From 653772c3050972ec39db492bf5b9d1cf097dacaf Mon Sep 17 00:00:00 2001 From: Sebastian Wendel Date: Fri, 6 Jan 2023 22:31:04 +0100 Subject: [PATCH] revision of the assembly manual --- docs/hardware/assembly_guide.md | 1890 +++++++++++++++++++++++++++---- 1 file changed, 1680 insertions(+), 210 deletions(-) diff --git a/docs/hardware/assembly_guide.md b/docs/hardware/assembly_guide.md index cb25c50..066d822 100644 --- a/docs/hardware/assembly_guide.md +++ b/docs/hardware/assembly_guide.md @@ -4,339 +4,1809 @@ SPDX-License-Identifier: CC-BY-SA-4.0 # Assembly guide of the PlanktoScope -![Complete](../images/assembly_guide/pictures/complete.webp) +## Content of the Kit -## Step 0: Gather everything you need +![planktoscope-assembly-002.png](../images/assembly_guide/planktoscope-assembly-002.png) -- Laser cut structure -- M12 lenses -- Peristaltic pump and tubing -- Raspberry Pi, motor driver board, GPIO connectors -- Flashed SD card -- Stepper motors -- PiCam and flex cable -- GPIO ribbon connector, headers, HATs, LED -- DC Power terminal -- Magnets -- Super glue -- Standoffs (M2.5), M3 screws and nuts +It is important to ensure that you have all of the necessary components before beginning the assembly of your PlanktoScope. To do so, please check that all bags are present as part of the kit. -Make sure you have your screwdriver kit, soldering iron, and components ready. Also, remember to flash the PlanktoScope image disk on the SD card before installing the Raspberry Pi. +| **Bag** | **Content** | +| ------- | ---------------------------------- | +| **A** | Scews | +| **B** | Tools | +| **C** | Adhesive Pads | +| **D** | Tubing, Glass Cuvettes | +| **E** | Bubbler Pump | +| **F** | Peristaltic Pump | +| **G** | Linear Stepper Motor | +| **H** | Raspberry PI Chip cooler | +| **I** | Raspberry HAT | +| **J** | Camera Lens | +| **K** | MicroSD Card, DC Power Jack Socket | +| **L** | DC Power Supply and Cable | +| **M** | Syringe and Falcon Tube | +| **X1** | Raspberry PI 4 | +| **X2** | Pipet | +| **X3** | Cable ties | +| **X4** | Raspberry PI HQ Camera Modul | +| **X5** | Sandpaper | -If you are not familiar with any process, such as soldering, tapping, or wiring, try and familiarize yourself with the topics first. +If any bags are missing, please go back to the BOM (Bill of Materials) and reorder the required components. -Soldering deals with high heat and potentially toxic materials, so make sure to use the proper precautions. +## About this document -## Step 1: Laser cutting +To read this document, follow these guidelines: -![Step1](../images/assembly_guide/pictures/step1.webp) +### Color codes -Laser cut all components using the .ai file ensuring all cuts are complete. The current design should have a 5mm material thickness. Start by placing laser cut base A on a flat workspace. Make sure all holes are complete, and negative space is clear. +- šŸ”“Ā Look to the color red +- šŸŸ Ā Look to the color orange +- šŸŸ”Ā Look to the color yellow +- šŸŸ¢Ā Look to the color green +- šŸ”µĀ Look to the color blue +- šŸŸ£Ā Look to the color purple + +### Icons + +- šŸ‘Ā Pay attention to this +- āš Ā Be careful with this +- šŸ“œĀ The book says +- šŸŽ¬Ā Action ! +- āŒĀ Don't focus on that location + +As you read through the document, be sure to pay attention to these visual cues to guide you through the build process. + +## Requirements + +### Tools + +![planktoscope-assembly-254.png](../images/assembly_guide/planktoscope-assembly-254.png) + +Content ofĀ **Bag B**: + +- šŸ”“Ā **B1.** Small flat screwdriver 2mm +- šŸŸ Ā **B2.** Razor blade +- šŸŸ”Ā **B3.** Allen key 2mm +- šŸŸ¢ **B4.** Wrenches for standoffs + +### Components + +![planktoscope-assembly-253.png](../images/assembly_guide/planktoscope-assembly-253.png) + +Content ofĀ **Bag A**: + +- šŸŸ¢Ā **A1.** Standoff M2.5 - 6mm - Brass +- šŸ”µĀ **A2.** Standoff M2.5 - 15mm - Ā Brass +- šŸŸ£Ā **A3.** Standoff M2.5 - 16mm - SS +- šŸŸ Ā **A4.** Screw M2.5x5mm CHC - SS +- šŸŸ”Ā **A5.** Screw M2.5x10mm CHC - SS +- šŸ”“Ā **A6.** Screw M3x12mm BHC - SS + +--- + +## Chapter 1: Detach the **Parts** from **panels** by cutting the **tabs** + +![planktoscope-assembly-004.png](../images/assembly_guide/planktoscope-assembly-004.png) +šŸ‘ Locate the **panel S1** and discover the 5 differents **Parts F, P, K, J** and **I**. + +--- + +![planktoscope-assembly-005.png](../images/assembly_guide/planktoscope-assembly-005.png) +šŸŽ¬ Flip your **panel S1**. + +--- + +![planktoscope-assembly-006.png](../images/assembly_guide/planktoscope-assembly-006.png) +šŸ”“ Locate the outer **tabs** on the edges of the different **Parts**. These are typically small projections of material that are used to secure the case parts to the panels. + +--- + +![planktoscope-assembly-007.png](../images/assembly_guide/planktoscope-assembly-007.png) +Gather all the necessary tools. You will need the B2 šŸŸ  **Razor blade** to cut the tabs. + +--- + +![planktoscope-assembly-008.png](../images/assembly_guide/planktoscope-assembly-008.png) + +- šŸŸ£ Use the **razor blade** to cut the outer tabs located on the edges of the different Parts +- āŒ Do not cut the inner tabs present inside the different Parts for now and focus on the outer tabs attaching the Parts to the main panel. + +--- + +![planktoscope-assembly-009.png](../images/assembly_guide/planktoscope-assembly-009.png) + +- Position your **razor blade** on the tab as close to the piece as possible to avoid residual tab after cutting. +- Press firmly on the razor blade, being very careful with your finger, to cut your first tab. +- Make sure you don't damage your table by placing a flat, rigid support under the **S1 panel**. +- Keep going with the other tabs of this piece F. + +Once all of the tabs are cut, gently lift the case parts away from the panels. If the case parts are stuck or difficult to remove, you may need to gently wiggle or pry them loose using a flat tool such as a screwdriver. !!! warning - If you are doing the laser cutting yourself, please take the time to check the calibration of the machine and its power output for the material you are using. A tight fit is needed between the different plates to avoid unwanted play between critical parts. -## Step 2: Standoff installation + Be extremely careful because this is very sharp. -![Step2](../images/assembly_guide/pictures/step2.webp) -![Step2 standoff location](../images/assembly_guide/render/Step2.webp) +--- -Place 8 standoffs (M2.5 6mm) into the designated holes on the laser-cut base A. A pair of pliers make the job more comfortable. Do not overtighten as it is possible to crack the base material. +![planktoscope-assembly-011.png](../images/assembly_guide/planktoscope-assembly-011.png) -![Step3](../images/assembly_guide/pictures/step3.webp) +Once you have removed your **Part** from the main panel by cutting off all the tabs holding it, inspect it for potential residual tabs. -## Step 3: Motor HAT preparation +- šŸŸ£ Here is a **residual tab** that will need to be removed. +- šŸŸ  Here there is no **residual tab** which is perfect. -![Step4](../images/assembly_guide/pictures/step4.webp) +--- -Insert and solder the terminal blocks and headers onto the motor driver PCB. +![planktoscope-assembly-012.png](../images/assembly_guide/planktoscope-assembly-012.png) -![Step5](../images/assembly_guide/pictures/step5.webp) - -Place the motor driver PCB on to the indicated standoffs. - -## Step 4: Magnets setup - -![Step6](../images/assembly_guide/pictures/step6.webp) - -Now is a good time to think about how the magnets will function within the microscope. The magnets in the sample stage will need to attract to the magnets on the flow cell holder. The magnets in the objective holder will need to attract the magnets on the mount. Keep this in mind as you are adding your magnets and tapping your respective M12 holders so your orientation will be correct. - -![Step8](../images/assembly_guide/pictures/step8.webp) - -You can now fix your magnets into their appropriate holes on sample stage **B**. -It is recommended to glue the magnets in place. If the magnets are too large to fit in, the holes can be widened with a handheld drill. However, they should be quite snug in place. Before you glue them in place make sure that the polarity is maintained, as they will be impossible to remove after gluing. - -## Step 5: Sample stage assembly - -![Step9](../images/assembly_guide/pictures/step9.webp) - -Donā€™t be alarmed by the color swap, this is the sample stage **B**. You can now fit the pegs on the driver mounts into the corresponding holes on the sample stage. They should be glued in place with superglue or epoxy. You can spin the shaft to align the driver mounts on the 2 steppers if it helps making the process easier. - -![Step10](../images/assembly_guide/pictures/step10.webp) - -You should now have a sample stage and motor assembly that looks like this. - -## Step 6: Lenses tapping and mounting - -![Step12](../images/assembly_guide/pictures/step12.webp) - -You now need to tap the holes for the M12 lenses in stage and mount **M** and **D**. It is helpful for alignment to do both the objeDtive and tube lens mount together. It is important to do this as straight as possible. A drop of mineral or olive oil can help the process. Be careful to use a right-hand tap (that goes down when turning clockwise). - -![Step13](../images/assembly_guide/pictures/step13.webp) -![Step14](../images/assembly_guide/pictures/step14.webp) - -![Step6-2](../images/assembly_guide/render/step6-2.webp) - -You can now screw the objective lens (the 25mm one) in part **D**. -![Step14](../images/assembly_guide/pictures/step15.webp) - -## Step 7: Camera preparation - -You can now unscrew the lens from the Pi camera, being careful not to disturb the sensor below. -![Image22](../images/assembly_guide/pictures/image22.webp) -![Image30](../images/assembly_guide/pictures/image30.webp) - -## Step 8: Camera mount - -![Step17](../images/assembly_guide/pictures/step17.webp) - -You can mount the camera using the appropriate holes on the camera mount **G**. Be careful to avoid getting oil or dust on the sensor. - -## Step 9: LED preparation - -![Step18](../images/assembly_guide/pictures/step18.webp) - -The LED can then be wired up and put into its mount **F**. If you wire the LED yourself, remember to give enough length to reach the motor driver on the other end of the microscope. You can also add a bit of glue to fix **F** to the motor mount **E** at this time to make assembly easier, though it is not required. +šŸŸ£ Place your razor **blade flat** on the edge of your piece being very careful with your fingers and cut the residual tab. !!! warning - ![Led](../images/assembly_guide/render/led.webp) - This picture shows the correct wiring for the LED. Please make sure the red wire is on the long pin of the LED. + Be extremely careful because this is very sharp. -## Step 10: Vertical slices assembly +--- -You can now start placing the motor mount/LED assembly- **B**, -![Step5](../images/assembly_guide/render/step5.webp) +![planktoscope-assembly-013.png](../images/assembly_guide/planktoscope-assembly-013.png) -**C**, -![Step6](../images/assembly_guide/render/step6.webp) +Repeat the cutting of the **tabs** on all the **Parts F, P, K, J and I** present on the **panel S1**. -**D**, -![Step7](../images/assembly_guide/render/step7.webp) +!!! warning -**E**, -![Step8](../images/assembly_guide/render/step8.webp) + Be extremely careful because this is very sharp. -**F**, -![Step8](../images/assembly_guide/render/step9.webp) +--- -and **G** into the base **A**. +![planktoscope-assembly-014.png](../images/assembly_guide/planktoscope-assembly-014.png) -## Step 11: Pump setup +šŸ”“ Locate the **inner tabs** on the edges of the different **Parts**. -The pump can then be mounted in place on **H**. Thread the wires through the hole with the pump tubing pointed toward the holes on the mount. -![Step19](../images/assembly_guide/pictures/step19.webp) +--- -Fix the pump in place. -![Step20](../images/assembly_guide/pictures/step20.webp) +![planktoscope-assembly-015.png](../images/assembly_guide/planktoscope-assembly-015.png) -## Step 12: Pump mounting +Cut out the **tabs** inside of all the **Parts F, P, K, J and I** detached from the **panel S1**. -You can now mount the pump on base **A**. -![Step15](../images/assembly_guide/render/step15.webp) +Dispose of the cut tabs and any other debris that may have been created during the detachment process. -Your setup should look like this. Don't worry about the wiring, we'll have a look at it in the next step! +!!! warning -![Step21](../images/assembly_guide/pictures/step21.webp) + Be extremely careful because this is very sharp. -## Step 13: Motor HAT wiring +--- -![Step22](../images/assembly_guide/pictures/step22.svg) +![planktoscope-assembly-016.png](../images/assembly_guide/planktoscope-assembly-017.png) -You will now want to wire the steppers and pump to the terminals on the motor driver board. +- āœ… Good way of cutting inner tabs +- āŒ Wrong way of cutting inner tabs -!!! info - The PlanktoScope **uses only bipolar stepper motors** (with 4 wires coming out, and two coils inside), so you need to identify the two wires working together for each coil. The [RepRap Wiki has great information](https://reprap.org/wiki/Stepper_wiring#.22pair.22_wires_on_4_wire_motors) on how to do this, either with a multimeter or without. +--- - You can find more information about stepper motors and how they work in this [document](http://resources.linengineering.com/acton/attachment/3791/f-00ca/1/-/-/-/-/Stepper%20Motor%20Basics.pdf). +![planktoscope-assembly-018.png](../images/assembly_guide/planktoscope-assembly-018.png) -!!! tip - If your wires are too short, you can invert the pump and the focus wiring. However, you will have to remember to change the configuration later on. +Repeat the process on the **panel S2**. -!!! tip - Make sure the wires are properly connected by pulling on them a little. They should not come loose. +!!! warning -## Step 14: Raspberry Pi setup and installation + Be extremely careful because this is very sharp. -![Step24](../images/assembly_guide/pictures/step24.webp) +--- -At this point, you can insert your flashed SD card into your Raspberry Pi. [Consult the guide for flashing your SD card](https://www.planktoscope.org/replicate/assemble-your-kit) before you do this. The heat sink can also be added to the processor. +![planktoscope-assembly-019.png](../images/assembly_guide/planktoscope-assembly-019.png) + +Discover the 11 differents **Parts**. + +--- + +![planktoscope-assembly-020.png](../images/assembly_guide/planktoscope-assembly-020.png) + +Dispose of the cut tabs and any other debris that may have been created during the detachment process. +Inspect the case parts and panels for any damage or imperfections that may have occurred during the detachment process. If any damage is found, it may be necessary to repair or replace the affected parts. + +--- + +## Chapter 2: Place the 4 **Adhesive Pads** under the **Part I** + +![planktoscope-assembly-023.png](../images/assembly_guide/planktoscope-assembly-023.png) +To secure the PlanktoScope on slippery grounds using the adhesive pads, follow these steps. Gather all the necessary materials. You will need: + +- šŸ‘ and Take the Part I +- šŸŸ  Take the four adhesive pads present in the bag A. +- šŸŸ£ Locate the four pockets that will receive the four adhesive pads. + +--- + +![planktoscope-assembly-024.png](../images/assembly_guide/planktoscope-assembly-024.png) + +- Clean the bottom of the case part I. Make sure the surface is free of dirt, debris, and any other substances that may prevent the adhesive pads from sticking properly. +- Remove the paper and place the four adhesive pads in the pockets by pressing firmly on them, sticky-side down. +- Test the stability of the PlanktoScope by gently shaking or tilting it. If it feels secure and does not slip or slide, the adhesive pads have been successfully installed. !!! note - If you choose the Expert path, you still need to flash your sd card, either with the [lite version](https://downloads.raspberrypi.org/raspios_lite_armhf_latest) of Raspberry OS or with the [desktop version](https://downloads.raspberrypi.org/raspios_armhf_latest). -![Step16](../images/assembly_guide/render/step16.webp) + šŸŽ¬ Store this assembly for later. -Mount the Raspberry Pi containing the flashed SD card on the standoffs attached to the laser cut base A. +--- -## Step 15: Standoffs +## Chapter 3: Screw the four **Standoffs** into **Part A** -![Step17](../images/assembly_guide/render/step17.webp) +![planktoscope-assembly-026.png](../images/assembly_guide/planktoscope-assembly-026.png) -Add 8 standoffs (M2.5 15mm) to fix the motor driver board and the Raspberry Pi to the base. +Now it's time to assemble the ground plate for the Raspberry Pi as the PlanktoScope main processing unit. -![Step25](../images/assembly_guide/pictures/step25.webp) +--- -## Step 16: Camera flex cable +![planktoscope-assembly-027.png](../images/assembly_guide/planktoscope-assembly-027.png) -![Step26](../images/assembly_guide/pictures/step26.webp) +- šŸ‘ Grab the **Part A**. +- šŸŸ£ Locate the **four holes** on **Part A**. -At this point you can use the Pi camera flex cable to connect the camera to the Pi. This is done by gently pulling up the tensioners, inserting the cable in the right orientation, then pushing the tensioners back in place to set the cable. Try not to kink or fold the flex cable too much as it is possible to damage it. +--- -## Step 17: Power supply wiring +![planktoscope-assembly-028.png](../images/assembly_guide/planktoscope-assembly-028.png) -![Step29](../images/assembly_guide/pictures/step29.webp) +šŸŸ¢ **A1. Standoff M2.5 - 6mm**- Brass -The power wires can be wired into place on the motor driver board. +--- -!!! tip - Make sure the wires are properly connected by pulling on them a little. They should not come loose. +![planktoscope-assembly-029.png](../images/assembly_guide/planktoscope-assembly-029.png) -## Step 18: Prepare the GPS HAT +šŸŸ¢ **B4**. Wrenches for standoffs -![Step18-1](../images/assembly_guide/render/step18-1.webp) +--- -Insert the battery to power the GPS HAT and solder the terminal mounts in place. +![planktoscope-assembly-030.png](../images/assembly_guide/planktoscope-assembly-030.png) -## Step 19: Install the GPS HAT +- šŸŸ£ Place the Standoff M2.5 - 6mm in the small side of the wrenches for + standoffs B4. +- šŸŸ  Do not use the big side of the wrenches for standoffs since the standoff will be loose in it. -![Step18](../images/assembly_guide/render/step18.webp) +--- -Mount the GPS HAT over the motor driver PCB using the standoffs attached to the laser cut base **A**. +![planktoscope-assembly-031.png](../images/assembly_guide/planktoscope-assembly-031.png) -## Step 20: Install the Fan HAT +- Place the standoff in the hole and start rotating **by hand** in a clockwise direction until secure. +- Then tighten with the wrench. -![Step19](../images/assembly_guide/render/step19.webp) +--- -Place the cooling fan HAT above the Raspberry Pi by mounting it to the standoffs on base **A**. +![planktoscope-assembly-032.png](../images/assembly_guide/planktoscope-assembly-032.png) + +- āœ… Make sure to screw until the standoff is properly inserted in the hole. +- āŒ Do not stop screwing before. + +--- + +![planktoscope-assembly-033.png](../images/assembly_guide/planktoscope-assembly-033.png) + +Keep going for each of the **four holes**. + +--- + +## Chapter 4: Mount the **Heat Sinks** on the **Raspberry Pi** + +![planktoscope-assembly-035.png](../images/assembly_guide/planktoscope-assembly-035.png) + +Locate the **Raspberry Pi 4 Model B** packaging. !!! warning - Be careful to slide the camera flat cable in the slot in the HAT above the connector. -## Step 21: Secure the HATS + Be careful removing it from its packaging. -![Step20](../images/assembly_guide/render/step20.webp) +--- -Secure the cooling fan HAT and GPS HAT by tightening the 8 screws to the standoffs on base A +![planktoscope-assembly-036.png](../images/assembly_guide/planktoscope-assembly-036.png) -## Step 22: Install back panel +Place the **four Heat Sinks** next to your **Raspberry Pi** and mark the locations of the **Heat Sinks** on the **Raspberry Pi**. -![Step21](../images/assembly_guide/render/step21.webp) +- šŸŸ  & šŸ”µ **Small Heat Sinks** +- šŸŸ¢ **Medium Heat Sink** +- šŸŸ£ **Big Heat Sink** -Insert the laser cut border **I** into base **A**. +--- -## Step 23: GPS output connector +![planktoscope-assembly-037.png](../images/assembly_guide/planktoscope-assembly-037.png) -![Step22](../images/assembly_guide/render/step22.webp) +Remove the protective labels under a **Heat Sink** and place the **Heat Sink** on the slot of the **Raspberry Pi**. -Insert the power and GPS connectors into side plate **J**. +--- -## Step 24: Install side panel +![planktoscope-assembly-038.png](../images/assembly_guide/planktoscope-assembly-038.png) -![Step23](../images/assembly_guide/render/step23.webp) +Remove the protective labels under all the **Heat Sinks** and place all the **Heat Sinks** on the slots of the **Raspberry Pi**. -Place the side plate **J** into the designated slots on the base. You can connect the GPS cable to its connector on the board. +--- + +## Chapter 5: Insert the **micro SD card** in the **Raspberry Pi** + +![planktoscope-assembly-040.png](../images/assembly_guide/planktoscope-assembly-040.png) + +- Locate the **SD card adapter** in the **bag K**. +- The **micro SD card** is inserted in the SD card adapter. +- šŸŸ£ Remove the **micro SD card** from the **SD card adapter**. + +--- + +![planktoscope-assembly-041.png](../images/assembly_guide/planktoscope-assembly-041.png) + +- Flip your **Raspberry Pi**. +- šŸŸ  Locate the **micro SD port**. +- šŸŸ£ Insert the **micro SD card** in the **Raspberry Pi**. + +--- + +![planktoscope-assembly-042.png](../images/assembly_guide/planktoscope-assembly-042.png) + +Push the **micro SD card** in the **Raspberry Pi** port to a point of resistance. + +!!! note + + If you notice that the **micro SD card** protrudes about 2mm from its slot, this is normal. + +--- + +## Chapter 6: Mount the **Raspberry Pi** on the **Part A** + +![planktoscope-assembly-044.png](../images/assembly_guide/planktoscope-assembly-044.png) + +- āœ… Make sure to position the Raspberry Pi properly on the four standoffs screwed on the Part A. +- āŒ Do not invert the position of the Raspberry Pi on the four standoffs screwed on the Part A. + +--- + +![planktoscope-assembly-045.png](../images/assembly_guide/planktoscope-assembly-045.png) + +šŸŸ£ **A3. Standoff M2.5 - 16mm** - SS + +--- + +![planktoscope-assembly-046.png](../images/assembly_guide/planktoscope-assembly-046.png) + +Screw by hand a Standoff M2.5 - 16mm on the **Raspberry Pi**. + +--- + +![planktoscope-assembly-047.png](../images/assembly_guide/planktoscope-assembly-047.png) + +- Screw by hand all Standoffs M2.5 - 16mm on the **Raspberry Pi**. +- Make sure you insert all four standoffs by hand and tighten slightly. + +--- + +![planktoscope-assembly-048.png](../images/assembly_guide/planktoscope-assembly-048.png) + +šŸŸ¢ **B4**. Wrenches for standoffs + +--- + +![planktoscope-assembly-049.png](../images/assembly_guide/planktoscope-assembly-049.png) + +- šŸŸ  Secure the Standoff M2.5 - 16 mm - SS **A3** in the big side of the wrenches for standoffs **B4**. +- šŸŸ£ Do not use the small side of the wrenches for standoffs since the standoff wonā€™t fit in it. + +--- + +## Chapter 7: Attach the **Ribbon Cable** to the **Raspberry Pi** + +![planktoscope-assembly-051.png](../images/assembly_guide/planktoscope-assembly-051.png) + +Locate the **Raspberry Pi Camera HQ** packaging. !!! warning - The GPS connector is quite fragile, make sure to align it properly before inserting it. -## Step 25: Install the other side panel + Be careful removing it from its packaging. -![Step25](../images/assembly_guide/render/step25.webp) +--- -Mount the side plate **K** on base **A** using the assigned slots. +![planktoscope-assembly-052.png](../images/assembly_guide/planktoscope-assembly-052.png) -## Step 26: Secure the sides together +Lay your **Raspberry Pi Camera** face down on a suitable surface. -![Step26](../images/assembly_guide/render/step26.webp) - -Secure the laser cut sides with the screws and nuts. - -## Step 27: Secure the sides to the base plate - -![Step27](../images/assembly_guide/render/step27.webp) - -Secure the laser cut sides to the base plate **A** with the screws and nuts. +šŸ”“ The **black connector** is simply a push/pull fit. To disengage the cable, pull the two corners of the black connector down, away from the camera board. It will unclip to about 3mm, make sure you don't pull it off! If you're struggling, try pulling off one corner of the connector at a time. !!! warning - To make this easier, you can turn the assembly upside down or on its side. Be careful when doing so as the plates may fall. -## Step 28: Insert the camera ribbon cable in the camera + Be careful with this, this part is delicate. Lift the black connector gently -![Step28](../images/assembly_guide/pictures/step28.webp) +--- -You can now connect the camera flex cable into the connector on the camera board. Once again, gently pull up the tensioners, insert the cable in the right orientation, and push the tensioners back in place to set the cable. Try not to kink or fold the flex cable too much as it is possible to damage it. +![planktoscope-assembly-053.png](../images/assembly_guide/planktoscope-assembly-053.png) -## Step 29: Assemble the GPIO ribbon cable +Once the connector has been disengaged from the Raspberry Pi camera board, the cable will simply slide out! -If you didn't get an already assembled ribbon cable, you need to build it yourself. +- šŸŸ£ Put aside Camera the Raspberry Pi +- šŸŸ¢ Keep the Ribbon Cable for next step. -The orientation of the connector does not really matter. However, you need to make sure that both connectors are oriented in the same direction and are on the same side of the ribbon. +--- -To assemble, slide the ribbon in its connector and close it off. You need to tighten it really hard. It's very warmly recommended to use a vice to do so. +![planktoscope-assembly-054.png](../images/assembly_guide/planktoscope-assembly-054.png) + +šŸ”“ Locate the **black connector** present on the **Raspberry Pi**. + +--- + +![planktoscope-assembly-055.png](../images/assembly_guide/planktoscope-assembly-055.png) + +šŸ”“ The **black connector** is simply a push/pull fit. To disengage the cable, pull the two corners of the black connector down, away from the camera board. It will unclip to about 3mm, make sure you don't pull it off! If you're struggling, try pulling off one +corner of the connector at a time. !!! warning - Once assembled, the ribbon should NOT look like this: - ![Ribbon wrong](../images/assembly_guide/pictures/ribbon_wrong.webp) - It should rather look like this: - ![Ribbon good](../images/assembly_guide/pictures/ribbon_good.webp) + Be careful, this part is delicate. Gently prise the black connector with nail or fingertip and thumb. -## Step 30: Insert the ribbon cable +--- -![Step28](../images/assembly_guide/render/step28.webp) +![planktoscope-assembly-056.png](../images/assembly_guide/planktoscope-assembly-056.png) -Attach the GPIO ribbon to connect the cooling fan HAT to the GPS HAT. +Insert the **Ribbon Cable** you just detached from the **Raspberry Pi Camera** in the **Raspberry Pi**. -!!! tip - You can try to route the flat ribbon from the camera under the ribbon cable you are connecting now. - ![Step31](../images/assembly_guide/pictures/step31.webp) +- Make sure to insert in as much as you can. +- Blue rectangle on **Ribbon Cable** should face the same direction as the arrow below. -## Step 31: Fluidic assembly +--- -![Step29](../images/assembly_guide/render/step29.webp) +![planktoscope-assembly-057.png](../images/assembly_guide/planktoscope-assembly-057.png) -Feed in the tubing from syringe 1 to form the fluidic path as shown. +šŸ”“ Secure the Ribbon Cable in the Raspberry Pi by pressing firmly on the black connector. -![Step30](../images/assembly_guide/render/step30.webp) +--- -Feed in the tubing from syringe 2 to form the fluidic path as shown +## Chapter 8: Mount the PlanktoScope HAT on the Raspberry Pi -![Step31](../images/assembly_guide/render/step31.webp) +![planktoscope-assembly-059.png](../images/assembly_guide/planktoscope-assembly-059.png) -Feed in a length of tubing as shown through motor mount **H** and illumination mount **FE** +Locate the **PlanktoScope HAT** present in **bag I**. -![Step34](../images/assembly_guide/pictures/step34.webp) +--- -## Step 32: Close your PlanktoScope +![planktoscope-assembly-060.png](../images/assembly_guide/planktoscope-assembly-060.png) + +šŸ”“ Thread the **Ribbon cable** through the **PlanktoScope HAT** slot from the +underside. !!! warning - Take a moment to check your wiring one last time. Also check the routing, make sure the LED wires and the pump stepper wires are in their dedicated channel. -![Step33](../images/assembly_guide/render/step33.webp) + Make sure the two šŸŸ£ black connectors are aligned before threading through the ribbon. -Place the top **L** into the slots on the PlanktoScope body. Secure it in place with screws and nuts. +--- -![Step34](../images/assembly_guide/render/step34.webp) +![planktoscope-assembly-061.png](../images/assembly_guide/planktoscope-assembly-061.png) -## Step 33: Enjoy +šŸ”“ Plug the **PlanktoScope HAT** into the **Raspberry Pi**. -Congratulations on a job well done. You can have some rest, get a tea and some biscuits! +!!! warning -![Step35](../images/assembly_guide/render/step35.webp) + Make sure the two black connectors are aligned before attaching them together. -You can now plug the machine in and test it. If you have choose the Expert's path, now is a good time to [finish setting up your machine](../software/expert_setup.md). +--- -## Step 34: Read the getting started guide +![planktoscope-assembly-062.png](../images/assembly_guide/planktoscope-assembly-062.png) -[A guide to get started with your machine use is available!](../usage/getting_started.md) +Press the **PlanktoScope HAT** against the **Raspberry Pi** until it is no longer possible to move them closer together. + +!!! warning + + Continue to feed through the Ribbon Cable and do not crush it while pressing the PlanktoScope HAT against the standoffs. + +--- + +![planktoscope-assembly-063.png](../images/assembly_guide/planktoscope-assembly-063.png) + +šŸŸ  **A4**. Screw **M2.5X5mm** CHC - SS + +--- + +![planktoscope-assembly-064.png](../images/assembly_guide/planktoscope-assembly-064.png) + +šŸŸ£ Locate the 4 holes on the top of the **PlanktoScope HAT** and insert the four **M2.5X5mm** + +--- + +![planktoscope-assembly-065.png](../images/assembly_guide/planktoscope-assembly-065.png) + +šŸŸ” **B3**. Allen key 2mm + +--- + +![planktoscope-assembly-066.png](../images/assembly_guide/planktoscope-assembly-066.png) + +Screw the four **A4** screws through the **PlanktoScope HAT** onto the **Standoff M2.5 - 16mm**. + +!!! note + + šŸŽ¬ Store this assembly for later. + +## Chapter 9: Place the **Power Socket** on **Part M** + +![planktoscope-assembly-068.png](../images/assembly_guide/planktoscope-assembly-068.png) + +- Locate the **DC Power Jack** from the **Bag K**. +- Remove the **Lock Ring** from the **DC Power Jack** + +--- + +![planktoscope-assembly-069.png](../images/assembly_guide/planktoscope-assembly-069.png) + +--- + +![planktoscope-assembly-070.png](../images/assembly_guide/planktoscope-assembly-070.png) + +- šŸ”“ Lay the **Part M** down and make sure the pockets in these holes are facing upwards. +- šŸŸ£ Locate the **Power Socket** hole on **Part M**. + +--- + +![planktoscope-assembly-071.png](../images/assembly_guide/planktoscope-assembly-071.png) + +šŸ”“ Insert the cable inside of the hole by being sure of the orientation of the **Part M**. + +--- + +![planktoscope-assembly-072.png](../images/assembly_guide/planktoscope-assembly-072.png) + +šŸŸ£ Flip the **Part M** and secure the **DC Power Jack** by hand on the **Part M** by screwing the **Lock Ring**. + +!!! warning + + Make sure the Lock Ring doesnā€™t spin on itself. + +!!! note + + šŸŽ¬ Store this assembly for later. + +## Chapter 10: Mount the **Raspberry Pi Camera HQ** on **Part B** + +![planktoscope-assembly-074.png](../images/assembly_guide/planktoscope-assembly-074.png) + +--- + +![planktoscope-assembly-075.png](../images/assembly_guide/planktoscope-assembly-075.png) + +šŸŸ£ Locate the 4 holes on the top of the **Part B**. + +--- + +![planktoscope-assembly-076.png](../images/assembly_guide/planktoscope-assembly-076.png) + +šŸ”µ **A2**. Standoff M2.5 - 15mm - Brass + +--- + +![planktoscope-assembly-077.png](../images/assembly_guide/planktoscope-assembly-077.png) + +Insert the four **Standoff M2.5 - 15mm**. + +--- + +![planktoscope-assembly-078.png](../images/assembly_guide/planktoscope-assembly-078.png) + +The result should be similar to the picture. + +--- + +![planktoscope-assembly-079.png](../images/assembly_guide/planktoscope-assembly-079.png) + +šŸŸ¢ **B4**. Wrenches for standoffs + +--- + +![planktoscope-assembly-080.png](../images/assembly_guide/planktoscope-assembly-080.png) + +Using the small side of the **Standoff Wrench**, secure the **4 M2.5 - 15mm Standoffs** + +--- + +![planktoscope-assembly-081.png](../images/assembly_guide/planktoscope-assembly-081.png) + +- āœ… Make sure to screw until the **Standoff** is properly tightened into the hole. +- āŒ Do not stop screwing before. + +--- + +![planktoscope-assembly-082.png](../images/assembly_guide/planktoscope-assembly-082.png) + +Locate the **Raspberry Pi Camera HQ** + +--- + +![planktoscope-assembly-083.png](../images/assembly_guide/planktoscope-assembly-083.png) + +Remove the lens cap **Raspberry Pi Camera HQ**. + +![planktoscope-assembly-084.png](../images/assembly_guide/planktoscope-assembly-084.png) + +!!! warning + + Make sure your camera lens is clean. If it is not, gently wipe using cotton swab for this task. + +--- + +![planktoscope-assembly-085.png](../images/assembly_guide/planktoscope-assembly-085.png) + +Place the **Raspberry Pi Camera HQ** on top of the four **Standoffs** installed on **Part B**. + +šŸŸ£Ensure correct orientation of the **Raspberry Pi Camera HQ**. The **black connector** where the **Ribbon Cable** was removed is on the same side as the šŸŸ¢slot circled in green + +--- + +![planktoscope-assembly-086.png](../images/assembly_guide/planktoscope-assembly-086.png) + +šŸŸ  **A4**. Screw M2.5X5mm CHC - SS + +--- + +![planktoscope-assembly-087.png](../images/assembly_guide/planktoscope-assembly-087.png) + +šŸŸ” **B3**. Allen key 2mm + +--- + +![planktoscope-assembly-088.png](../images/assembly_guide/planktoscope-assembly-088.png) + +Use the allen key and tighten the **Raspberry Pi Camera** to the **Standoffs**. + +--- + +![planktoscope-assembly-089.png](../images/assembly_guide/planktoscope-assembly-089.png) + +The result should be similar to the picture. + +!!! note + + šŸŽ¬ Store this assembly for later. + +## Chapter 11: Mount the **Linear Stepper Motor** on **Part E** + +![planktoscope-assembly-091.png](../images/assembly_guide/planktoscope-assembly-091.png) + +Locate the Stepper Motors + +!!! warning + + Avoid touching the metal rods on the Stepper Motors + +!!! info + + You can touch the šŸŸ£ gold stands + +--- + +![planktoscope-assembly-092.png](../images/assembly_guide/planktoscope-assembly-092.png) + +--- + +![planktoscope-assembly-093.png](../images/assembly_guide/planktoscope-assembly-093.png) + +šŸŸ” **A5**. Screw **M2.5X10mm** CHC - SS + +--- + +![planktoscope-assembly-094.png](../images/assembly_guide/planktoscope-assembly-094.png) + +šŸŸ” **B3**. Allen key 2mm + +--- + +![planktoscope-assembly-095.png](../images/assembly_guide/planktoscope-assembly-095.png) + +- šŸ”“ Lay the **Part E** down and make sure the pockets in these holes are + facing upwards. +- šŸŸ£ Locate the four holes on **Part E** and place four M2 Screws in the holes. + +--- + +![planktoscope-assembly-096.png](../images/assembly_guide/planktoscope-assembly-096.png) + +Attach the stepper motors to the screws we have just placed with the šŸ”“ pockets positioned on opposite to the cabling. + +The result should be similar to the picture. + +--- + +![planktoscope-assembly-097.png](../images/assembly_guide/planktoscope-assembly-097.png) + +Use the 2mm allen key to fix the **Stepper Motors**. + +The result should be similar to that picture. + +--- + +![planktoscope-assembly-098.png](../images/assembly_guide/planktoscope-assembly-098.png) + +The result should be similar to the picture. + +--- + +![planktoscope-assembly-099.png](../images/assembly_guide/planktoscope-assembly-099.png) + +Repeat the process on the other side with the other **Stepper Motor**. + +--- + +![planktoscope-assembly-100.png](../images/assembly_guide/planktoscope-assembly-100.png) + +Repeat the process on the other side with the other Stepper Motor. + +--- + +![planktoscope-assembly-101.png](../images/assembly_guide/planktoscope-assembly-101.png) + +The result should be similar to the picture. + +!!! note + + šŸŽ¬ Store this assembly for later. + +--- + +## Chapter 12: Mount theĀ **LED**Ā onĀ **Part G** + +![planktoscope-assembly-103.png](../images/assembly_guide/planktoscope-assembly-103.png) + +- Locate theĀ **LED**Ā andĀ **LED cable**Ā in **Bag K**. +- šŸŸ£Ā The LED will go on the end whereĀ the white plastic connector isĀ smallest. + +--- + +![planktoscope-assembly-104.png](../images/assembly_guide/planktoscope-assembly-104.png) + +Insert theĀ **LED** into the **LED cable**. + +--- + +![planktoscope-assembly-105.png](../images/assembly_guide/planktoscope-assembly-105.png) + +The result should be similar to theĀ picture. + +--- + +![planktoscope-assembly-106.png](../images/assembly_guide/planktoscope-assembly-106.png) + +--- + +![planktoscope-assembly-107.png](../images/assembly_guide/planktoscope-assembly-107.png) + +Locate partĀ **G**. + +--- + +![planktoscope-assembly-108.png](../images/assembly_guide/planktoscope-assembly-108.png) + +šŸŸ£Ā Locate the LED hole onĀ **Part G**. + +--- + +![planktoscope-assembly-109.png](../images/assembly_guide/planktoscope-assembly-109.png) + +WeĀ willĀ nowĀ placeĀ theĀ LEDĀ intoĀ theĀ slotĀ on partĀ **G**. + +--- + +![planktoscope-assembly-110.png](../images/assembly_guide/planktoscope-assembly-110.png) + +!!! warning + + Gently push the LED into the LEDĀ hole located on PartĀ G. It should be a snug fit. + +--- + +![planktoscope-assembly-111.png](../images/assembly_guide/planktoscope-assembly-111.png) + +The result should be similar to theĀ picture. + +!!! info + + šŸŽ¬Ā Store this assembly for later. + +--- + +## Chapter 13: Mount theĀ **Peristaltic Pump**Ā onĀ **Part O** andĀ **Part L** + +![planktoscope-assembly-113.png](../images/assembly_guide/planktoscope-assembly-113.png) + +- Locate theĀ **Kamoer Peristaltic pump**Ā from theĀ **Bag F**. +- šŸŸ£Ā Put aside theĀ **tubing** contained inĀ the little bag. + +--- + +![planktoscope-assembly-114.png](../images/assembly_guide/planktoscope-assembly-114.png) + +--- + +![planktoscope-assembly-115.png](../images/assembly_guide/planktoscope-assembly-115.png) + +šŸŸ¢Ā Insert the cable of theĀ **Peristaltic Pump**Ā into the hole onĀ **Part O**Ā andĀ then insert the motor block assemblyĀ of the pump into it. + +!!! warning + + šŸ‘ Ensure the correct orientation ofĀ Part OĀ and theĀ Peristaltic Pump + +--- + +![planktoscope-assembly-116.png](../images/assembly_guide/planktoscope-assembly-116.png) + +šŸŸ”Ā **A5.** ScrewĀ **M2.5X10mm**Ā CHC - SS + +--- + +![planktoscope-assembly-117.png](../images/assembly_guide/planktoscope-assembly-117.png) + +šŸŸ”Ā **B3.** Allen key 2mm **B3** + +--- + +![planktoscope-assembly-118.png](../images/assembly_guide/planktoscope-assembly-118.png) + +šŸŸ¢Ā Insert the twoĀ **M2.5X10mm**Ā in theĀ two holes. + +--- + +![planktoscope-assembly-119.png](../images/assembly_guide/planktoscope-assembly-119.png) + +Screw the twoĀ **M2.5X10mm**Ā into theĀ two holes. + +--- + +![planktoscope-assembly-120.png](../images/assembly_guide/planktoscope-assembly-120.png) + +--- + +![planktoscope-assembly-121.png](../images/assembly_guide/planktoscope-assembly-121.png) + +šŸ”“Ā LayĀ theĀ **PartĀ L**Ā down and makeĀ sure the pockets in these holes areĀ facing upwards. + +--- + +![planktoscope-assembly-122.png](../images/assembly_guide/planktoscope-assembly-122.png) + +- PlaceĀ theĀ **Peristaltic** **Pump** underneath **part L**, ensuring theĀ correct orientation of these two parts. +- šŸ”“ Insert the **Peristaltic Pump** intoĀ the allocated slot inĀ **Part L**. + +--- + +![planktoscope-assembly-123.png](../images/assembly_guide/planktoscope-assembly-123.png) + +Insert theĀ **Peristaltic Pump**Ā into theĀ allocated slot inĀ **Part L**. + +--- + +![planktoscope-assembly-124.png](../images/assembly_guide/planktoscope-assembly-124.png) + +Insert theĀ **Peristaltic Pump**Ā into theĀ allocated slot inĀ **Part L**. + +--- + +![planktoscope-assembly-125.png](../images/assembly_guide/planktoscope-assembly-125.png) + +- Lay the assembly down. +- šŸ”“Ā Locate theĀ **four different holes.** + +--- + +![planktoscope-assembly-126.png](../images/assembly_guide/planktoscope-assembly-126.png) + +šŸŸ”Ā **A5.**ScrewĀ **M2.5X10mm**Ā CHC - SS + +--- + +![planktoscope-assembly-127.png](../images/assembly_guide/planktoscope-assembly-127.png) + +šŸŸ”Ā **B3.**Allen key 2mm + +--- + +![planktoscope-assembly-128.png](../images/assembly_guide/planktoscope-assembly-128.png) + +Screw the four **M2.5X10mm** in theĀ locatedĀ holesĀ attachingĀ theĀ **PartĀ O**Ā toĀ theĀ **Part L Peristaltic Pump.** + +--- + +![planktoscope-assembly-129.png](../images/assembly_guide/planktoscope-assembly-129.png) +The result should be similar to theĀ picture. + +!!! info + + We will use this part in the next step. + +--- + +## Chapter 14: Spiral wrap the LED and Peristaltic Pump cabling + +![planktoscope-assembly-131.png](../images/assembly_guide/planktoscope-assembly-131.png) + +- Locate the LED and housing, alongĀ with the pump and housing. +- šŸŸ£Ā Locate theĀ **spiral wrap**Ā fromĀ **bag K**. + +--- + +![planktoscope-assembly-132.png](../images/assembly_guide/planktoscope-assembly-132.png) + +- Spiral wrap both sets of cablesĀ together. +- There should be 4 cm (1.5 inches)Ā betweenĀ theĀ connectorsĀ andĀ theĀ startĀ of the spiral wrap. + +--- + +![planktoscope-assembly-133.png](../images/assembly_guide/planktoscope-assembly-133.png) + +Continue wrapping around theĀ cables until you have used all of theĀ spiral wrap, leaving small or no gaps. + +!!! info + + The result should look the same asĀ the picture. + +--- + +![planktoscope-assembly-134.png](../images/assembly_guide/planktoscope-assembly-134.png) + +!!! info + + The result should look the same asĀ the picture. + +!!! note + + šŸŽ¬Ā Store this assembly for later. + +--- + +## Chapter 15: Attaching theĀ **Stepper Motors**Ā to theĀ **Raspberry Pi Camera** + +![planktoscope-assembly-136.png](../images/assembly_guide/planktoscope-assembly-136.png) + +Locate the **Stepper Motors** withĀ mount, and the **Raspberry PiĀ Camera**. + +--- + +![planktoscope-assembly-137.png](../images/assembly_guide/planktoscope-assembly-137.png) +Feed the **Stepper Motor** **cables** intoĀ theĀ slotsĀ eitherĀ sideĀ ofĀ theĀ **RaspberryĀ Pi Camera**. + +!!! warning + + Make sure the orientation isĀ correct and matches the picture. + +--- + +![planktoscope-assembly-138.png](../images/assembly_guide/planktoscope-assembly-138.png) +Feed the **Stepper Motor** **cables** intoĀ theĀ slotsĀ eitherĀ sideĀ ofĀ theĀ **RaspberryĀ Pi Camera**. + +!!! warning + + Make sure the orientation isĀ correct and matches the picture. + +ThenĀ insertĀ theĀ cylindricalĀ partsĀ ofĀ theĀ Stepper Motor into the slots. + +--- + +![planktoscope-assembly-139.png](../images/assembly_guide/planktoscope-assembly-139.png) + +Feed the **Stepper Motor** **cables** intoĀ theĀ slotsĀ eitherĀ sideĀ ofĀ theĀ **RaspberryĀ Pi Camera**. +!!! warning + + Make sure the orientation isĀ correct and matches the picture. + +ThenĀ insertĀ theĀ cylindricalĀ partsĀ ofĀ theĀ Stepper Motor into the slots. + +!!! note + + TheĀ resultĀ shouldĀ beĀ theĀ sameĀ asĀ theĀ picture. + +--- + +## Chapter 16: Connecting theĀ **Raspberry Pi Camera**Ā to theĀ **Raspberry Pi HAT** + +![planktoscope-assembly-141.png](../images/assembly_guide/planktoscope-assembly-141.png) + +- We will need theĀ **Raspberry Pi HAT** with housing and Ribbon Cable alongĀ \ + with theĀ **Raspberry Pi Camera** withĀ Stepper Motors and housing. +- We will be connecting theĀ šŸŸ¢**RibbonĀ Cable**to theĀ šŸŸ”blackĀ **Raspberry Pi Camera**Ā connectorĀ that we removedĀ it from earlier. + +--- + +![planktoscope-assembly-142.png](../images/assembly_guide/planktoscope-assembly-142.png) + +Gently feed theĀ **Ribbon Cable**Ā into theĀ port of theĀ **Camera**. + +!!! warning + + šŸ‘ Ensure the correct orientation ofĀ theĀ Ribbon CableĀ with the blue endĀ facing upwards. + +--- + +![planktoscope-assembly-143.png](../images/assembly_guide/planktoscope-assembly-143.png) +šŸ”“Ā Press down on the blackĀ connector on the Raspberry PiĀ camera board once the RibbonĀ Cable is in position. + +--- + +![planktoscope-assembly-144.png](../images/assembly_guide/planktoscope-assembly-144.png) +šŸ”“Ā **B1.**Small flat screwdriver 2mm + +--- + +![planktoscope-assembly-145.png](../images/assembly_guide/planktoscope-assembly-145.png) + +- Now we will plug in theĀ **StepperĀ Mounts** to theĀ **HAT**. +- šŸ”“The cables for theĀ **Stepper Mounts**Ā will be plugged into theĀ **HAT**Ā šŸŸ£here. + +!!! warning + + Hold tight, a specific order isĀ required. + +--- + +![planktoscope-assembly-146.png](../images/assembly_guide/planktoscope-assembly-146.png) + +Starting with theĀ red cable, insert theĀ cable in the far left port and tightenĀ the screw situated above the port. + +!!! note + + The result should look the same asĀ the picture. + +--- + +![planktoscope-assembly-147.png](../images/assembly_guide/planktoscope-assembly-147.png) + +Repeat this process with the orderĀ pictured here from left to right:Ā šŸ”“ RedĀ šŸŸ” YellowĀ šŸ”µ BlueĀ āš« Black. + +--- + +## Chapter 17: Connect theĀ **LED**Ā andĀ **Peristaltic Pump**Ā to theĀ **Raspberry Pi** + +![planktoscope-assembly-149.png](../images/assembly_guide/planktoscope-assembly-149.png) + +We will now be connecting the **LED**Ā and **Peristaltic Pump** with theĀ **Raspberry Pi**Ā **HAT**Ā andĀ **Camera**. + +--- + +![planktoscope-assembly-150.png](../images/assembly_guide/planktoscope-assembly-150.png) + +Place the LED housing onto theĀ Stepper Mounts. + +!!! warning + + šŸ‘ Ensure correct orientation of bothĀ šŸŸ£ parts by looking at theĀ precut holes. + +--- + +![planktoscope-assembly-151.png](../images/assembly_guide/planktoscope-assembly-151.png) + +!!! info + + The result should be similar to theĀ picture. + +--- + +![planktoscope-assembly-152.png](../images/assembly_guide/planktoscope-assembly-152.png) + +Now we will plug in theĀ **LED** andĀ **Stepper Mount**Ā cables to theĀ **Raspberry Pi**. + +--- + +![planktoscope-assembly-153.png](../images/assembly_guide/planktoscope-assembly-153.png) + +Feed the area of cabling that is notĀ covered by theĀ **spiral wrap**Ā throughĀ the two holes to start. Then threadĀ through to theĀ **spiral wrap**Ā so that itĀ matches the picture. + +--- + +![planktoscope-assembly-154.png](../images/assembly_guide/planktoscope-assembly-154.png) + +We will now plug the cables into theĀ correct ports. + +--- + +![planktoscope-assembly-155.png](../images/assembly_guide/planktoscope-assembly-155.png) + +šŸŸ£Ā The four wires (šŸ”“ RedĀ šŸ”µ Blue šŸŸ¢ Green āš« Black) enter the side port on theĀ Raspberry Pi HAT. + +--- + +![planktoscope-assembly-156.png](../images/assembly_guide/planktoscope-assembly-156.png) + +šŸ”“ The two wires (LED) enter on theĀ port on top of the Raspberry Pi HAT. + +--- + +![planktoscope-assembly-157.png](../images/assembly_guide/planktoscope-assembly-157.png) + +The result should be similar to theĀ picture. + +!!! note + + We will use this assembly in the nextĀ step. + +--- + +## Chapter 18: Connect theĀ **DC Power Jack**Ā to theĀ **Raspberry Pi** + +![planktoscope-assembly-159.png](../images/assembly_guide/planktoscope-assembly-159.png) + +- šŸŸ¢Ā Locate theĀ **DC Power Jack** +- We will plug the DC Power JackĀ into the Raspberry Pi via the šŸ”µ blue port. + +--- + +![planktoscope-assembly-160.png](../images/assembly_guide/planktoscope-assembly-160.png) + +šŸ”“Ā **B1.**Small flat screwdriver 2mm + +--- + +![planktoscope-assembly-161.png](../images/assembly_guide/planktoscope-assembly-161.png) + +- Insert the šŸ”“ red cable of the **DCĀ Power Jack** into the **left side** of theĀ blue port. +- Tighten the screw above. + +--- + +![planktoscope-assembly-162.png](../images/assembly_guide/planktoscope-assembly-162.png) + +- Insert the āš« black cable of the **DCĀ PowerĀ Jack**Ā intoĀ theĀ **rightĀ side**Ā ofĀ theĀ blue port. +- Tighten the screw above. + +--- + +![planktoscope-assembly-163.png](../images/assembly_guide/planktoscope-assembly-163.png) + +!!! info + + The result should be similar to theĀ picture. + +!!! note + + We will use this assembly in the nextĀ step. + +--- + +## Chapter 19: Your PlanktoScope starts to take shape + +![planktoscope-assembly-165.png](../images/assembly_guide/planktoscope-assembly-165.png) + +--- + +![planktoscope-assembly-166.png](../images/assembly_guide/planktoscope-assembly-166.png) + +Locate **Part I**Ā andĀ **Part H**. + +--- + +![planktoscope-assembly-167.png](../images/assembly_guide/planktoscope-assembly-167.png) + +- SlotĀ **Part H**Ā intoĀ **Part I**. +- šŸŸ£Ā Note the orientation.Ā **Part H**Ā goesĀ intoĀ **Part I**at the end with theĀ rectangular slot (as opposed to theĀ rectangle with bulbous hole). +- šŸŸ”Ā Also, the deeper slots onĀ **part H**Ā should be on the upper side. + +--- + +![planktoscope-assembly-168.png](../images/assembly_guide/planktoscope-assembly-168.png) + +šŸ”“ Slot theĀ **Peristaltic Pump**Ā aboveĀ theĀ **LED**. + +--- + +![planktoscope-assembly-169.png](../images/assembly_guide/planktoscope-assembly-169.png) + +!!! note + + The result should be the same as theĀ picture. + +--- + +![planktoscope-assembly-170.png](../images/assembly_guide/planktoscope-assembly-170.png) + +!!! warning + + šŸ‘ Ensure the correct orientation ofĀ the housing and theĀ **Peristaltic Pump**. + +--- + +![planktoscope-assembly-171.png](../images/assembly_guide/planktoscope-assembly-171.png) + +At the other end, slot theĀ **DC PowerĀ Jack**Ā housing adjacent to theĀ **Raspberry Pi**. + +--- + +![planktoscope-assembly-172-00.png](../images/assembly_guide/planktoscope-assembly-172-00.png) +![planktoscope-assembly-172-00.png](../images/assembly_guide/planktoscope-assembly-172-01.png) + +Rotate so that the DC Power Jack isĀ facing upwards. +We will now slot the Raspberry Pi ontoĀ the rest of the housing. + +--- + +![planktoscope-assembly-173.png](../images/assembly_guide/planktoscope-assembly-173.png) + +!!! info + + The result should be similar to thatĀ picture. + +!!! note + + šŸŽ¬Ā Store this assembly for later. + +--- + +## Chapter 20: Inserting screws + +![planktoscope-assembly-175.png](../images/assembly_guide/planktoscope-assembly-175.png) + +šŸŸ£ WeĀ willĀ nowĀ insertĀ eightĀ M3Ā screwsĀ to fasten the housing together. + +!!! note + + šŸŽ¬Ā Store this assembly for later. + +--- + +![planktoscope-assembly-176.png](../images/assembly_guide/planktoscope-assembly-176.png) + +šŸ”“Ā **A6.** Screw M3X12mm BHC - SS + +--- + +![planktoscope-assembly-177.png](../images/assembly_guide/planktoscope-assembly-177.png) + +šŸŸ”Ā **B3.** Allen key 2mm + +--- + +![planktoscope-assembly-178.png](../images/assembly_guide/planktoscope-assembly-178.png) + +!!! info + + The result should be similar to theĀ picture. + +--- + +![planktoscope-assembly-179.png](../images/assembly_guide/planktoscope-assembly-179.png) + +We will now turn over theĀ PlanktoScopeĀ andĀ repeatĀ theĀ processĀ for the underside. + +--- + +![planktoscope-assembly-180.png](../images/assembly_guide/planktoscope-assembly-180.png) + +šŸŸ£Ā Insert eight more M3 screws on theĀ underside. + +--- + +![planktoscope-assembly-181.png](../images/assembly_guide/planktoscope-assembly-181.png) + +!!! info + + The result should be similar to theĀ picture. + +--- + +![planktoscope-assembly-182.png](../images/assembly_guide/planktoscope-assembly-182.png) + +Now turn the PlanktoScope on its side. + +--- + +![planktoscope-assembly-183.png](../images/assembly_guide/planktoscope-assembly-183.png) + +--- + +![planktoscope-assembly-184.png](../images/assembly_guide/planktoscope-assembly-184.png) + +!!! warning + + šŸ‘ Ensure the orientation of yourĀ PlanktoScope andĀ Part KĀ matchesĀ the picture. + +--- + +![planktoscope-assembly-185.png](../images/assembly_guide/planktoscope-assembly-185.png) + +SlotĀ **Part K**Ā onto the rest of theĀ PlanktoScope. + +!!! warning + + šŸŸ¢Ā Ensure the correct orientation. TheĀ result should look the same at theĀ picture. + +--- + +![planktoscope-assembly-186.png](../images/assembly_guide/planktoscope-assembly-186.png) + +Content ofĀ **Bag A**: šŸ”“Ā **A6.** Screw M3X12mm BHC - SS + +--- + +![planktoscope-assembly-187.png](../images/assembly_guide/planktoscope-assembly-187.png) + +Content ofĀ **Bag B**: šŸŸ”Ā **B3.** Allen key 2mm + +--- + +![planktoscope-assembly-188.png](../images/assembly_guide/planktoscope-assembly-188.png) + +šŸŸ£Ā Insert eight more M3 screws on theĀ side to holdĀ **Part K**Ā in place. + +--- + +![planktoscope-assembly-189.png](../images/assembly_guide/planktoscope-assembly-189.png) + +--- + +![planktoscope-assembly-190.png](../images/assembly_guide/planktoscope-assembly-190.png) + +We will now placeĀ **Part J**Ā into positionĀ as the housing for the side. + +!!! warning + + šŸ‘ Ensure your PlanktoScope matchesĀ the orientation in the picture. + +--- + +![planktoscope-assembly-191.png](../images/assembly_guide/planktoscope-assembly-191.png) + +šŸŸ¢ PlaceĀ **Part K**Ā onto the rest of theĀ PlanktoScope and note the positionĀ of the cutout. + +--- + +![planktoscope-assembly-192.png](../images/assembly_guide/planktoscope-assembly-192.png) + +Content ofĀ **Bag A**: šŸ”“Ā **A6.** Screw M3X12mm BHC - SS + +--- + +![planktoscope-assembly-193.png](../images/assembly_guide/planktoscope-assembly-193.png) + +Content ofĀ **Bag B**: šŸŸ”Ā **B3.** Allen key 2mm + +--- + +![planktoscope-assembly-194.png](../images/assembly_guide/planktoscope-assembly-194.png) + +šŸŸ£Ā Insert eight more M3 screws on theĀ underside + +--- + +![planktoscope-assembly-195.png](../images/assembly_guide/planktoscope-assembly-195.png) + +!!! info + + The result should be similar to theĀ picture. + +--- + +![planktoscope-assembly-196.png](../images/assembly_guide/planktoscope-assembly-196.png) + +--- + +![planktoscope-assembly-197.png](../images/assembly_guide/planktoscope-assembly-197.png) + +Content ofĀ **Bag A**: šŸŸ”Ā **A5.** Screw M2.5X10mm CHC - SS + +--- + +![planktoscope-assembly-198.png](../images/assembly_guide/planktoscope-assembly-198.png) + +PlaceĀ theĀ M2.5Ā screwĀ throughĀ **PartĀ N**. ItĀ willĀ actĀ asĀ aĀ **cover**Ā forĀ theĀ electricalĀ inputs. + +--- + +![planktoscope-assembly-199.png](../images/assembly_guide/planktoscope-assembly-199.png) + +- Place theĀ **cover**Ā over the electricalĀ inputs on the PlanktoScope. +- šŸŸ£ The screw will enter the holeĀ located here. + +--- + +![planktoscope-assembly-200.png](../images/assembly_guide/planktoscope-assembly-200.png) + +Using the allen key, tighten the screwĀ so that it is possible to move theĀ cover with light force. + +--- + +![planktoscope-assembly-201.png](../images/assembly_guide/planktoscope-assembly-201.png) + +!!! info + + TheĀ resultĀ shouldĀ beĀ theĀ sameĀ asĀ theĀ picture. + +--- + +## Chapter 21: Insert the tubing in theĀ **Peristaltic Pump** + +![planktoscope-assembly-203.png](../images/assembly_guide/planktoscope-assembly-203.png) + +- OrientateĀ yourĀ PlanktoScopeĀ soĀ thatĀ itĀ matches the picture. +- Twist off the orange top of theĀ PeristalticĀ pumpĀ inĀ anĀ anti-clockwiseĀ direction. + +--- + +![planktoscope-assembly-205.png](../images/assembly_guide/planktoscope-assembly-205.png) + +You can now remove the PeristalticĀ Pump housing andĀ šŸŸ¢ Rotor. + +--- + +![planktoscope-assembly-206.png](../images/assembly_guide/planktoscope-assembly-206.png) + +You can now remove the PeristalticĀ Pump housing andĀ šŸŸ¢ Rotor. + +--- + +![planktoscope-assembly-207.png](../images/assembly_guide/planktoscope-assembly-207.png) + +šŸ”“ Locate theĀ **Tube**Ā for the PeristalticĀ Pump inĀ **Bag F**Ā and remove it from theĀ bag. + +!!! warning + + The tips of the Tubing that areĀ covered by black rubber are veryĀ delicate and easily broken. + +--- + +![planktoscope-assembly-208.png](../images/assembly_guide/planktoscope-assembly-208.png) + +Insert the first plastic arch of theĀ **Tube**Ā into the slot on the Peristaltic PumpĀ \ +housing. + +!!! info + + The result should be similar to theĀ picture. + +--- + +![planktoscope-assembly-209-00.png](../images/assembly_guide/planktoscope-assembly-209-00.png) +![planktoscope-assembly-209-01.png](../images/assembly_guide/planktoscope-assembly-209-01.png) + +Insert theĀ **Rotor**Ā into the housingĀ ensuring the correct orientation. TheĀ hole in the centre should be visible onĀ the underside. + +!!! info + + The result should be similar to theĀ picture. + +--- + +![planktoscope-assembly-210.png](../images/assembly_guide/planktoscope-assembly-210.png) + +Insert theĀ **Rotor**Ā into the housing.Ā Then, thread theĀ **Tube**Ā around theĀ **Rotor**Ā and insert the other plasticĀ arch into the second slot. + +!!! info + + The result should be similar to theĀ picture. + +--- + +![planktoscope-assembly-211.png](../images/assembly_guide/planktoscope-assembly-211.png) + +- Thread theĀ **Tube**Ā around around theĀ **Rotor**Ā and insert the other plasticĀ arch into the second slot. +- This will require stretching theĀ **Tube**Ā slightly. + +--- + +![planktoscope-assembly-212.png](../images/assembly_guide/planktoscope-assembly-212.png) + +!!! info + + The result should be similar to theĀ picture. + +--- + +![planktoscope-assembly-213.png](../images/assembly_guide/planktoscope-assembly-213.png) + +Place the Peristaltic Pump housingĀ back onto the PlanktoScope. + +--- + +![planktoscope-assembly-214.png](../images/assembly_guide/planktoscope-assembly-214.png) + +Achieve the angle shown in theĀ picture between the Peristaltic PumpĀ housing and PlanktoScope mainĀ body. Then, press and twist in aĀ clockwise direction. + +--- + +![planktoscope-assembly-215.png](../images/assembly_guide/planktoscope-assembly-215.png) + +!!! info + + The result should be similar to theĀ picture. + +--- + +![planktoscope-assembly-216.png](../images/assembly_guide/planktoscope-assembly-216.png) + +- šŸŸ£Ā Gently remove the black rubberĀ covers for the Peristaltic PumpĀ connectors by pinching the very tipĀ and pulling away. +- Once complete, locateĀ **Bag D**Ā whichĀ contains tubing. + +--- + +![planktoscope-assembly-217.png](../images/assembly_guide/planktoscope-assembly-217.png) + +Push the small piece of tubing fromĀ **Bag D**Ā over the left-side connector ofĀ theĀ **Peristaltic Pump**. + +--- + +![planktoscope-assembly-218.png](../images/assembly_guide/planktoscope-assembly-218.png) + +Place the long piece of tubing fromĀ **Bag D**Ā over the right-side connectorĀ of theĀ **Peristaltic Pump**. + +--- + +![planktoscope-assembly-219.png](../images/assembly_guide/planktoscope-assembly-219.png) + +Insert the connector fromĀ **Bag D**Ā intoĀ the other end of theĀ **small piece ofĀ tubing**. + +--- + +![planktoscope-assembly-220.png](../images/assembly_guide/planktoscope-assembly-220.png) + +--- + +![planktoscope-assembly-221.png](../images/assembly_guide/planktoscope-assembly-221.png) + +OrientateĀ **Part P**Ā so that the magnetsĀ are face-down on the left-hand side. + +--- + +![planktoscope-assembly-222.png](../images/assembly_guide/planktoscope-assembly-222.png) + +Place **Part P** over the magnetsĀ adjacent to theĀ **Peristaltic Pump**. + +!!! info + + The result should be similar to theĀ picture. + +--- + +![planktoscope-assembly-223.png](../images/assembly_guide/planktoscope-assembly-223.png) + +šŸ”“ FromĀ **Bag M**, Place theĀ **light blue**Ā **Test Tube** in the hole adjacent to theĀ Peristaltic Pump. + +--- + +![planktoscope-assembly-224.png](../images/assembly_guide/planktoscope-assembly-224.png) + +Place theĀ šŸ”µ **dark blue Test Tube**Ā intoĀ the hole situated outside of theĀ PlanktoScope. + +--- + +![planktoscope-assembly-225.png](../images/assembly_guide/planktoscope-assembly-225.png) + +šŸŸ¢Ā Insert the other end of the longĀ piece of tubing into theĀ šŸ”µ **dark blue** **Test Tube**. This will serve as the waste container.Ā The result should look the same asĀ the picture. + +--- + +![planktoscope-assembly-226.png](../images/assembly_guide/planktoscope-assembly-226.png) + +--- + +![planktoscope-assembly-227-00.png](../images/assembly_guide/planktoscope-assembly-227-00.png) +![planktoscope-assembly-227-01.png](../images/assembly_guide/planktoscope-assembly-227-01.png) +![planktoscope-assembly-227-02.png](../images/assembly_guide/planktoscope-assembly-227-02.png) + +- Locate PartĀ **C** +- LocateĀ **25Ā mmĀ cameraĀ lens**Ā andĀ **Lock** **Ring**Ā fromĀ **Bag J**. +- 25MM is printed on the lens. + +--- + +![planktoscope-assembly-228-00.png](../images/assembly_guide/planktoscope-assembly-228-00.png) +![planktoscope-assembly-228-01.png](../images/assembly_guide/planktoscope-assembly-228-01.png) + +- Remove the plastic lens cap. +- SlotĀ theĀ **25Ā mmĀ cameraĀ lens** intoĀ **Part C**. + +!!! info + + The magnets are raised on theĀ side where the lens lays flat. + +!!! warning + + Try not to touch the lens + +Screw theĀ **Lock Ring**Ā onto the lens,Ā **flat-side down**. + +--- + +![planktoscope-assembly-229-00.png](../images/assembly_guide/planktoscope-assembly-229-00.png) +![planktoscope-assembly-229-01.png](../images/assembly_guide/planktoscope-assembly-229-01.png) +![planktoscope-assembly-227-02.png](../images/assembly_guide/planktoscope-assembly-227-02.png) + +- Locate PartĀ **D**. +- LocateĀ **16Ā mmĀ cameraĀ lens**Ā andĀ **LockĀ Ring**Ā fromĀ **Bag J**. +- 16MM is printed on the lens. + +--- + +![planktoscope-assembly-230-00.png](../images/assembly_guide/planktoscope-assembly-230-00.png) +![planktoscope-assembly-230-01.png](../images/assembly_guide/planktoscope-assembly-230-01.png) + +- Remove the plastic lens cap. +- SlotĀ theĀ **16Ā mmĀ cameraĀ lens** into **PartĀ D**. + +!!! info + + TheĀ magnetsĀ areĀ indentedĀ onĀ theĀ side that the lens lays flat. + +!!! warning + + Try not to touch the lens + +Screw theĀ **Lock Ring**Ā onto the lens,Ā **flat-side down**. + +The result should be similar to thatĀ picture. + +--- + +![planktoscope-assembly-231-00.png](../images/assembly_guide/planktoscope-assembly-231-00.png) +![planktoscope-assembly-231-01.png](../images/assembly_guide/planktoscope-assembly-231-01.png) + +PlaceĀ bothĀ **Lenses** (**C**Ā andĀ **D**)Ā togetherĀ so that they flat together and bothĀ lenses are facing each other. + +!!! info + + The result should be similar to theĀ picture. + +--- + +![planktoscope-assembly-232-01.png](../images/assembly_guide/planktoscope-assembly-232-01.png) +![planktoscope-assembly-232-00.png](../images/assembly_guide/planktoscope-assembly-232-00.png) +![planktoscope-assembly-232-02.png](../images/assembly_guide/planktoscope-assembly-232-02.png) + +PlaceĀ theĀ **Lenses**Ā (bothĀ **C**Ā andĀ **D**)Ā intoĀ position, adjacent to the camera. + +The orientation of your PlanktoScopeĀ and **Lenses** should match theĀ pictures. + +--- + +![planktoscope-assembly-233.png](../images/assembly_guide/planktoscope-assembly-233.png) + +- OnceĀ together,Ā placeĀ theĀ lensesĀ (bothĀ **C** and **D**) into position, adjacent toĀ the camera. +- PartĀ **C**Ā /Ā 25mmĀ lensĀ shouldĀ beĀ furthestĀ from the pump (orange piece). + +!!! info + + The result should be similar to theĀ picture. + +--- + +![planktoscope-assembly-234.png](../images/assembly_guide/planktoscope-assembly-234.png) + +--- + +![planktoscope-assembly-235.png](../images/assembly_guide/planktoscope-assembly-235.png) + +!!! info + + The Fluidic Path will have aĀ cardboard protector. + +Please take extra precaution while handlingĀ thisĀ partĀ andĀ avoidĀ touchingĀ the glass element of the piece. + +!!! warning + + The Fluidic Path is very delicate. + +--- + +![planktoscope-assembly-236.png](../images/assembly_guide/planktoscope-assembly-236.png) + +Place the **Tube Clamp** over the longĀ pieceĀ ofĀ tubeĀ atĀ theĀ endĀ ofĀ theĀ **FluidicĀ Path**. + +--- + +![planktoscope-assembly-237.png](../images/assembly_guide/planktoscope-assembly-237.png) + +Place the white clamp over the longĀ pieceĀ ofĀ tubeĀ atĀ theĀ endĀ ofĀ theĀ **Fluidic Path**. + +!!! info + + The result should look the same asĀ the picture. + +--- + +![planktoscope-assembly-238.png](../images/assembly_guide/planktoscope-assembly-238.png) + +Press down on the **Tube Clamp** soĀ that it clicks into place with just oneĀ click. + +!!! info + + The result should look the same asĀ the picture. + +--- + +![planktoscope-assembly-239.png](../images/assembly_guide/planktoscope-assembly-239.png) + +InsertĀ aĀ smallĀ plasticĀ **Connector**Ā intoĀ theĀ endĀ ofĀ theĀ tubing,Ā belowĀ theĀ **TubeĀ Clamp**. + +--- + +![planktoscope-assembly-240.png](../images/assembly_guide/planktoscope-assembly-240.png) + +Make sure the tubing is over theĀ **Connector**. + +!!! info + + The result should look the same asĀ the picture. + +--- + +![planktoscope-assembly-241.png](../images/assembly_guide/planktoscope-assembly-241.png) + +Remove the cardboard protectorĀ from theĀ **Fluidic Path**. + +--- + +![planktoscope-assembly-242.png](../images/assembly_guide/planktoscope-assembly-242.png) + +!!! warning + + A reminder, the glass part of theĀ FluidicĀ PathĀ isĀ veryĀ delicate.Ā PleaseĀ tryĀ not to touch it. + +--- + +![planktoscope-assembly-243.png](../images/assembly_guide/planktoscope-assembly-243.png) + +Place another **Connector** at the endĀ of the tubing. + +--- + +![planktoscope-assembly-244.png](../images/assembly_guide/planktoscope-assembly-244.png) + +TheĀ resultĀ shouldĀ beĀ theĀ sameĀ asĀ theĀ picture. + +--- + +![planktoscope-assembly-245.png](../images/assembly_guide/planktoscope-assembly-245.png) + +āš  Gently place the **Fluidic Path** inĀ the designated indentation onĀ **Part**Ā **F**. + +!!! warning + + Do not touch the glass.Ā  + +- You may have to slightly stretch theĀ tubing to get the Fluidic Path intoĀ position. +- TheĀ resultĀ shouldĀ beĀ theĀ sameĀ asĀ theĀ picture. + +--- + +![planktoscope-assembly-246.png](../images/assembly_guide/planktoscope-assembly-246.png) + +If you have tape available, place aĀ smallĀ pieceĀ atĀ theĀ theĀ bottomĀ ofĀ **Part F** so that the tubing remains fixed inĀ its position. + +Avoid placing tape over the glass. + +!!! info + + The result should be similar to theĀ picture. + +--- + +![planktoscope-assembly-247-01.png](../images/assembly_guide/planktoscope-assembly-247-01.png) +![planktoscope-assembly-247-00.png](../images/assembly_guide/planktoscope-assembly-247-00.png) + +ToĀ insertĀ theĀ syringe,Ā placeĀ fingerĀ andĀ thumb either side of part **F** whereĀ green circle is located. This ensuresĀ the tubing does not rotate while youĀ twist the syringe into position in aĀ clockwise direction. + +--- + +![planktoscope-assembly-248.png](../images/assembly_guide/planktoscope-assembly-248.png) + +!!! info + + The result should be similar to theĀ picture. + +--- + +![planktoscope-assembly-249-00.png](../images/assembly_guide/planktoscope-assembly-249-00.png) +![planktoscope-assembly-249-01.png](../images/assembly_guide/planktoscope-assembly-249-01.png) + +- Place the **Fluidic Path** into positionĀ between the mount and theĀ Peristaltic Pump. +- Connect the magnets to face eachĀ other. + +--- + +![planktoscope-assembly-250.png](../images/assembly_guide/planktoscope-assembly-250.png) + +The result should look the same asĀ the pictures. + +--- + +![planktoscope-assembly-251-00.png](../images/assembly_guide/planktoscope-assembly-251-00.png) +![planktoscope-assembly-251-01.png](../images/assembly_guide/planktoscope-assembly-251-01.png) + +- šŸŸ¢ Connect the **Fluidic Path** to theĀ **Peristaltic Pump** by twisting the twoĀ connectors together. +- TheĀ resultĀ shouldĀ beĀ theĀ sameĀ asĀ theĀ picture. + +--- + +## Build complete! šŸ’Æ šŸ’« + +The next stage is to plug in our PlanktoScopeĀ and connect it to the computer.