                "type": "num"
+            },
+            {
+                "label": "800 μm µ-Slide I Luer",
+                "value": 800,
+                "type": "num"
+            }
+        ],
+        "payload": "",
+        "topic": "acq_celltype",
+        "x": 1366,
+        "y": 801,
+        "wires": [
+            [
+                "d96d32ec.f88b9"
+            ]
+        ]
+    },
+    {
+        "id": "66a3425c.79aabc",
+        "type": "ui_text_input",
+        "z": "259c8713.b50e28",
+        "name": "acq_volume",
+        "label": "Volume to pass (ml)",
+        "tooltip": "",
+        "group": "52159161.72187",
+        "order": 5,
+        "width": 12,
+        "height": 1,
+        "passthru": true,
+        "mode": "number",
+        "delay": 300,
+        "topic": "acq_volume",
+        "x": 1366,
+        "y": 921,
+        "wires": [
+            [
+                "a3ec68a2.889ca8"
+            ]
+        ]
+    },
+    {
+        "id": "6722bc48.d4f354",
+        "type": "comment",
+        "z": "259c8713.b50e28",
+        "name": "sample",
+        "info": "SAMPLE: a collection event\n\n    sample_id [t] : unique identifier\n    sample_*** [f] or [t] : other fields relative to the sample\n",
+        "x": 810,
+        "y": 60,
         "wires": []
     },
     {
-        "id": "40a5838d.ad561c",
+        "id": "f93037f1.6a6238",
+        "type": "comment",
+        "z": "259c8713.b50e28",
+        "name": "acquisition",
+        "info": "ACQUISITION: metadata relative to the image acquisition\n\n    acq_id [t] : unique identifier. If your acquisition differ for each sample you must have different identifier.\n    acq_instrument [t] : name of the instrument (UVP, ZOOSCAN, FLOWCAM, etc.)\n    acq_*** [f] or [t] : other fields relative to the acquisition\n",
+        "x": 800,
+        "y": 720,
+        "wires": []
+    },
+    {
+        "id": "734ba3db.3f82fc",
+        "type": "comment",
+        "z": "259c8713.b50e28",
+        "name": "object",
+        "info": "OBJECT: one object to be classified, usually one organism. One object can be represented by several images. In this tsv file, there is one line per image which means the object data gets repeated on several lines.\n\n    object_id [t] : unique object name (will be displayed in Ecotaxa object page)\n    object_link [f] : URL of an associated website\n    object_lat [f] : latitude, decimal degrees\n    object_lon [f] : longitude, decimal degrees\n    object_date [f] : ISO8601 YYYYMMJJ UTC\n    object_time [f] : ISO8601 HHMMSS UTC\n    object_depth_min [f] : minimum depth of object, meters\n    object_depth_max [f] : maximum depth of object, meters\n\nAnd, for already classified objects\n\n    object_annotation_date [t] : ISO8601 YYYYMMJJ UTC\n    object_annotation_time [t] : ISO8601 YYYYMMJJ UTC\n    object_annotation_category [t] : class of the object with optionnaly its direct parent following separated by left angle bracket without whitespace \"Cnidaria<Hydrozoa\" or old style between brackets \"Cnidaria (Hydrozoa)\"\n    object_annotation_category_id [f] : Ecotaxa ID of the class of the object, generaly from an Ecotaxa export\n    object_annotation_person_name [t] : name of the person who identified the object\n    object_annotation_person_email [t] : email of the person who identified the object\n    object_annotation_status [t] : predicted, dubious, or validated\n",
+        "x": 810,
+        "y": 1140,
+        "wires": []
+    },
+    {
+        "id": "5866c9a4.e1ca88",
+        "type": "ui_text_input",
+        "z": "259c8713.b50e28",
+        "name": "acq_instrument",
+        "label": "Acquisition instrument",
+        "tooltip": "PlanktonScope V2.1",
+        "group": "52159161.72187",
+        "order": 4,
+        "width": 12,
+        "height": 1,
+        "passthru": true,
+        "mode": "text",
+        "delay": 300,
+        "topic": "acq_instrument",
+        "x": 1356,
+        "y": 761,
+        "wires": [
+            [
+                "1d641ab3.6ad485"
+            ]
+        ]
+    },
+    {
+        "id": "76a6e6bb.a08b98",
+        "type": "json",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "property": "payload",
+        "action": "str",
+        "pretty": true,
+        "x": 2023,
+        "y": 60,
+        "wires": [
+            [
+                "f25890f5.f2549"
+            ]
+        ]
+    },
+    {
+        "id": "594ec843.9d4458",
+        "type": "file in",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "filename": "/home/pi/PlanktonScope/config.txt",
+        "format": "utf8",
+        "chunk": false,
+        "sendError": false,
+        "encoding": "none",
+        "x": 320,
+        "y": 60,
+        "wires": [
+            [
+                "3bc1a977.4e9c76"
+            ]
+        ]
+    },
+    {
+        "id": "d9ca0e54.f8b58",
         "type": "inject",
-        "z": "3b5c4e18.844442",
-        "name": "All off",
-        "topic": "actuator/wait",
+        "z": "259c8713.b50e28",
+        "name": "on_load",
+        "topic": "",
+        "payload": "",
+        "payloadType": "date",
+        "repeat": "",
+        "crontab": "",
+        "once": true,
+        "onceDelay": 0.1,
+        "x": 100,
+        "y": 60,
+        "wires": [
+            [
+                "594ec843.9d4458",
+                "ca38a11d.0ee54"
+            ]
+        ]
+    },
+    {
+        "id": "3bc1a977.4e9c76",
+        "type": "json",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "property": "payload",
+        "action": "",
+        "pretty": false,
+        "x": 550,
+        "y": 60,
+        "wires": [
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+    {
+        "id": "2582798f.eddcc6",
+        "type": "function",
+        "z": "259c8713.b50e28",
+        "name": "get sample_projet",
+        "func": "msg.payload = msg.payload.sample_project;\nreturn msg;",
+        "outputs": 1,
+        "noerr": 0,
+        "x": 1030,
+        "y": 60,
+        "wires": [
+            [
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+            ]
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+        "id": "1590a418.9166dc",
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+        "z": "259c8713.b50e28",
+        "name": "get sample_ship",
+        "func": "msg.payload = msg.payload.sample_ship;\nreturn msg;",
+        "outputs": 1,
+        "noerr": 0,
+        "x": 1020,
+        "y": 140,
+        "wires": [
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+        "type": "function",
+        "z": "259c8713.b50e28",
+        "name": "get sample_id",
+        "func": "msg.payload = msg.payload.sample_id+1;\nreturn msg;",
+        "outputs": 1,
+        "noerr": 0,
+        "x": 1020,
+        "y": 100,
+        "wires": [
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+        "z": "259c8713.b50e28",
+        "name": "get sample_operator",
+        "func": "msg.payload = msg.payload.sample_operator;\nreturn msg;",
+        "outputs": 1,
+        "noerr": 0,
+        "x": 1040,
+        "y": 180,
+        "wires": [
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+        "type": "function",
+        "z": "259c8713.b50e28",
+        "name": "get sample_sampling_gear",
+        "func": "msg.payload = msg.payload.sample_sampling_gear;\nreturn msg;",
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+        "noerr": 0,
+        "x": 1060,
+        "y": 220,
+        "wires": [
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+        "z": "259c8713.b50e28",
+        "name": "get acq_id",
+        "func": "msg.payload = msg.payload.acq_id+1;\nreturn msg;",
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+        "noerr": 0,
+        "x": 1010,
+        "y": 721,
+        "wires": [
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+        "z": "259c8713.b50e28",
+        "name": "get acq_instrument",
+        "func": "msg.payload = msg.payload.acq_instrument;\nreturn msg;",
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+        "noerr": 0,
+        "x": 1030,
+        "y": 761,
+        "wires": [
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+        "z": "259c8713.b50e28",
+        "name": "get acq_celltype",
+        "func": "msg.payload = msg.payload.acq_celltype;\nreturn msg;",
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+        "noerr": 0,
+        "x": 1020,
+        "y": 801,
+        "wires": [
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+        "name": "get acq_minimum_mesh",
+        "func": "msg.payload = msg.payload.acq_minimum_mesh;\nreturn msg;",
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+        "noerr": 0,
+        "x": 1050,
+        "y": 841,
+        "wires": [
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+        "name": "get acq_maximum_mesh",
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+        "x": 1050,
+        "y": 881,
+        "wires": [
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+        "z": "259c8713.b50e28",
+        "name": "get acq_volume",
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+        "x": 1020,
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+        "z": "259c8713.b50e28",
+        "name": "get date_now",
+        "func": "msg.payload = Date.now();\nreturn msg;",
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+        "noerr": 0,
+        "x": 1024,
+        "y": 1141,
+        "wires": [
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+        "z": "259c8713.b50e28",
+        "name": "get time_now",
+        "func": "msg.payload = Date.now()\nreturn msg;",
+        "outputs": 1,
+        "noerr": 0,
+        "x": 1014,
+        "y": 1181,
+        "wires": [
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+        "z": "259c8713.b50e28",
+        "name": "get acq_fnumber_objective",
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+        "x": 1060,
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+        "group": "52159161.72187",
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+        "height": 1,
+        "wrap": false,
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+        "height": 1,
+        "wrap": false,
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+        "x": 1740,
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+        "x": 1044,
+        "y": 1303,
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+        "name": "process_id",
+        "label": "Id of the process",
+        "tooltip": "",
+        "group": "8cfadfab.327d7",
+        "order": 6,
+        "width": 12,
+        "height": 1,
+        "passthru": true,
+        "mode": "number",
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+        "type": "comment",
+        "z": "259c8713.b50e28",
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+        "info": "PROCESS: metadata relative to the processing of the raw images\n\n    process_id [t] : unique identifier. If your processing differ for each sample you must have different identifier.\n    process_*** [f] or [t] : other fields relative to the process\n",
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+            "72e1e23b.a8a67c",
+            "c93577b9.a1e438",
+            "829cc940.327648",
+            "3d141631.d548ba",
+            "644e95b9.04b7ac",
+            "d1b907a3.6f56b8",
+            "3cd0a17b.0cc42e",
+            "5ea01613.8cc9d8",
+            "61382d0b.60c534"
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+        "x": 1635,
+        "y": 60,
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+        "type": "link out",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "links": [
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+        "x": 1475,
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+        "wires": []
+    },
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+        "links": [
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+        "x": 1475,
+        "y": 761,
+        "wires": []
+    },
+    {
+        "id": "d96d32ec.f88b9",
+        "type": "link out",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "links": [
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+        "wires": []
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+        "name": "",
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+        "name": "",
+        "links": [
+            "7532db82.1dcf04"
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+        "x": 1475,
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+    },
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+        "wires": []
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+        "type": "link out",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "links": [
+            "7532db82.1dcf04"
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+        "x": 1475,
+        "y": 60,
+        "wires": []
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+        "type": "link out",
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+        "name": "",
+        "links": [
+            "7532db82.1dcf04"
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+        "x": 1475,
+        "y": 140,
+        "wires": []
+    },
+    {
+        "id": "30648de6.8ebc52",
+        "type": "link out",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "links": [
+            "7532db82.1dcf04"
+        ],
+        "x": 1475,
+        "y": 100,
+        "wires": []
+    },
+    {
+        "id": "a2ae6c99.188f2",
+        "type": "link out",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "links": [
+            "7532db82.1dcf04"
+        ],
+        "x": 1475,
+        "y": 180,
+        "wires": []
+    },
+    {
+        "id": "3c6a723.145778e",
+        "type": "link out",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "links": [
+            "7532db82.1dcf04"
+        ],
+        "x": 1475,
+        "y": 220,
+        "wires": []
+    },
+    {
+        "id": "767b43a1.de21dc",
+        "type": "ui_worldmap",
+        "z": "259c8713.b50e28",
+        "d": true,
+        "group": "cef1e703.bcf3c8",
+        "order": 1,
+        "width": 0,
+        "height": 0,
+        "name": "",
+        "lat": "1.5",
+        "lon": "1.5",
+        "zoom": "4",
+        "layer": "OSM grey",
+        "cluster": "1",
+        "maxage": "",
+        "usermenu": "hide",
+        "layers": "hide",
+        "panit": "false",
+        "panlock": "false",
+        "zoomlock": "false",
+        "hiderightclick": "true",
+        "coords": "deg",
+        "showgrid": "false",
+        "path": "/worldmap",
+        "x": 1380,
+        "y": 1221,
+        "wires": []
+    },
+    {
+        "id": "2327c165.8397ae",
+        "type": "function",
+        "z": "259c8713.b50e28",
+        "name": "get object_lat & object_lon",
+        "func": "\nreturn msg;",
+        "outputs": 1,
+        "noerr": 0,
+        "x": 1060,
+        "y": 1221,
+        "wires": [
+            [
+                "767b43a1.de21dc"
+            ]
+        ]
+    },
+    {
+        "id": "fa47061e.34a218",
+        "type": "ui_numeric",
+        "z": "259c8713.b50e28",
+        "name": "acq_min_esd",
+        "label": "Minimum size to segment (μm)",
+        "tooltip": "",
+        "group": "52159161.72187",
+        "order": 10,
+        "width": 12,
+        "height": 1,
+        "wrap": false,
+        "passthru": true,
+        "topic": "acq_min_esd",
+        "format": "{{value}}",
+        "min": 0,
+        "max": "300",
+        "step": "5",
+        "x": 1366,
+        "y": 961,
+        "wires": [
+            [
+                "2c95d05e.4668b"
+            ]
+        ]
+    },
+    {
+        "id": "6f36bd6d.154d14",
+        "type": "ui_numeric",
+        "z": "259c8713.b50e28",
+        "name": "acq_max_esd",
+        "label": "Maximum size to segment (μm)",
+        "tooltip": "",
+        "group": "52159161.72187",
+        "order": 11,
+        "width": 12,
+        "height": 1,
+        "wrap": false,
+        "passthru": true,
+        "topic": "acq_max_esd",
+        "format": "{{value}}",
+        "min": "100",
+        "max": "2000",
+        "step": "5",
+        "x": 1356,
+        "y": 1001,
+        "wires": [
+            [
+                "8c499d09.7a2a"
+            ]
+        ]
+    },
+    {
+        "id": "277b6764.edb1f8",
+        "type": "function",
+        "z": "259c8713.b50e28",
+        "name": "get acq_min_esd",
+        "func": "msg.payload = msg.payload.acq_min_esd;\nreturn msg;",
+        "outputs": 1,
+        "noerr": 0,
+        "x": 1030,
+        "y": 961,
+        "wires": [
+            [
+                "fa47061e.34a218"
+            ]
+        ]
+    },
+    {
+        "id": "75b10c7c.8433a4",
+        "type": "function",
+        "z": "259c8713.b50e28",
+        "name": "get acq_max_esd",
+        "func": "msg.payload = msg.payload.acq_max_esd;\nreturn msg;",
+        "outputs": 1,
+        "noerr": 0,
+        "x": 1030,
+        "y": 1001,
+        "wires": [
+            [
+                "6f36bd6d.154d14"
+            ]
+        ]
+    },
+    {
+        "id": "2673deea.4cc532",
+        "type": "link in",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "links": [
+            "b368cae7.dd66b8"
+        ],
+        "x": 895,
+        "y": 961,
+        "wires": [
+            [
+                "277b6764.edb1f8"
+            ]
+        ]
+    },
+    {
+        "id": "245f70f0.16b7b",
+        "type": "link in",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "links": [
+            "b368cae7.dd66b8"
+        ],
+        "x": 895,
+        "y": 1001,
+        "wires": [
+            [
+                "75b10c7c.8433a4"
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+    },
+    {
+        "id": "2c95d05e.4668b",
+        "type": "link out",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "links": [
+            "7532db82.1dcf04"
+        ],
+        "x": 1475,
+        "y": 961,
+        "wires": []
+    },
+    {
+        "id": "8c499d09.7a2a",
+        "type": "link out",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "links": [
+            "7532db82.1dcf04"
+        ],
+        "x": 1475,
+        "y": 1001,
+        "wires": []
+    },
+    {
+        "id": "5f4a72d1.9f6adc",
+        "type": "ui_text_input",
+        "z": "259c8713.b50e28",
+        "name": "nb_step",
+        "label": "Number of step(s)",
+        "tooltip": "",
+        "group": "fbd92986.1028c8",
+        "order": 3,
+        "width": 8,
+        "height": 1,
+        "passthru": true,
+        "mode": "number",
+        "delay": 300,
+        "topic": "nb_step",
+        "x": 1380,
+        "y": 341,
+        "wires": [
+            [
+                "299221dd.e8f85e"
+            ]
+        ]
+    },
+    {
+        "id": "89474615.765a38",
+        "type": "comment",
+        "z": "259c8713.b50e28",
+        "name": "Focus settings",
+        "info": "ACQUISITION: metadata relative to the image acquisition\n\n    acq_id [t] : unique identifier. If your acquisition differ for each sample you must have different identifier.\n    acq_instrument [t] : name of the instrument (UVP, ZOOSCAN, FLOWCAM, etc.)\n    acq_*** [f] or [t] : other fields relative to the acquisition\n",
+        "x": 790,
+        "y": 300,
+        "wires": []
+    },
+    {
+        "id": "299221dd.e8f85e",
+        "type": "link out",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "links": [
+            "7532db82.1dcf04"
+        ],
+        "x": 1475,
+        "y": 341,
+        "wires": []
+    },
+    {
+        "id": "81a7627c.bac92",
+        "type": "function",
+        "z": "259c8713.b50e28",
+        "name": "get generic_nb_step",
+        "func": "msg.payload = msg.payload.generic_nb_step;\nreturn msg;",
+        "outputs": 1,
+        "noerr": 0,
+        "x": 1040,
+        "y": 341,
+        "wires": [
+            [
+                "5f4a72d1.9f6adc"
+            ]
+        ]
+    },
+    {
+        "id": "d949ef9d.b1fa4",
+        "type": "link in",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "links": [
+            "b368cae7.dd66b8"
+        ],
+        "x": 895,
+        "y": 341,
+        "wires": [
+            [
+                "81a7627c.bac92"
+            ]
+        ]
+    },
+    {
+        "id": "705dddc5.567bc4",
+        "type": "ui_button",
+        "z": "259c8713.b50e28",
+        "name": "DOWN",
+        "group": "fbd92986.1028c8",
+        "order": 4,
+        "width": 6,
+        "height": 1,
+        "passthru": true,
+        "label": "",
+        "tooltip": "",
+        "color": "",
+        "bgcolor": "",
+        "icon": "arrow_downward",
+        "payload": "DOWN",
+        "payloadType": "str",
+        "topic": "actuator/focus",
+        "x": 1380,
+        "y": 420,
+        "wires": [
+            [
+                "4f74f017.00a58"
+            ]
+        ]
+    },
+    {
+        "id": "b0aacff7.56651",
+        "type": "ui_button",
+        "z": "259c8713.b50e28",
+        "name": "UP",
+        "group": "fbd92986.1028c8",
+        "order": 2,
+        "width": 6,
+        "height": 1,
+        "passthru": false,
+        "label": "",
+        "tooltip": "",
+        "color": "",
+        "bgcolor": "",
+        "icon": "arrow_upwards",
+        "payload": "UP",
+        "payloadType": "str",
+        "topic": "actuator/focus",
+        "x": 1390,
+        "y": 381,
+        "wires": [
+            [
+                "4f74f017.00a58"
+            ]
+        ]
+    },
+    {
+        "id": "1b9f457d.9be43b",
+        "type": "ui_text_input",
+        "z": "259c8713.b50e28",
+        "name": "manual_volume",
+        "label": "Volume to pass (ml)",
+        "tooltip": "",
+        "group": "707d9797.c8e798",
+        "order": 3,
+        "width": 8,
+        "height": 1,
+        "passthru": true,
+        "mode": "number",
+        "delay": 300,
+        "topic": "manual_volume",
+        "x": 1360,
+        "y": 541,
+        "wires": [
+            [
+                "b2660124.01b81"
+            ]
+        ]
+    },
+    {
+        "id": "b2660124.01b81",
+        "type": "link out",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "links": [
+            "7532db82.1dcf04"
+        ],
+        "x": 1475,
+        "y": 541,
+        "wires": []
+    },
+    {
+        "id": "3e9c4e74.aee022",
+        "type": "function",
+        "z": "259c8713.b50e28",
+        "name": "pump.js",
+        "func": "state = global.get(\"state\");\n\nif (state == null){state=\"free\"}\n\nvar manual_volume= global.get(\"manual_volume\");\nvar flowrate= global.get(\"flowrate\");\n\nif (manual_volume === undefined || manual_volume === \"\" || manual_volume === null) {\n    msg.topic = \"Missing entry :\"\n    msg.payload = \"Volume to pass\";\n    \n}else if (flowrate === undefined || flowrate === \"\" || flowrate === null) {\n    msg.topic = \"Missing entry :\"\n    msg.payload = \"Flowrate\";\n    \n}else {\n    volume = global.get(\"manual_volume\");\n    nb_step=volume*507\n    msg.volume = volume;\n    flowrate = global.get(\"flowrate\");\n    duration=(volume*60)/flowrate\n    delay=(duration/nb_step)-0.005\n    msg.topic = \"actuator/pump\";\n    \n    if(msg.payload === \"FORWARD\" & state===\"free\"){\n        msg.payload='FORWARD '+delay+' '+nb_step;\n    }\n    if(msg.payload === \"BACKWARD\" & state===\"free\"){\n        msg.payload='BACKWARD '+delay+' '+nb_step;\n    }\n}\nreturn msg;",
+        "outputs": 1,
+        "noerr": 0,
+        "x": 1520,
+        "y": 600,
+        "wires": [
+            [
+                "98e2e9e0.fb7418"
+            ]
+        ],
+        "info": "### Focusing\n##### focus.py `nb_step` `orientation`\n\n- `nb_step` : **integer** (from 1 to 100000) - number of step to perform by the stage (about 31um/step)\n- `orientation` : **string** - orientation of the focus either `up` or `down`\n\nExample:\n\n    python3.7 $HOME/PlanktonScope/scripts/focus.py 650 up\n"
+    },
+    {
+        "id": "3f0df968.b0d286",
+        "type": "ui_button",
+        "z": "259c8713.b50e28",
+        "name": "BACKWARD",
+        "group": "707d9797.c8e798",
+        "order": 2,
+        "width": 6,
+        "height": 1,
+        "passthru": false,
+        "label": "",
+        "tooltip": "",
+        "color": "",
+        "bgcolor": "",
+        "icon": "arrow_back",
+        "payload": "BACKWARD",
+        "payloadType": "str",
+        "topic": "actuator/pump",
+        "x": 1370,
+        "y": 580,
+        "wires": [
+            [
+                "3e9c4e74.aee022"
+            ]
+        ]
+    },
+    {
+        "id": "214fb242.9abb6e",
+        "type": "ui_button",
+        "z": "259c8713.b50e28",
+        "name": "FORWARD",
+        "group": "707d9797.c8e798",
+        "order": 4,
+        "width": 6,
+        "height": 1,
+        "passthru": true,
+        "label": "",
+        "tooltip": "",
+        "color": "",
+        "bgcolor": "",
+        "icon": "arrow_forward",
+        "payload": "FORWARD",
+        "payloadType": "str",
+        "topic": "actuator/pump",
+        "x": 1370,
+        "y": 620,
+        "wires": [
+            [
+                "3e9c4e74.aee022"
+            ]
+        ]
+    },
+    {
+        "id": "7c38cc6e.c932e4",
+        "type": "link in",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "links": [
+            "b368cae7.dd66b8"
+        ],
+        "x": 895,
+        "y": 541,
+        "wires": [
+            [
+                "31931ba8.ab01d4"
+            ]
+        ]
+    },
+    {
+        "id": "31931ba8.ab01d4",
+        "type": "function",
+        "z": "259c8713.b50e28",
+        "name": "get generic_manual_volume",
+        "func": "msg.payload = msg.payload.generic_manual_volume;\nreturn msg;",
+        "outputs": 1,
+        "noerr": 0,
+        "x": 1060,
+        "y": 541,
+        "wires": [
+            [
+                "1b9f457d.9be43b"
+            ]
+        ]
+    },
+    {
+        "id": "1d58e86f.a3a9e8",
+        "type": "rpi-gpio out",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "pin": "40",
+        "set": "",
+        "level": "0",
+        "freq": "",
+        "out": "out",
+        "x": 420,
+        "y": 161,
+        "wires": []
+    },
+    {
+        "id": "3e58f066.366b5",
+        "type": "ui_template",
+        "z": "259c8713.b50e28",
+        "group": "fbd92986.1028c8",
+        "name": "Stream Pi Camera",
+        "order": 1,
+        "width": 24,
+        "height": 11,
+        "format": "<center><iframe id=\"inlineFrameExample\"\n    title=\"Inline Frame Example\"\n    height=\"600\"\n    width=\"800\"\n    frameborder=\"0\"\n    src=\"http://localhost/html/min.php\">\n</iframe></center>",
+        "storeOutMessages": true,
+        "fwdInMessages": true,
+        "templateScope": "local",
+        "x": 1210,
+        "y": 300,
+        "wires": [
+            []
+        ]
+    },
+    {
+        "id": "b7d9455c.026588",
+        "type": "switch",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "property": "topic",
+        "propertyType": "msg",
+        "rules": [
+            {
+                "t": "eq",
+                "v": "actuator/focus",
+                "vt": "str"
+            },
+            {
+                "t": "eq",
+                "v": "Missing entry :",
+                "vt": "str"
+            }
+        ],
+        "checkall": "true",
+        "repair": false,
+        "outputs": 2,
+        "x": 1670,
+        "y": 400,
+        "wires": [
+            [
+                "fdb3b132.8b341"
+            ],
+            [
+                "b9d996d6.37c2f8"
+            ]
+        ]
+    },
+    {
+        "id": "b9d996d6.37c2f8",
+        "type": "ui_toast",
+        "z": "259c8713.b50e28",
+        "position": "dialog",
+        "displayTime": "3",
+        "highlight": "",
+        "sendall": true,
+        "outputs": 1,
+        "ok": "OK",
+        "cancel": "",
+        "raw": false,
+        "topic": "",
+        "name": "",
+        "x": 1830,
+        "y": 420,
+        "wires": [
+            []
+        ]
+    },
+    {
+        "id": "ca38a11d.0ee54",
+        "type": "function",
+        "z": "259c8713.b50e28",
+        "name": "init LED",
+        "func": "msg.payload=1;\nreturn msg;",
+        "outputs": 1,
+        "noerr": 0,
+        "x": 240,
+        "y": 161,
+        "wires": [
+            [
+                "1d58e86f.a3a9e8"
+            ]
+        ]
+    },
+    {
+        "id": "75701b99.c6e974",
+        "type": "exec",
+        "z": "259c8713.b50e28",
+        "command": "vcgencmd measure_temp | tr -d \"temp=\" | tr -d \"'C\" | tr -d \"\\n\"",
+        "addpay": false,
+        "append": "",
+        "useSpawn": "",
+        "timer": "",
+        "name": "RPi Temp.",
+        "x": 2750,
+        "y": 180,
+        "wires": [
+            [
+                "aac61390.c13c6"
+            ],
+            [],
+            []
+        ]
+    },
+    {
+        "id": "1780384d.718198",
+        "type": "inject",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "topic": "",
+        "payload": "",
+        "payloadType": "date",
+        "repeat": "10",
+        "crontab": "",
+        "once": false,
+        "onceDelay": "",
+        "x": 2590,
+        "y": 180,
+        "wires": [
+            [
+                "75701b99.c6e974"
+            ]
+        ]
+    },
+    {
+        "id": "aac61390.c13c6",
+        "type": "python3-function",
+        "z": "259c8713.b50e28",
+        "name": "fan.py",
+        "func": "#!/usr/bin/python\nimport smbus\nimport sys\n\ntemp = msg[\"payload\"]\n\ntemp = int(temp.split('.',1)[0])\n\nbus = smbus.SMBus(1)\n\nDEVICE_ADDRESS = 0x0d\n\nif temp < 38:\n    bus.write_byte_data(DEVICE_ADDRESS, 0x08, 0x00)\n    bus.write_byte_data(DEVICE_ADDRESS, 0x08, 0x00)\nif temp > 42:\n    bus.write_byte_data(DEVICE_ADDRESS, 0x08, 0x01)\n    bus.write_byte_data(DEVICE_ADDRESS, 0x08, 0x01)",
+        "outputs": 1,
+        "x": 2930,
+        "y": 180,
+        "wires": [
+            []
+        ]
+    },
+    {
+        "id": "603d5fab.08f56",
+        "type": "comment",
+        "z": "259c8713.b50e28",
+        "name": "save config.txt",
+        "info": "",
+        "x": 1690,
+        "y": 20,
+        "wires": []
+    },
+    {
+        "id": "72e1e23b.a8a67c",
+        "type": "link out",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "links": [
+            "7532db82.1dcf04",
+            "d473399b.081ae8"
+        ],
+        "x": 1675,
+        "y": 1040,
+        "wires": []
+    },
+    {
+        "id": "d473399b.081ae8",
+        "type": "link in",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "links": [
+            "72e1e23b.a8a67c",
+            "c93577b9.a1e438",
+            "829cc940.327648"
+        ],
+        "x": 1635,
+        "y": 100,
+        "wires": [
+            [
+                "e001a28.555566"
+            ]
+        ]
+    },
+    {
+        "id": "c93577b9.a1e438",
+        "type": "link out",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "links": [
+            "7532db82.1dcf04",
+            "d473399b.081ae8"
+        ],
+        "x": 1675,
+        "y": 1140,
+        "wires": []
+    },
+    {
+        "id": "829cc940.327648",
+        "type": "link out",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "links": [
+            "7532db82.1dcf04",
+            "d473399b.081ae8"
+        ],
+        "x": 1675,
+        "y": 1180,
+        "wires": []
+    },
+    {
+        "id": "98e2e9e0.fb7418",
+        "type": "switch",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "property": "topic",
+        "propertyType": "msg",
+        "rules": [
+            {
+                "t": "eq",
+                "v": "actuator/pump",
+                "vt": "str"
+            },
+            {
+                "t": "eq",
+                "v": "Missing entry :",
+                "vt": "str"
+            }
+        ],
+        "checkall": "true",
+        "repair": false,
+        "outputs": 2,
+        "x": 1670,
+        "y": 600,
+        "wires": [
+            [
+                "61fb057d.3c251c"
+            ],
+            [
+                "46a536f0.c83138"
+            ]
+        ]
+    },
+    {
+        "id": "46a536f0.c83138",
+        "type": "ui_toast",
+        "z": "259c8713.b50e28",
+        "position": "dialog",
+        "displayTime": "3",
+        "highlight": "",
+        "sendall": true,
+        "outputs": 1,
+        "ok": "OK",
+        "cancel": "",
+        "raw": false,
+        "topic": "",
+        "name": "",
+        "x": 1830,
+        "y": 620,
+        "wires": [
+            []
+        ]
+    },
+    {
+        "id": "24ffcf03.2a51b",
+        "type": "ui_button",
+        "z": "259c8713.b50e28",
+        "name": "stop pump",
+        "group": "707d9797.c8e798",
+        "order": 5,
+        "width": 4,
+        "height": 1,
+        "passthru": true,
+        "label": "STOP PUMP",
+        "tooltip": "",
+        "color": "",
+        "bgcolor": "",
+        "icon": "",
         "payload": "off",
         "payloadType": "str",
-        "repeat": "",
-        "crontab": "",
-        "once": false,
-        "onceDelay": 0.1,
-        "x": 170,
-        "y": 540,
+        "topic": "actuator/wait",
+        "x": 1370,
+        "y": 660,
         "wires": [
             [
-                "61943522.ba3944"
+                "e2a7220a.09d43"
             ]
         ]
     },
     {
-        "id": "3383d89a.499038",
-        "type": "inject",
-        "z": "3b5c4e18.844442",
-        "name": "focus on",
-        "topic": "actuator/focus",
-        "payload": "up 1000",
+        "id": "7eb16c3b.294e54",
+        "type": "comment",
+        "z": "259c8713.b50e28",
+        "name": "turn ON the LED",
+        "info": "",
+        "x": 260,
+        "y": 121,
+        "wires": []
+    },
+    {
+        "id": "4f74f017.00a58",
+        "type": "function",
+        "z": "259c8713.b50e28",
+        "name": "focus.js",
+        "func": "state = global.get(\"state\");\n\nif (state == null){state=\"free\"}\n\nvar nb_step= global.get(\"nb_step\");\n\nif (nb_step === undefined || nb_step === \"\" || nb_step === null) {\n    msg.topic = \"Missing entry :\"\n    msg.payload = \"Number of steps\";\n    \n}else {\n    nb_step= global.get(\"nb_step\");\n    if(msg.payload === \"UP\" & state===\"free\"){\n        msg.payload=\"FORWARD \"+nb_step;\n    }\n    if(msg.payload === \"DOWN\" & state===\"free\"){\n        msg.payload=\"BACKWARD \"+nb_step;\n    }\n}\nreturn msg;",
+        "outputs": 1,
+        "noerr": 0,
+        "x": 1520,
+        "y": 400,
+        "wires": [
+            [
+                "b7d9455c.026588"
+            ]
+        ],
+        "info": "### Focusing\n##### focus.py `nb_step` `orientation`\n\n- `nb_step` : **integer** (from 1 to 100000) - number of step to perform by the stage (about 31um/step)\n- `orientation` : **string** - orientation of the focus either `up` or `down`\n\nExample:\n\n    python3.7 $HOME/PlanktonScope/scripts/focus.py 650 up\n"
+    },
+    {
+        "id": "52cc1de5.b78e84",
+        "type": "ui_button",
+        "z": "259c8713.b50e28",
+        "name": "stop focus",
+        "group": "fbd92986.1028c8",
+        "order": 5,
+        "width": 4,
+        "height": 1,
+        "passthru": true,
+        "label": "STOP FOCUS",
+        "tooltip": "",
+        "color": "",
+        "bgcolor": "",
+        "icon": "",
+        "payload": "off",
         "payloadType": "str",
-        "repeat": "",
-        "crontab": "",
-        "once": false,
-        "onceDelay": 0.1,
-        "x": 180,
-        "y": 500,
+        "topic": "actuator/wait",
+        "x": 1370,
+        "y": 460,
         "wires": [
             [
-                "61943522.ba3944"
+                "e46763fe.7fc83"
             ]
         ]
     },
     {
-        "id": "53aecd27.a78c64",
+        "id": "39cad6fb.1503ca",
+        "type": "ui_button",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "group": "8cfadfab.327d7",
+        "order": 9,
+        "width": 11,
+        "height": 1,
+        "passthru": false,
+        "label": "Reboot",
+        "tooltip": "",
+        "color": "",
+        "bgcolor": "#AD1625",
+        "icon": "",
+        "payload": "off",
+        "payloadType": "str",
+        "topic": "reboot",
+        "x": 2620,
+        "y": 60,
+        "wires": [
+            [
+                "95b876d4.07c708",
+                "99d61fbe.b244b"
+            ]
+        ]
+    },
+    {
+        "id": "1f53f29b.feee4d",
+        "type": "exec",
+        "z": "259c8713.b50e28",
+        "command": "sudo",
+        "addpay": true,
+        "append": "",
+        "useSpawn": "false",
+        "timer": "2",
+        "oldrc": false,
+        "name": "cmd",
+        "x": 2930,
+        "y": 60,
+        "wires": [
+            [],
+            [],
+            []
+        ]
+    },
+    {
+        "id": "e325458b.95bff8",
+        "type": "ui_button",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "group": "8cfadfab.327d7",
+        "order": 10,
+        "width": 13,
+        "height": 1,
+        "passthru": false,
+        "label": "Shutdown",
+        "tooltip": "",
+        "color": "",
+        "bgcolor": "#AD1625",
+        "icon": "",
+        "payload": "off",
+        "payloadType": "str",
+        "topic": "shutdown",
+        "x": 2620,
+        "y": 120,
+        "wires": [
+            [
+                "95b876d4.07c708",
+                "99d61fbe.b244b"
+            ]
+        ]
+    },
+    {
+        "id": "95b876d4.07c708",
+        "type": "python3-function",
+        "z": "259c8713.b50e28",
+        "name": "action",
+        "func": "#!/usr/bin/python\nimport smbus\nimport time\nbus = smbus.SMBus(1)\ntime.sleep(1)\n#turn off fan RGB\nbus.write_byte_data(0x0d, 0x07, 0x00)\nbus.write_byte_data(0x0d, 0x07, 0x00)\n\nmsg[\"payload\"] = str(msg[\"topic\"])+' now'\nreturn msg",
+        "outputs": 1,
+        "x": 2790,
+        "y": 80,
+        "wires": [
+            [
+                "d348474b.d756a8",
+                "1f53f29b.feee4d"
+            ]
+        ]
+    },
+    {
+        "id": "d348474b.d756a8",
+        "type": "exec",
+        "z": "259c8713.b50e28",
+        "command": "i2cdetect -y 1",
+        "addpay": false,
+        "append": "",
+        "useSpawn": "false",
+        "timer": "1",
+        "oldrc": false,
+        "name": "i2c update",
+        "x": 2950,
+        "y": 120,
+        "wires": [
+            [],
+            [],
+            []
+        ]
+    },
+    {
+        "id": "99d61fbe.b244b",
+        "type": "link out",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "links": [
+            "3517d063.27073"
+        ],
+        "x": 2735,
+        "y": 20,
+        "wires": []
+    },
+    {
+        "id": "cdd1b550.da18e8",
         "type": "mqtt in",
-        "z": "3b5c4e18.844442",
+        "z": "259c8713.b50e28",
         "name": "",
         "topic": "receiver/#",
         "qos": "0",
         "datatype": "auto",
-        "broker": "acc679a.89c4888",
-        "x": 160,
-        "y": 760,
+        "broker": "b77ffa1b.942258",
+        "x": 720,
+        "y": 2000,
         "wires": [
             [
-                "de0c5ae0.cb23b8"
+                "6920281d.dc9b48"
             ]
         ]
     },
     {
-        "id": "de0c5ae0.cb23b8",
+        "id": "6920281d.dc9b48",
         "type": "switch",
-        "z": "3b5c4e18.844442",
+        "z": "259c8713.b50e28",
         "name": "",
         "property": "topic",
         "propertyType": "msg",
@@ -106,26 +2472,42 @@
                 "t": "eq",
                 "v": "receiver/focus",
                 "vt": "str"
+            },
+            {
+                "t": "eq",
+                "v": "receiver/image",
+                "vt": "str"
+            },
+            {
+                "t": "cont",
+                "v": "receiver/segmentation",
+                "vt": "str"
             }
         ],
         "checkall": "true",
         "repair": false,
-        "outputs": 2,
-        "x": 310,
-        "y": 760,
+        "outputs": 4,
+        "x": 870,
+        "y": 2000,
         "wires": [
             [
-                "e34ae39c.6095f8"
+                "2f66e917.fee586"
             ],
             [
-                "7cdd4869.c2a4c8"
+                "363ee47e.ed522c"
+            ],
+            [
+                "45a52868.fe37c8"
+            ],
+            [
+                "469cc189.903d"
             ]
         ]
     },
     {
-        "id": "e34ae39c.6095f8",
+        "id": "2f66e917.fee586",
         "type": "switch",
-        "z": "3b5c4e18.844442",
+        "z": "259c8713.b50e28",
         "name": "",
         "property": "payload",
         "propertyType": "msg",
@@ -149,25 +2531,25 @@
         "checkall": "true",
         "repair": false,
         "outputs": 3,
-        "x": 470,
-        "y": 680,
+        "x": 1050,
+        "y": 1800,
         "wires": [
             [
-                "1dc47b9a.e29d5c"
+                "5c0d0c35.3bcf14"
             ],
             [
-                "f3c1e4cb.18352"
+                "cd01157d.0892d8"
             ],
             [
-                "34ae84d2.cd9864"
+                "68e02bf4.7013e4"
             ]
         ]
     },
     {
-        "id": "1dc47b9a.e29d5c",
+        "id": "5c0d0c35.3bcf14",
         "type": "change",
-        "z": "3b5c4e18.844442",
-        "name": "",
+        "z": "259c8713.b50e28",
+        "name": "The pump has started",
         "rules": [
             {
                 "t": "set",
@@ -182,19 +2564,17 @@
         "from": "",
         "to": "",
         "reg": false,
-        "x": 640,
-        "y": 640,
+        "x": 1440,
+        "y": 1760,
         "wires": [
-            [
-                "6fe70459.267634"
-            ]
+            []
         ]
     },
     {
-        "id": "f3c1e4cb.18352",
+        "id": "cd01157d.0892d8",
         "type": "change",
-        "z": "3b5c4e18.844442",
-        "name": "",
+        "z": "259c8713.b50e28",
+        "name": "The pump has finished.",
         "rules": [
             {
                 "t": "set",
@@ -209,19 +2589,17 @@
         "from": "",
         "to": "",
         "reg": false,
-        "x": 640,
-        "y": 680,
+        "x": 1430,
+        "y": 1800,
         "wires": [
-            [
-                "6fe70459.267634"
-            ]
+            []
         ]
     },
     {
-        "id": "34ae84d2.cd9864",
+        "id": "68e02bf4.7013e4",
         "type": "change",
-        "z": "3b5c4e18.844442",
-        "name": "",
+        "z": "259c8713.b50e28",
+        "name": "The pump has been stopped.",
         "rules": [
             {
                 "t": "set",
@@ -236,33 +2614,16 @@
         "from": "",
         "to": "",
         "reg": false,
-        "x": 640,
-        "y": 720,
+        "x": 1420,
+        "y": 1840,
         "wires": [
-            [
-                "6fe70459.267634"
-            ]
+            []
         ]
     },
     {
-        "id": "6fe70459.267634",
-        "type": "debug",
-        "z": "3b5c4e18.844442",
-        "name": "",
-        "active": true,
-        "tosidebar": true,
-        "console": false,
-        "tostatus": false,
-        "complete": "payload",
-        "targetType": "msg",
-        "x": 850,
-        "y": 680,
-        "wires": []
-    },
-    {
-        "id": "7cdd4869.c2a4c8",
+        "id": "363ee47e.ed522c",
         "type": "switch",
-        "z": "3b5c4e18.844442",
+        "z": "259c8713.b50e28",
         "name": "",
         "property": "payload",
         "propertyType": "msg",
@@ -286,25 +2647,25 @@
         "checkall": "true",
         "repair": false,
         "outputs": 3,
-        "x": 470,
-        "y": 820,
+        "x": 1050,
+        "y": 1920,
         "wires": [
             [
-                "6a11637a.1abcb4"
+                "c088b72f.326d98"
             ],
             [
-                "2a9d2128.1c4d56"
+                "36934381.14442c"
             ],
             [
-                "53ed8d7.37b9bf4"
+                "89329cfe.c8f4"
             ]
         ]
     },
     {
-        "id": "6a11637a.1abcb4",
+        "id": "c088b72f.326d98",
         "type": "change",
-        "z": "3b5c4e18.844442",
-        "name": "",
+        "z": "259c8713.b50e28",
+        "name": "The focus has started",
         "rules": [
             {
                 "t": "set",
@@ -319,19 +2680,17 @@
         "from": "",
         "to": "",
         "reg": false,
-        "x": 640,
-        "y": 780,
+        "x": 1440,
+        "y": 1880,
         "wires": [
-            [
-                "3e0dea7a.524f96"
-            ]
+            []
         ]
     },
     {
-        "id": "2a9d2128.1c4d56",
+        "id": "36934381.14442c",
         "type": "change",
-        "z": "3b5c4e18.844442",
-        "name": "",
+        "z": "259c8713.b50e28",
+        "name": "The focus has finished.",
         "rules": [
             {
                 "t": "set",
@@ -346,19 +2705,17 @@
         "from": "",
         "to": "",
         "reg": false,
-        "x": 640,
-        "y": 820,
+        "x": 1430,
+        "y": 1920,
         "wires": [
-            [
-                "3e0dea7a.524f96"
-            ]
+            []
         ]
     },
     {
-        "id": "53ed8d7.37b9bf4",
+        "id": "89329cfe.c8f4",
         "type": "change",
-        "z": "3b5c4e18.844442",
-        "name": "",
+        "z": "259c8713.b50e28",
+        "name": "The focus has been stopped.",
         "rules": [
             {
                 "t": "set",
@@ -373,31 +2730,1257 @@
         "from": "",
         "to": "",
         "reg": false,
-        "x": 640,
-        "y": 860,
+        "x": 1420,
+        "y": 1960,
+        "wires": [
+            []
+        ]
+    },
+    {
+        "id": "fdb3b132.8b341",
+        "type": "mqtt out",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "topic": "",
+        "qos": "",
+        "retain": "",
+        "broker": "cb2f36fe.1c7038",
+        "x": 1810,
+        "y": 380,
+        "wires": []
+    },
+    {
+        "id": "e46763fe.7fc83",
+        "type": "mqtt out",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "topic": "",
+        "qos": "",
+        "retain": "",
+        "broker": "cb2f36fe.1c7038",
+        "x": 1510,
+        "y": 460,
+        "wires": []
+    },
+    {
+        "id": "61fb057d.3c251c",
+        "type": "mqtt out",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "topic": "",
+        "qos": "",
+        "retain": "",
+        "broker": "cb2f36fe.1c7038",
+        "x": 1810,
+        "y": 580,
+        "wires": []
+    },
+    {
+        "id": "e2a7220a.09d43",
+        "type": "mqtt out",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "topic": "",
+        "qos": "",
+        "retain": "",
+        "broker": "cb2f36fe.1c7038",
+        "x": 1510,
+        "y": 660,
+        "wires": []
+    },
+    {
+        "id": "577f7f72.6b68b",
+        "type": "ui_button",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "group": "8cfadfab.327d7",
+        "order": 7,
+        "width": 0,
+        "height": 0,
+        "passthru": false,
+        "label": "Start Acquisition",
+        "tooltip": "",
+        "color": "",
+        "bgcolor": "",
+        "icon": "",
+        "payload": "",
+        "payloadType": "str",
+        "topic": "actuator/image",
+        "x": 1360,
+        "y": 1560,
         "wires": [
             [
-                "3e0dea7a.524f96"
+                "5218ba30.bbe844"
             ]
         ]
     },
     {
-        "id": "3e0dea7a.524f96",
+        "id": "d5111b87.2a64a8",
+        "type": "ui_text_input",
+        "z": "259c8713.b50e28",
+        "name": "generic_sleep_before",
+        "label": "Sleep duration before acquisition (s)",
+        "tooltip": "",
+        "group": "8cfadfab.327d7",
+        "order": 1,
+        "width": 12,
+        "height": 1,
+        "passthru": true,
+        "mode": "number",
+        "delay": 300,
+        "topic": "generic_sleep_before",
+        "x": 1340,
+        "y": 1360,
+        "wires": [
+            [
+                "3d141631.d548ba"
+            ]
+        ]
+    },
+    {
+        "id": "3d141631.d548ba",
+        "type": "link out",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "links": [
+            "7532db82.1dcf04"
+        ],
+        "x": 1475,
+        "y": 1360,
+        "wires": []
+    },
+    {
+        "id": "74c89905.79d968",
+        "type": "ui_text_input",
+        "z": "259c8713.b50e28",
+        "name": "generic_sleep_during",
+        "label": "Sleep duration between acquisition (s)",
+        "tooltip": "",
+        "group": "8cfadfab.327d7",
+        "order": 2,
+        "width": 12,
+        "height": 1,
+        "passthru": true,
+        "mode": "number",
+        "delay": 300,
+        "topic": "generic_sleep_during",
+        "x": 1340,
+        "y": 1520,
+        "wires": [
+            [
+                "644e95b9.04b7ac"
+            ]
+        ]
+    },
+    {
+        "id": "644e95b9.04b7ac",
+        "type": "link out",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "links": [
+            "7532db82.1dcf04"
+        ],
+        "x": 1475,
+        "y": 1520,
+        "wires": []
+    },
+    {
+        "id": "b50cf2f4.bfb84",
+        "type": "link out",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "links": [
+            "7532db82.1dcf04"
+        ],
+        "x": 1475,
+        "y": 501,
+        "wires": []
+    },
+    {
+        "id": "1e75fd6c.234753",
+        "type": "ui_slider",
+        "z": "259c8713.b50e28",
+        "name": "flowrate",
+        "label": "Flowrate (ml/min)*",
+        "tooltip": "",
+        "group": "707d9797.c8e798",
+        "order": 1,
+        "width": 0,
+        "height": 0,
+        "passthru": true,
+        "outs": "end",
+        "topic": "flowrate",
+        "min": 0,
+        "max": "20",
+        "step": "0.1",
+        "x": 1380,
+        "y": 501,
+        "wires": [
+            [
+                "b50cf2f4.bfb84"
+            ]
+        ]
+    },
+    {
+        "id": "3345fc41.8341b4",
+        "type": "function",
+        "z": "259c8713.b50e28",
+        "name": "get generic.flowrate",
+        "func": "msg.payload = msg.payload.generic_flowrate;\nreturn msg;",
+        "outputs": 1,
+        "noerr": 0,
+        "x": 1030,
+        "y": 501,
+        "wires": [
+            [
+                "1e75fd6c.234753"
+            ]
+        ]
+    },
+    {
+        "id": "32510458.f42f9c",
+        "type": "link in",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "links": [
+            "b368cae7.dd66b8"
+        ],
+        "x": 895,
+        "y": 501,
+        "wires": [
+            [
+                "3345fc41.8341b4"
+            ]
+        ]
+    },
+    {
+        "id": "3cd0a17b.0cc42e",
+        "type": "link out",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "links": [
+            "7532db82.1dcf04"
+        ],
+        "x": 1475,
+        "y": 1440,
+        "wires": []
+    },
+    {
+        "id": "62fea790.2840a8",
+        "type": "ui_text_input",
+        "z": "259c8713.b50e28",
+        "name": "generic_path",
+        "label": "DIrectory to store the images",
+        "tooltip": "",
+        "group": "8cfadfab.327d7",
+        "order": 3,
+        "width": 12,
+        "height": 1,
+        "passthru": true,
+        "mode": "text",
+        "delay": 300,
+        "topic": "generic_path",
+        "x": 1370,
+        "y": 1440,
+        "wires": [
+            [
+                "3cd0a17b.0cc42e"
+            ]
+        ]
+    },
+    {
+        "id": "73e051a8.5875e",
+        "type": "ui_text_input",
+        "z": "259c8713.b50e28",
+        "name": "generic_volume_before",
+        "label": "Volume to pass before to begin",
+        "tooltip": "",
+        "group": "8cfadfab.327d7",
+        "order": 5,
+        "width": 12,
+        "height": 1,
+        "passthru": true,
+        "mode": "number",
+        "delay": 300,
+        "topic": "generic_volume_before",
+        "x": 1330,
+        "y": 1400,
+        "wires": [
+            [
+                "5ea01613.8cc9d8"
+            ]
+        ]
+    },
+    {
+        "id": "5ea01613.8cc9d8",
+        "type": "link out",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "links": [
+            "7532db82.1dcf04"
+        ],
+        "x": 1475,
+        "y": 1400,
+        "wires": []
+    },
+    {
+        "id": "26549e4b.5dbf72",
+        "type": "ui_text_input",
+        "z": "259c8713.b50e28",
+        "name": "generic_nb_frame",
+        "label": "Number of frame per acquisition",
+        "tooltip": "",
+        "group": "8cfadfab.327d7",
+        "order": 4,
+        "width": 12,
+        "height": 1,
+        "passthru": true,
+        "mode": "number",
+        "delay": 300,
+        "topic": "generic_nb_frame",
+        "x": 1350,
+        "y": 1480,
+        "wires": [
+            [
+                "61382d0b.60c534"
+            ]
+        ]
+    },
+    {
+        "id": "61382d0b.60c534",
+        "type": "link out",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "links": [
+            "7532db82.1dcf04"
+        ],
+        "x": 1475,
+        "y": 1480,
+        "wires": []
+    },
+    {
+        "id": "d5df5489.6523e8",
+        "type": "function",
+        "z": "259c8713.b50e28",
+        "name": "get generic_sleep_before",
+        "func": "msg.payload = msg.payload.generic_sleep_before;\nreturn msg;",
+        "outputs": 1,
+        "noerr": 0,
+        "x": 1050,
+        "y": 1360,
+        "wires": [
+            [
+                "d5111b87.2a64a8"
+            ]
+        ]
+    },
+    {
+        "id": "835c262e.00b468",
+        "type": "link in",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "links": [
+            "b368cae7.dd66b8"
+        ],
+        "x": 895,
+        "y": 1360,
+        "wires": [
+            [
+                "d5df5489.6523e8"
+            ]
+        ]
+    },
+    {
+        "id": "9b2fe59b.be48c8",
+        "type": "function",
+        "z": "259c8713.b50e28",
+        "name": "get generic_volume_before",
+        "func": "msg.payload = msg.payload.generic_volume_before;\nreturn msg;",
+        "outputs": 1,
+        "noerr": 0,
+        "x": 1060,
+        "y": 1400,
+        "wires": [
+            [
+                "73e051a8.5875e"
+            ]
+        ]
+    },
+    {
+        "id": "a07dad70.7c48",
+        "type": "link in",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "links": [
+            "b368cae7.dd66b8"
+        ],
+        "x": 895,
+        "y": 1400,
+        "wires": [
+            [
+                "9b2fe59b.be48c8"
+            ]
+        ]
+    },
+    {
+        "id": "54dfe4df.c6837c",
+        "type": "function",
+        "z": "259c8713.b50e28",
+        "name": "get generic_path",
+        "func": "msg.payload = msg.payload.generic_path;\nreturn msg;",
+        "outputs": 1,
+        "noerr": 0,
+        "x": 1030,
+        "y": 1440,
+        "wires": [
+            [
+                "62fea790.2840a8"
+            ]
+        ]
+    },
+    {
+        "id": "9fb166d6.86f908",
+        "type": "link in",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "links": [
+            "b368cae7.dd66b8"
+        ],
+        "x": 895,
+        "y": 1440,
+        "wires": [
+            [
+                "54dfe4df.c6837c"
+            ]
+        ]
+    },
+    {
+        "id": "1aa409a4.7b3866",
+        "type": "function",
+        "z": "259c8713.b50e28",
+        "name": "get generic_nb_frame",
+        "func": "msg.payload = msg.payload.generic_nb_frame;\nreturn msg;",
+        "outputs": 1,
+        "noerr": 0,
+        "x": 1040,
+        "y": 1480,
+        "wires": [
+            [
+                "26549e4b.5dbf72"
+            ]
+        ]
+    },
+    {
+        "id": "51e769aa.92a138",
+        "type": "link in",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "links": [
+            "b368cae7.dd66b8"
+        ],
+        "x": 895,
+        "y": 1480,
+        "wires": [
+            [
+                "1aa409a4.7b3866"
+            ]
+        ]
+    },
+    {
+        "id": "ce6ded0a.8ee37",
+        "type": "function",
+        "z": "259c8713.b50e28",
+        "name": "get generic_sleep_during",
+        "func": "msg.payload = msg.payload.generic_sleep_during;\nreturn msg;",
+        "outputs": 1,
+        "noerr": 0,
+        "x": 1050,
+        "y": 1520,
+        "wires": [
+            [
+                "74c89905.79d968"
+            ]
+        ]
+    },
+    {
+        "id": "3fa20f41.11e61",
+        "type": "link in",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "links": [
+            "b368cae7.dd66b8"
+        ],
+        "x": 895,
+        "y": 1520,
+        "wires": [
+            [
+                "ce6ded0a.8ee37"
+            ]
+        ]
+    },
+    {
+        "id": "5218ba30.bbe844",
+        "type": "function",
+        "z": "259c8713.b50e28",
+        "name": "image.js",
+        "func": "state = global.get(\"state\");\nglobal.set('img_counter',0)\nglobal.set('obj_counter',0)\nif (state == null){state=\"free\"}\n\nvar generic_sleep_before= global.get(\"generic_sleep_before\");\nvar generic_volume_before= global.get(\"generic_volume_before\");\nvar generic_path= global.get(\"generic_path\");\nvar generic_nb_frame= global.get(\"generic_nb_frame\");\nvar generic_sleep_during= global.get(\"generic_sleep_during\");\n\nif (generic_sleep_before === undefined || generic_sleep_before === \"\" || generic_sleep_before === null) {\n    msg.topic = \"Missing entry :\"\n    msg.payload = \"Duration before start the acquisition\";\n    \n}else if (generic_volume_before === undefined || generic_volume_before === \"\" || generic_volume_before === null) {\n    msg.topic = \"Missing entry :\"\n    msg.payload = \"Volume before acquisition\";\n    \n}else if (generic_path === undefined || generic_path === \"\" || generic_path === null) {\n    msg.topic = \"Missing entry :\"\n    msg.payload = \"Directory to store the images\";\n    \n}else if (generic_nb_frame === undefined || generic_nb_frame === \"\" || generic_nb_frame === null) {\n    msg.topic = \"Missing entry :\"\n    msg.payload = \"Number of frames per acquisition\";\n    \n}else if (generic_sleep_during === undefined || generic_sleep_during === \"\" || generic_sleep_during === null) {\n    msg.topic = \"Missing entry :\"\n    msg.payload = \"Duration in between each acquisition\";\n    \n}else {\n    generic_nb_step = 507*generic_volume_before\n    generic_nb_frame=generic_nb_frame-1\n    msg.payload=generic_sleep_before+' '+generic_nb_step+' '+generic_path+' '+generic_nb_frame+' '+generic_sleep_during;\n}\nreturn msg;",
+        "outputs": 1,
+        "noerr": 0,
+        "x": 1520,
+        "y": 1560,
+        "wires": [
+            [
+                "8142bee4.882c2"
+            ]
+        ],
+        "info": "### Focusing\n##### focus.py `nb_step` `orientation`\n\n- `nb_step` : **integer** (from 1 to 100000) - number of step to perform by the stage (about 31um/step)\n- `orientation` : **string** - orientation of the focus either `up` or `down`\n\nExample:\n\n    python3.7 $HOME/PlanktonScope/scripts/focus.py 650 up\n"
+    },
+    {
+        "id": "8142bee4.882c2",
+        "type": "switch",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "property": "topic",
+        "propertyType": "msg",
+        "rules": [
+            {
+                "t": "eq",
+                "v": "actuator/image",
+                "vt": "str"
+            },
+            {
+                "t": "eq",
+                "v": "Missing entry :",
+                "vt": "str"
+            }
+        ],
+        "checkall": "true",
+        "repair": false,
+        "outputs": 2,
+        "x": 1670,
+        "y": 1560,
+        "wires": [
+            [
+                "a4b8aae3.cc3ad8"
+            ],
+            [
+                "54420752.4a4ab8"
+            ]
+        ]
+    },
+    {
+        "id": "54420752.4a4ab8",
+        "type": "ui_toast",
+        "z": "259c8713.b50e28",
+        "position": "dialog",
+        "displayTime": "3",
+        "highlight": "",
+        "sendall": true,
+        "outputs": 1,
+        "ok": "OK",
+        "cancel": "",
+        "raw": false,
+        "topic": "",
+        "name": "",
+        "x": 1830,
+        "y": 1580,
+        "wires": [
+            []
+        ]
+    },
+    {
+        "id": "a4b8aae3.cc3ad8",
+        "type": "mqtt out",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "topic": "",
+        "qos": "",
+        "retain": "",
+        "broker": "cb2f36fe.1c7038",
+        "x": 1810,
+        "y": 1540,
+        "wires": []
+    },
+    {
+        "id": "ca843dd8.d1c62",
+        "type": "exec",
+        "z": "259c8713.b50e28",
+        "command": "python3.7 /home/pi/PlanktonScope/script/mqtt_pump_focus_image.py",
+        "addpay": true,
+        "append": "",
+        "useSpawn": "false",
+        "timer": "",
+        "oldrc": false,
+        "name": "",
+        "x": 440,
+        "y": 280,
+        "wires": [
+            [],
+            [],
+            []
+        ]
+    },
+    {
+        "id": "45a52868.fe37c8",
+        "type": "switch",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "property": "payload",
+        "propertyType": "msg",
+        "rules": [
+            {
+                "t": "eq",
+                "v": "Start",
+                "vt": "str"
+            },
+            {
+                "t": "eq",
+                "v": "Done",
+                "vt": "str"
+            },
+            {
+                "t": "eq",
+                "v": "Interrompted",
+                "vt": "str"
+            },
+            {
+                "t": "cont",
+                "v": "jpg",
+                "vt": "str"
+            }
+        ],
+        "checkall": "true",
+        "repair": false,
+        "outputs": 4,
+        "x": 1050,
+        "y": 2080,
+        "wires": [
+            [
+                "113c9b93.455814"
+            ],
+            [
+                "29a7c6de.6ac2ea"
+            ],
+            [
+                "f1b07b24.b85718"
+            ],
+            [
+                "446a811b.25e4b"
+            ]
+        ]
+    },
+    {
+        "id": "177a91d4.f1016e",
+        "type": "switch",
+        "z": "259c8713.b50e28",
+        "name": "",
+        "property": "payload",
+        "propertyType": "msg",
+        "rules": [
+            {
+                "t": "eq",
+                "v": "Start",
+                "vt": "str"
+            },
+            {
+                "t": "eq",
+                "v": "Done",
+                "vt": "str"
+            }
+        ],
+        "checkall": "true",
+        "repair": false,
+        "outputs": 2,
+        "x": 1190,
+        "y": 2200,
+        "wires": [
+            [
+                "5d717a25.3437e4"
+            ],
+            [
+                "7d713b16.235164"
+            ]
+        ]
+    },
+    {
+        "id": "29a7c6de.6ac2ea",
+        "type": "change",
+        "z": "259c8713.b50e28",
+        "name": "The acquisition has finished.",
+        "rules": [
+            {
+                "t": "set",
+                "p": "payload",
+                "pt": "msg",
+                "to": "The acquisition has finished.",
+                "tot": "str"
+            }
+        ],
+        "action": "",
+        "property": "",
+        "from": "",
+        "to": "",
+        "reg": false,
+        "x": 1420,
+        "y": 2080,
+        "wires": [
+            []
+        ]
+    },
+    {
+        "id": "f1b07b24.b85718",
+        "type": "change",
+        "z": "259c8713.b50e28",
+        "name": "The acquisition has been stopped.",
+        "rules": [
+            {
+                "t": "set",
+                "p": "payload",
+                "pt": "msg",
+                "to": "The acquisition has been stopped.",
+                "tot": "str"
+            }
+        ],
+        "action": "",
+        "property": "",
+        "from": "",
+        "to": "",
+        "reg": false,
+        "x": 1400,
+        "y": 2120,
+        "wires": [
+            []
+        ]
+    },
+    {
+        "id": "113c9b93.455814",
+        "type": "change",
+        "z": "259c8713.b50e28",
+        "name": "The acquisition has started",
+        "rules": [
+            {
+                "t": "set",
+                "p": "payload",
+                "pt": "msg",
+                "to": "The acquisition has started",
+                "tot": "str"
+            }
+        ],
+        "action": "",
+        "property": "",
+        "from": "",
+        "to": "",
+        "reg": false,
+        "x": 1420,
+        "y": 2040,
+        "wires": [
+            []
+        ]
+    },
+    {
+        "id": "446a811b.25e4b",
+        "type": "function",
+        "z": "259c8713.b50e28",
+        "name": "img_counter.js",
+        "func": "img_counter=global.get('img_counter')\nimg_counter=img_counter+1\nglobal.set('img_counter',img_counter)\nmsg.payload = img_counter\nreturn msg;",
+        "outputs": 1,
+        "noerr": 0,
+        "x": 1300,
+        "y": 2160,
+        "wires": [
+            [
+                "57014db.1bbe0b4",
+                "95f4a476.261718"
+            ]
+        ]
+    },
+    {
+        "id": "803b507f.9e38c",
+        "type": "function",
+        "z": "259c8713.b50e28",
+        "name": "obj_counter.js",
+        "func": "obj_counter=global.get('obj_counter')\nobj_counter=obj_counter+1\nglobal.set('obj_counter',obj_counter)\nmsg.payload = obj_counter\nreturn msg;",
+        "outputs": 1,
+        "noerr": 0,
+        "x": 1300,
+        "y": 2280,
+        "wires": [
+            [
+                "6ad4ae5e.62f4a",
+                "95f4a476.261718"
+            ]
+        ]
+    },
+    {
+        "id": "57014db.1bbe0b4",
+        "type": "ui_chart",
+        "z": "259c8713.b50e28",
+        "name": "img_counter",
+        "group": "8cfadfab.327d7",
+        "order": 12,
+        "width": 24,
+        "height": "2",
+        "label": "img_counter",
+        "chartType": "horizontalBar",
+        "legend": "false",
+        "xformat": "HH:mm:ss",
+        "interpolate": "linear",
+        "nodata": "",
+        "dot": false,
+        "ymin": "",
+        "ymax": "",
+        "removeOlder": 1,
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+        "removeOlderUnit": "3600",
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@@ -417,5 +4000,37 @@
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         "willPayload": ""
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