# Assembly guide of the PlanktoScope ## Content of the Kit ![planktoscope-assembly-002.png](../images/assembly_guide/planktoscope-assembly-002.png) It is important to ensure that you have all of the necessary components before beginning the assembly of your PlanktoScope. To do so, please check that all bags are present as part of the kit. | **Bag** | **Content** | | ------- | ---------------------------------- | | **A** | Scews | | **B** | Tools | | **C** | Adhesive Pads | | **D** | Tubing, Glass Cuvettes | | **E** | Bubbler Pump | | **F** | Peristaltic Pump | | **G** | Linear Stepper Motor | | **H** | Raspberry PI Chip cooler | | **I** | Raspberry HAT | | **J** | Camera Lens | | **K** | MicroSD Card, DC Power Jack Socket | | **L** | DC Power Supply and Cable | | **M** | Syringe and Falcon Tube | | **X1** | Raspberry PI 4 | | **X2** | Pipet | | **X3** | Cable ties | | **X4** | Raspberry PI HQ Camera Modul | | **X5** | Sandpaper | If any bags are missing, please go back to the BOM (Bill of Materials) and reorder the required components. ## About this document To read this document, follow these guidelines: ### Color codes - šŸ”“Ā Look to the color red - šŸŸ Ā Look to the color orange - šŸŸ”Ā Look to the color yellow - šŸŸ¢Ā Look to the color green - šŸ”µĀ Look to the color blue - šŸŸ£Ā Look to the color purple ### Icons - šŸ‘Ā Pay attention to this - āš Ā Be careful with this - šŸ“œĀ The book says - šŸŽ¬Ā Action ! - āŒĀ Don't focus on that location As you read through the document, be sure to pay attention to these visual cues to guide you through the build process. ## Requirements ### Tools ![planktoscope-assembly-254.png](../images/assembly_guide/planktoscope-assembly-254.png) Content ofĀ **Bag B**: - šŸ”“Ā **B1.** Small flat screwdriver 2mm - šŸŸ Ā **B2.** Razor blade - šŸŸ”Ā **B3.** Allen key 2mm - šŸŸ¢ **B4.** Wrenches for standoffs ### Components ![planktoscope-assembly-253.png](../images/assembly_guide/planktoscope-assembly-253.png) Content ofĀ **Bag A**: - šŸŸ¢Ā **A1.** Standoff M2.5 - 6mm - Brass - šŸ”µĀ **A2.** Standoff M2.5 - 15mm - Ā Brass - šŸŸ£Ā **A3.** Standoff M2.5 - 16mm - SS - šŸŸ Ā **A4.** Screw M2.5x5mm CHC - SS - šŸŸ”Ā **A5.** Screw M2.5x10mm CHC - SS - šŸ”“Ā **A6.** Screw M3x12mm BHC - SS --- ## Chapter 1: Detach the **Parts** from **panels** by cutting the **tabs** ![planktoscope-assembly-004.png](../images/assembly_guide/planktoscope-assembly-004.png) šŸ‘ Locate the **panel S1** and discover the 5 differents **Parts F, P, K, J** and **I**. --- ![planktoscope-assembly-005.png](../images/assembly_guide/planktoscope-assembly-005.png) šŸŽ¬ Flip your **panel S1**. --- ![planktoscope-assembly-006.png](../images/assembly_guide/planktoscope-assembly-006.png) šŸ”“ Locate the outer **tabs** on the edges of the different **Parts**. These are typically small projections of material that are used to secure the case parts to the panels. --- ![planktoscope-assembly-007.png](../images/assembly_guide/planktoscope-assembly-007.png) Gather all the necessary tools. You will need the B2 šŸŸ  **Razor blade** to cut the tabs. --- ![planktoscope-assembly-008.png](../images/assembly_guide/planktoscope-assembly-008.png) - šŸŸ£ Use the **razor blade** to cut the outer tabs located on the edges of the different Parts - āŒ Do not cut the inner tabs present inside the different Parts for now and focus on the outer tabs attaching the Parts to the main panel. --- ![planktoscope-assembly-009.png](../images/assembly_guide/planktoscope-assembly-009.png) - Position your **razor blade** on the tab as close to the piece as possible to avoid residual tab after cutting. - Press firmly on the razor blade, being very careful with your finger, to cut your first tab. - Make sure you don't damage your table by placing a flat, rigid support under the **S1 panel**. - Keep going with the other tabs of this piece F. Once all of the tabs are cut, gently lift the case parts away from the panels. If the case parts are stuck or difficult to remove, you may need to gently wiggle or pry them loose using a flat tool such as a screwdriver. !!! warning Be extremely careful because this is very sharp. --- ![planktoscope-assembly-011.png](../images/assembly_guide/planktoscope-assembly-011.png) Once you have removed your **Part** from the main panel by cutting off all the tabs holding it, inspect it for potential residual tabs. - šŸŸ£ Here is a **residual tab** that will need to be removed. - šŸŸ  Here there is no **residual tab** which is perfect. --- ![planktoscope-assembly-012.png](../images/assembly_guide/planktoscope-assembly-012.png) šŸŸ£ Place your razor **blade flat** on the edge of your piece being very careful with your fingers and cut the residual tab. !!! warning Be extremely careful because this is very sharp. --- ![planktoscope-assembly-013.png](../images/assembly_guide/planktoscope-assembly-013.png) Repeat the cutting of the **tabs** on all the **Parts F, P, K, J and I** present on the **panel S1**. !!! warning Be extremely careful because this is very sharp. --- ![planktoscope-assembly-014.png](../images/assembly_guide/planktoscope-assembly-014.png) šŸ”“ Locate the **inner tabs** on the edges of the different **Parts**. --- ![planktoscope-assembly-015.png](../images/assembly_guide/planktoscope-assembly-015.png) Cut out the **tabs** inside of all the **Parts F, P, K, J and I** detached from the **panel S1**. Dispose of the cut tabs and any other debris that may have been created during the detachment process. !!! warning Be extremely careful because this is very sharp. --- ![planktoscope-assembly-016.png](../images/assembly_guide/planktoscope-assembly-017.png) - āœ… Good way of cutting inner tabs - āŒ Wrong way of cutting inner tabs --- ![planktoscope-assembly-018.png](../images/assembly_guide/planktoscope-assembly-018.png) Repeat the process on the **panel S2**. !!! warning Be extremely careful because this is very sharp. --- ![planktoscope-assembly-019.png](../images/assembly_guide/planktoscope-assembly-019.png) Discover the 11 differents **Parts**. --- ![planktoscope-assembly-020.png](../images/assembly_guide/planktoscope-assembly-020.png) Dispose of the cut tabs and any other debris that may have been created during the detachment process. Inspect the case parts and panels for any damage or imperfections that may have occurred during the detachment process. If any damage is found, it may be necessary to repair or replace the affected parts. --- ## Chapter 2: Place the 4 **Adhesive Pads** under the **Part I** ![planktoscope-assembly-023.png](../images/assembly_guide/planktoscope-assembly-023.png) To secure the PlanktoScope on slippery grounds using the adhesive pads, follow these steps. Gather all the necessary materials. You will need: - Time: 1 min - šŸ‘ and Take the Part I - šŸŸ  Take the four adhesive pads present in the bag A. - šŸŸ£ Locate the four pockets that will receive the four adhesive pads. --- ![planktoscope-assembly-024.png](../images/assembly_guide/planktoscope-assembly-024.png) - Clean the bottom of the case part I. Make sure the surface is free of dirt, debris, and any other substances that may prevent the adhesive pads from sticking properly. - Remove the paper and place the four adhesive pads in the pockets by pressing firmly on them, sticky-side down. - Test the stability of the PlanktoScope by gently shaking or tilting it. If it feels secure and does not slip or slide, the adhesive pads have been successfully installed. !!! note šŸŽ¬ Store this assembly for later. --- ## Chapter 3: Screw the four **Standoffs** into **Part A** ![planktoscope-assembly-026.png](../images/assembly_guide/planktoscope-assembly-026.png) Now it's time to assemble the ground plate for the Raspberry Pi as the PlanktoScope main processing unit. - Time: 5 min --- ![planktoscope-assembly-027.png](../images/assembly_guide/planktoscope-assembly-027.png) - šŸ‘ Grab the **Part A**. - šŸŸ£ Locate the **four holes** on **Part A**. --- ![planktoscope-assembly-028.png](../images/assembly_guide/planktoscope-assembly-028.png) šŸŸ¢ **A1. Standoff M2.5 - 6mm**- Brass --- ![planktoscope-assembly-029.png](../images/assembly_guide/planktoscope-assembly-029.png) šŸŸ¢ **B4**. Wrenches for standoffs --- ![planktoscope-assembly-030.png](../images/assembly_guide/planktoscope-assembly-030.png) - šŸŸ£ Place the Standoff M2.5 - 6mm in the small side of the wrenches for standoffs B4. - šŸŸ  Do not use the big side of the wrenches for standoffs since the standoff will be loose in it. --- ![planktoscope-assembly-031.png](../images/assembly_guide/planktoscope-assembly-031.png) - Place the standoff in the hole and start rotating **by hand** in a clockwise direction until secure. - Then tighten with the wrench. --- ![planktoscope-assembly-032.png](../images/assembly_guide/planktoscope-assembly-032.png) - āœ… Make sure to screw until the standoff is properly inserted in the hole. - āŒ Do not stop screwing before. --- ![planktoscope-assembly-033.png](../images/assembly_guide/planktoscope-assembly-033.png) Keep going for each of the **four holes**. --- ## Chapter 4: Mount the **Heat Sinks** on the **Raspberry Pi** ![planktoscope-assembly-035.png](../images/assembly_guide/planktoscope-assembly-035.png) - Time: 2 min Locate the **Raspberry Pi 4 Model B** packaging. !!! warning Be careful removing it from its packaging. --- ![planktoscope-assembly-036.png](../images/assembly_guide/planktoscope-assembly-036.png) Place the **four Heat Sinks** next to your **Raspberry Pi** and mark the locations of the **Heat Sinks** on the **Raspberry Pi**. - šŸŸ  & šŸ”µ **Small Heat Sinks** - šŸŸ¢ **Medium Heat Sink** - šŸŸ£ **Big Heat Sink** --- ![planktoscope-assembly-037.png](../images/assembly_guide/planktoscope-assembly-037.png) Remove the protective labels under a **Heat Sink** and place the **Heat Sink** on the slot of the **Raspberry Pi**. --- ![planktoscope-assembly-038.png](../images/assembly_guide/planktoscope-assembly-038.png) Remove the protective labels under all the **Heat Sinks** and place all the **Heat Sinks** on the slots of the **Raspberry Pi**. --- ## Chapter 5: Insert the **micro SD card** in the **Raspberry Pi** ![planktoscope-assembly-040.png](../images/assembly_guide/planktoscope-assembly-040.png) - Locate the **SD card adapter** in the **bag K**. - The **micro SD card** is inserted in the SD card adapter. - šŸŸ£ Remove the **micro SD card** from the **SD card adapter**. --- ![planktoscope-assembly-041.png](../images/assembly_guide/planktoscope-assembly-041.png) - Flip your **Raspberry Pi**. - šŸŸ  Locate the **micro SD port**. - šŸŸ£ Insert the **micro SD card** in the **Raspberry Pi**. --- ![planktoscope-assembly-042.png](../images/assembly_guide/planktoscope-assembly-042.png) Push the **micro SD card** in the **Raspberry Pi** port to a point of resistance. !!! note If you notice that the **micro SD card** protrudes about 2mm from its slot, this is normal. --- ## Chapter 6: Mount the **Raspberry Pi** on the **Part A** - Time: 1 min ![planktoscope-assembly-044.png](../images/assembly_guide/planktoscope-assembly-044.png) - āœ… Make sure to position the Raspberry Pi properly on the four standoffs screwed on the Part A. - āŒ Do not invert the position of the Raspberry Pi on the four standoffs screwed on the Part A. --- ![planktoscope-assembly-045.png](../images/assembly_guide/planktoscope-assembly-045.png) šŸŸ£ **A3. Standoff M2.5 - 16mm** - SS --- ![planktoscope-assembly-046.png](../images/assembly_guide/planktoscope-assembly-046.png) Screw by hand a Standoff M2.5 - 16mm on the **Raspberry Pi**. --- ![planktoscope-assembly-047.png](../images/assembly_guide/planktoscope-assembly-047.png) - Screw by hand all Standoffs M2.5 - 16mm on the **Raspberry Pi**. - Make sure you insert all four standoffs by hand and tighten slightly. --- ![planktoscope-assembly-048.png](../images/assembly_guide/planktoscope-assembly-048.png) šŸŸ¢ **B4**. Wrenches for standoffs --- ![planktoscope-assembly-049.png](../images/assembly_guide/planktoscope-assembly-049.png) - šŸŸ  Secure the Standoff M2.5 - 16 mm - SS **A3** in the big side of the wrenches for standoffs **B4**. - šŸŸ£ Do not use the small side of the wrenches for standoffs since the standoff wonā€™t fit in it. --- ## Chapter 7: Attach the **Ribbon Cable** to the **Raspberry Pi** - Time: 2 min ![planktoscope-assembly-051.png](../images/assembly_guide/planktoscope-assembly-051.png) Locate the **Raspberry Pi Camera HQ** packaging. !!! warning Be careful removing it from its packaging. --- ![planktoscope-assembly-052.png](../images/assembly_guide/planktoscope-assembly-052.png) Lay your **Raspberry Pi Camera** face down on a suitable surface. šŸ”“ The **black connector** is simply a push/pull fit. To disengage the cable, pull the two corners of the black connector down, away from the camera board. It will unclip to about 3mm, make sure you don't pull it off! If you're struggling, try pulling off one corner of the connector at a time. !!! warning Be careful with this, this part is delicate. Lift the black connector gently --- ![planktoscope-assembly-053.png](../images/assembly_guide/planktoscope-assembly-053.png) Once the connector has been disengaged from the Raspberry Pi camera board, the cable will simply slide out! - šŸŸ£ Put aside Camera the Raspberry Pi - šŸŸ¢ Keep the Ribbon Cable for next step. --- ![planktoscope-assembly-054.png](../images/assembly_guide/planktoscope-assembly-054.png) šŸ”“ Locate the **black connector** present on the **Raspberry Pi**. --- ![planktoscope-assembly-055.png](../images/assembly_guide/planktoscope-assembly-055.png) šŸ”“ The **black connector** is simply a push/pull fit. To disengage the cable, pull the two corners of the black connector down, away from the camera board. It will unclip to about 3mm, make sure you don't pull it off! If you're struggling, try pulling off one corner of the connector at a time. !!! warning Be careful, this part is delicate. Gently prise the black connector with nail or fingertip and thumb. --- ![planktoscope-assembly-056.png](../images/assembly_guide/planktoscope-assembly-056.png) Insert the **Ribbon Cable** you just detached from the **Raspberry Pi Camera** in the **Raspberry Pi**. - Make sure to insert in as much as you can. - Blue rectangle on **Ribbon Cable** should face the same direction as the arrow below. --- ![planktoscope-assembly-057.png](../images/assembly_guide/planktoscope-assembly-057.png) šŸ”“ Secure the Ribbon Cable in the Raspberry Pi by pressing firmly on the black connector. --- ## Chapter 8: Mount the PlanktoScope HAT on the Raspberry Pi ![planktoscope-assembly-059.png](../images/assembly_guide/planktoscope-assembly-059.png) - Time: 2 min Locate the **PlanktoScope HAT** present in **bag I**. --- ![planktoscope-assembly-060.png](../images/assembly_guide/planktoscope-assembly-060.png) šŸ”“ Thread the **Ribbon cable** through the **PlanktoScope HAT** slot from the underside. !!! warning Make sure the two šŸŸ£ black connectors are aligned before threading through the ribbon. --- ![planktoscope-assembly-061.png](../images/assembly_guide/planktoscope-assembly-061.png) šŸ”“ Plug the **PlanktoScope HAT** into the **Raspberry Pi**. !!! warning Make sure the two black connectors are aligned before attaching them together. --- ![planktoscope-assembly-062.png](../images/assembly_guide/planktoscope-assembly-062.png) Press the **PlanktoScope HAT** against the **Raspberry Pi** until it is no longer possible to move them closer together. !!! warning Continue to feed through the Ribbon Cable and do not crush it while pressing the PlanktoScope HAT against the standoffs. --- ![planktoscope-assembly-063.png](../images/assembly_guide/planktoscope-assembly-063.png) šŸŸ  **A4**. Screw **M2.5X5mm** CHC - SS --- ![planktoscope-assembly-064.png](../images/assembly_guide/planktoscope-assembly-064.png) šŸŸ£ Locate the 4 holes on the top of the **PlanktoScope HAT** and insert the four **M2.5X5mm** --- ![planktoscope-assembly-065.png](../images/assembly_guide/planktoscope-assembly-065.png) šŸŸ” **B3**. Allen key 2mm --- ![planktoscope-assembly-066.png](../images/assembly_guide/planktoscope-assembly-066.png) Screw the four **A4** screws through the **PlanktoScope HAT** onto the **Standoff M2.5 - 16mm**. !!! note šŸŽ¬ Store this assembly for later. ## Chapter 9: Place the **Power Socket** on **Part M** ![planktoscope-assembly-068.png](../images/assembly_guide/planktoscope-assembly-068.png) - Time: 2 min - Locate the **DC Power Jack** from the **Bag K**. - Remove the **Lock Ring** from the **DC Power Jack** --- ![planktoscope-assembly-069.png](../images/assembly_guide/planktoscope-assembly-069.png) --- ![planktoscope-assembly-070.png](../images/assembly_guide/planktoscope-assembly-070.png) - šŸ”“ Lay the **Part M** down and make sure the pockets in these holes are facing upwards. - šŸŸ£ Locate the **Power Socket** hole on **Part M**. --- ![planktoscope-assembly-071.png](../images/assembly_guide/planktoscope-assembly-071.png) šŸ”“ Insert the cable inside of the hole by being sure of the orientation of the **Part M**. --- ![planktoscope-assembly-072.png](../images/assembly_guide/planktoscope-assembly-072.png) šŸŸ£ Flip the **Part M** and secure the **DC Power Jack** by hand on the **Part M** by screwing the **Lock Ring**. !!! warning Make sure the Lock Ring doesnā€™t spin on itself. !!! note šŸŽ¬ Store this assembly for later. ## Chapter 10: Mount the **Raspberry Pi Camera HQ** on **Part B** ![planktoscope-assembly-074.png](../images/assembly_guide/planktoscope-assembly-074.png) - Time: 2 min --- ![planktoscope-assembly-075.png](../images/assembly_guide/planktoscope-assembly-075.png) šŸŸ£ Locate the 4 holes on the top of the **Part B**. --- ![planktoscope-assembly-076.png](../images/assembly_guide/planktoscope-assembly-076.png) šŸ”µ **A2**. Standoff M2.5 - 15mm - Brass --- ![planktoscope-assembly-077.png](../images/assembly_guide/planktoscope-assembly-077.png) Insert the four **Standoff M2.5 - 15mm**. --- ![planktoscope-assembly-078.png](../images/assembly_guide/planktoscope-assembly-078.png) The result should be similar to the picture. --- ![planktoscope-assembly-079.png](../images/assembly_guide/planktoscope-assembly-079.png) šŸŸ¢ **B4**. Wrenches for standoffs --- ![planktoscope-assembly-080.png](../images/assembly_guide/planktoscope-assembly-080.png) Using the small side of the **Standoff Wrench**, secure the **4 M2.5 - 15mm Standoffs** --- ![planktoscope-assembly-081.png](../images/assembly_guide/planktoscope-assembly-081.png) - āœ… Make sure to screw until the **Standoff** is properly tightened into the hole. - āŒ Do not stop screwing before. --- ![planktoscope-assembly-082.png](../images/assembly_guide/planktoscope-assembly-082.png) Locate the **Raspberry Pi Camera HQ** --- ![planktoscope-assembly-083.png](../images/assembly_guide/planktoscope-assembly-083.png) Remove the lens cap **Raspberry Pi Camera HQ**. ![planktoscope-assembly-084.png](../images/assembly_guide/planktoscope-assembly-084.png) !!! warning Make sure your camera lens is clean. If it is not, gently wipe using cotton swab for this task. --- ![planktoscope-assembly-085.png](../images/assembly_guide/planktoscope-assembly-085.png) Place the **Raspberry Pi Camera HQ** on top of the four **Standoffs** installed on **Part B**. šŸŸ£Ensure correct orientation of the **Raspberry Pi Camera HQ**. The **black connector** where the **Ribbon Cable** was removed is on the same side as the šŸŸ¢slot circled in green --- ![planktoscope-assembly-086.png](../images/assembly_guide/planktoscope-assembly-086.png) šŸŸ  **A4**. Screw M2.5X5mm CHC - SS --- ![planktoscope-assembly-087.png](../images/assembly_guide/planktoscope-assembly-087.png) šŸŸ” **B3**. Allen key 2mm --- ![planktoscope-assembly-088.png](../images/assembly_guide/planktoscope-assembly-088.png) Use the allen key and tighten the **Raspberry Pi Camera** to the **Standoffs**. --- ![planktoscope-assembly-089.png](../images/assembly_guide/planktoscope-assembly-089.png) The result should be similar to the picture. !!! note šŸŽ¬ Store this assembly for later. ## Chapter 11: Mount the **Linear Stepper Motor** on **Part E** ![planktoscope-assembly-091.png](../images/assembly_guide/planktoscope-assembly-091.png) Locate the Stepper Motors !!! warning Avoid touching the metal rods on the Stepper Motors !!! info You can touch the šŸŸ£ gold stands --- ![planktoscope-assembly-092.png](../images/assembly_guide/planktoscope-assembly-092.png) --- ![planktoscope-assembly-093.png](../images/assembly_guide/planktoscope-assembly-093.png) šŸŸ” **A5**. Screw **M2.5X10mm** CHC - SS --- ![planktoscope-assembly-094.png](../images/assembly_guide/planktoscope-assembly-094.png) šŸŸ” **B3**. Allen key 2mm --- ![planktoscope-assembly-095.png](../images/assembly_guide/planktoscope-assembly-095.png) - šŸ”“ Lay the **Part E** down and make sure the pockets in these holes are facing upwards. - šŸŸ£ Locate the four holes on **Part E** and place four M2 Screws in the holes. --- ![planktoscope-assembly-096.png](../images/assembly_guide/planktoscope-assembly-096.png) Attach the stepper motors to the screws we have just placed with the šŸ”“ pockets positioned on opposite to the cabling. The result should be similar to the picture. --- ![planktoscope-assembly-097.png](../images/assembly_guide/planktoscope-assembly-097.png) Use the 2mm allen key to fix the **Stepper Motors**. The result should be similar to that picture. --- ![planktoscope-assembly-098.png](../images/assembly_guide/planktoscope-assembly-098.png) The result should be similar to the picture. --- ![planktoscope-assembly-099.png](../images/assembly_guide/planktoscope-assembly-099.png) Repeat the process on the other side with the other **Stepper Motor**. --- ![planktoscope-assembly-100.png](../images/assembly_guide/planktoscope-assembly-100.png) Repeat the process on the other side with the other Stepper Motor. --- ![planktoscope-assembly-101.png](../images/assembly_guide/planktoscope-assembly-101.png) The result should be similar to the picture. !!! note šŸŽ¬ Store this assembly for later. --- ## Chapter 12: Mount theĀ **LED**Ā onĀ **Part G** ![planktoscope-assembly-103.png](../images/assembly_guide/planktoscope-assembly-103.png) - Locate theĀ **LED**Ā andĀ **LED cable**Ā in **Bag K**. - šŸŸ£Ā The LED will go on the end whereĀ the white plastic connector isĀ smallest. --- ![planktoscope-assembly-104.png](../images/assembly_guide/planktoscope-assembly-104.png) Insert theĀ **LED** into the **LED cable**. --- ![planktoscope-assembly-105.png](../images/assembly_guide/planktoscope-assembly-105.png) The result should be similar to theĀ picture. --- ![planktoscope-assembly-106.png](../images/assembly_guide/planktoscope-assembly-106.png) --- ![planktoscope-assembly-107.png](../images/assembly_guide/planktoscope-assembly-107.png) Locate partĀ **G**. --- ![planktoscope-assembly-108.png](../images/assembly_guide/planktoscope-assembly-108.png) šŸŸ£Ā Locate the LED hole onĀ **Part G**. --- ![planktoscope-assembly-109.png](../images/assembly_guide/planktoscope-assembly-109.png) WeĀ willĀ nowĀ placeĀ theĀ LEDĀ intoĀ theĀ slotĀ on partĀ **G**. --- ![planktoscope-assembly-110.png](../images/assembly_guide/planktoscope-assembly-110.png) !!! warning Gently push the LED into the LEDĀ hole located on PartĀ G. It should be a snug fit. --- ![planktoscope-assembly-111.png](../images/assembly_guide/planktoscope-assembly-111.png) The result should be similar to theĀ picture. !!! info šŸŽ¬Ā Store this assembly for later. --- ## Chapter 13: Mount theĀ **Peristaltic Pump**Ā onĀ **Part O** andĀ **Part L** ![planktoscope-assembly-113.png](../images/assembly_guide/planktoscope-assembly-113.png) - Locate theĀ **Kamoer Peristaltic pump**Ā from theĀ **Bag F**. - šŸŸ£Ā Put aside theĀ **tubing** contained inĀ the little bag. --- ![planktoscope-assembly-114.png](../images/assembly_guide/planktoscope-assembly-114.png) --- ![planktoscope-assembly-115.png](../images/assembly_guide/planktoscope-assembly-115.png) šŸŸ¢Ā Insert the cable of theĀ **Peristaltic Pump**Ā into the hole onĀ **Part O**Ā andĀ then insert the motor block assemblyĀ of the pump into it. !!! warning šŸ‘ Ensure the correct orientation ofĀ Part OĀ and theĀ Peristaltic Pump --- ![planktoscope-assembly-116.png](../images/assembly_guide/planktoscope-assembly-116.png) šŸŸ”Ā **A5.** ScrewĀ **M2.5X10mm**Ā CHC - SS --- ![planktoscope-assembly-117.png](../images/assembly_guide/planktoscope-assembly-117.png) šŸŸ”Ā **B3.** Allen key 2mm **B3** --- ![planktoscope-assembly-118.png](../images/assembly_guide/planktoscope-assembly-118.png) šŸŸ¢Ā Insert the twoĀ **M2.5X10mm**Ā in theĀ two holes. --- ![planktoscope-assembly-119.png](../images/assembly_guide/planktoscope-assembly-119.png) Screw the twoĀ **M2.5X10mm**Ā into theĀ two holes. --- ![planktoscope-assembly-120.png](../images/assembly_guide/planktoscope-assembly-120.png) --- ![planktoscope-assembly-121.png](../images/assembly_guide/planktoscope-assembly-121.png) šŸ”“Ā LayĀ theĀ **PartĀ L**Ā down and makeĀ sure the pockets in these holes areĀ facing upwards. --- ![planktoscope-assembly-122.png](../images/assembly_guide/planktoscope-assembly-122.png) - PlaceĀ theĀ **Peristaltic** **Pump** underneath **part L**, ensuring theĀ correct orientation of these two parts. - šŸ”“ Insert the **Peristaltic Pump** intoĀ the allocated slot inĀ **Part L**. --- ![planktoscope-assembly-123.png](../images/assembly_guide/planktoscope-assembly-123.png) Insert theĀ **Peristaltic Pump**Ā into theĀ allocated slot inĀ **Part L**. --- ![planktoscope-assembly-124.png](../images/assembly_guide/planktoscope-assembly-124.png) Insert theĀ **Peristaltic Pump**Ā into theĀ allocated slot inĀ **Part L**. --- ![planktoscope-assembly-125.png](../images/assembly_guide/planktoscope-assembly-125.png) - Lay the assembly down. - šŸ”“Ā Locate theĀ **four different holes.** --- ![planktoscope-assembly-126.png](../images/assembly_guide/planktoscope-assembly-126.png) šŸŸ”Ā **A5.**ScrewĀ **M2.5X10mm**Ā CHC - SS --- ![planktoscope-assembly-127.png](../images/assembly_guide/planktoscope-assembly-127.png) šŸŸ”Ā **B3.**Allen key 2mm --- ![planktoscope-assembly-128.png](../images/assembly_guide/planktoscope-assembly-128.png) Screw the four **M2.5X10mm** in theĀ locatedĀ holesĀ attachingĀ theĀ **PartĀ O**Ā toĀ theĀ **Part L Peristaltic Pump.** --- ![planktoscope-assembly-129.png](../images/assembly_guide/planktoscope-assembly-129.png) The result should be similar to theĀ picture. !!! info We will use this part in the next step. --- ## Chapter 14: Spiral wrap the LED and Peristaltic Pump cabling ![planktoscope-assembly-131.png](../images/assembly_guide/planktoscope-assembly-131.png) - Locate the LED and housing, alongĀ with the pump and housing. - šŸŸ£Ā Locate theĀ **spiral wrap**Ā fromĀ **bag K**. --- ![planktoscope-assembly-132.png](../images/assembly_guide/planktoscope-assembly-132.png) - Spiral wrap both sets of cablesĀ together. - There should be 4 cm (1.5 inches)Ā betweenĀ theĀ connectorsĀ andĀ theĀ startĀ of the spiral wrap. --- ![planktoscope-assembly-133.png](../images/assembly_guide/planktoscope-assembly-133.png) Continue wrapping around theĀ cables until you have used all of theĀ spiral wrap, leaving small or no gaps. !!! info The result should look the same asĀ the picture. --- ![planktoscope-assembly-134.png](../images/assembly_guide/planktoscope-assembly-134.png) !!! info The result should look the same asĀ the picture. !!! note šŸŽ¬Ā Store this assembly for later. --- ## Chapter 15: Attaching theĀ **Stepper Motors**Ā to theĀ **Raspberry Pi Camera** ![planktoscope-assembly-136.png](../images/assembly_guide/planktoscope-assembly-136.png) Locate the **Stepper Motors** withĀ mount, and the **Raspberry PiĀ Camera**. --- ![planktoscope-assembly-137.png](../images/assembly_guide/planktoscope-assembly-137.png) Feed the **Stepper Motor** **cables** intoĀ theĀ slotsĀ eitherĀ sideĀ ofĀ theĀ **RaspberryĀ Pi Camera**. !!! warning Make sure the orientation isĀ correct and matches the picture. --- ![planktoscope-assembly-138.png](../images/assembly_guide/planktoscope-assembly-138.png) Feed the **Stepper Motor** **cables** intoĀ theĀ slotsĀ eitherĀ sideĀ ofĀ theĀ **RaspberryĀ Pi Camera**. !!! warning Make sure the orientation isĀ correct and matches the picture. ThenĀ insertĀ theĀ cylindricalĀ partsĀ ofĀ theĀ Stepper Motor into the slots. --- ![planktoscope-assembly-139.png](../images/assembly_guide/planktoscope-assembly-139.png) Feed the **Stepper Motor** **cables** intoĀ theĀ slotsĀ eitherĀ sideĀ ofĀ theĀ **RaspberryĀ Pi Camera**. !!! warning Make sure the orientation isĀ correct and matches the picture. ThenĀ insertĀ theĀ cylindricalĀ partsĀ ofĀ theĀ Stepper Motor into the slots. !!! note TheĀ resultĀ shouldĀ beĀ theĀ sameĀ asĀ theĀ picture. --- ## Chapter 16: Connecting theĀ **Raspberry Pi Camera**Ā to theĀ **Raspberry Pi HAT** ![planktoscope-assembly-141.png](../images/assembly_guide/planktoscope-assembly-141.png) - We will need theĀ **Raspberry Pi HAT** with housing and Ribbon Cable alongĀ \ with theĀ **Raspberry Pi Camera** withĀ Stepper Motors and housing. - We will be connecting theĀ šŸŸ¢**RibbonĀ Cable**to theĀ šŸŸ”blackĀ **Raspberry Pi Camera**Ā connectorĀ that we removedĀ it from earlier. --- ![planktoscope-assembly-142.png](../images/assembly_guide/planktoscope-assembly-142.png) Gently feed theĀ **Ribbon Cable**Ā into theĀ port of theĀ **Camera**. !!! warning šŸ‘ Ensure the correct orientation ofĀ theĀ Ribbon CableĀ with the blue endĀ facing upwards. --- ![planktoscope-assembly-143.png](../images/assembly_guide/planktoscope-assembly-143.png) šŸ”“Ā Press down on the blackĀ connector on the Raspberry PiĀ camera board once the RibbonĀ Cable is in position. --- ![planktoscope-assembly-144.png](../images/assembly_guide/planktoscope-assembly-144.png) šŸ”“Ā **B1.**Small flat screwdriver 2mm --- ![planktoscope-assembly-145.png](../images/assembly_guide/planktoscope-assembly-145.png) - Now we will plug in theĀ **StepperĀ Mounts** to theĀ **HAT**. - šŸ”“The cables for theĀ **Stepper Mounts**Ā will be plugged into theĀ **HAT**Ā šŸŸ£here. !!! warning Hold tight, a specific order isĀ required. --- ![planktoscope-assembly-146.png](../images/assembly_guide/planktoscope-assembly-146.png) Starting with theĀ red cable, insert theĀ cable in the far left port and tightenĀ the screw situated above the port. !!! note The result should look the same asĀ the picture. --- ![planktoscope-assembly-147.png](../images/assembly_guide/planktoscope-assembly-147.png) Repeat this process with the orderĀ pictured here from left to right:Ā šŸ”“ RedĀ šŸŸ” YellowĀ šŸ”µ BlueĀ āš« Black. --- ## Chapter 17: Connect theĀ **LED**Ā andĀ **Peristaltic Pump**Ā to theĀ **Raspberry Pi** ![planktoscope-assembly-149.png](../images/assembly_guide/planktoscope-assembly-149.png) We will now be connecting the **LED**Ā and **Peristaltic Pump** with theĀ **Raspberry Pi**Ā **HAT**Ā andĀ **Camera**. --- ![planktoscope-assembly-150.png](../images/assembly_guide/planktoscope-assembly-150.png) Place the LED housing onto theĀ Stepper Mounts. !!! warning šŸ‘ Ensure correct orientation of bothĀ šŸŸ£ parts by looking at theĀ precut holes. --- ![planktoscope-assembly-151.png](../images/assembly_guide/planktoscope-assembly-151.png) !!! info The result should be similar to theĀ picture. --- ![planktoscope-assembly-152.png](../images/assembly_guide/planktoscope-assembly-152.png) Now we will plug in theĀ **LED** andĀ **Stepper Mount**Ā cables to theĀ **Raspberry Pi**. --- ![planktoscope-assembly-153.png](../images/assembly_guide/planktoscope-assembly-153.png) Feed the area of cabling that is notĀ covered by theĀ **spiral wrap**Ā throughĀ the two holes to start. Then threadĀ through to theĀ **spiral wrap**Ā so that itĀ matches the picture. --- ![planktoscope-assembly-154.png](../images/assembly_guide/planktoscope-assembly-154.png) We will now plug the cables into theĀ correct ports. --- ![planktoscope-assembly-155.png](../images/assembly_guide/planktoscope-assembly-155.png) šŸŸ£Ā The four wires (šŸ”“ RedĀ šŸ”µ Blue šŸŸ¢ Green āš« Black) enter the side port on theĀ Raspberry Pi HAT. --- ![planktoscope-assembly-156.png](../images/assembly_guide/planktoscope-assembly-156.png) šŸ”“ The two wires (LED) enter on theĀ port on top of the Raspberry Pi HAT. --- ![planktoscope-assembly-157.png](../images/assembly_guide/planktoscope-assembly-157.png) The result should be similar to theĀ picture. !!! note We will use this assembly in the nextĀ step. --- ## Chapter 18: Connect theĀ **DC Power Jack**Ā to theĀ **Raspberry Pi** ![planktoscope-assembly-159.png](../images/assembly_guide/planktoscope-assembly-159.png) - šŸŸ¢Ā Locate theĀ **DC Power Jack** - We will plug the DC Power JackĀ into the Raspberry Pi via the šŸ”µ blue port. --- ![planktoscope-assembly-160.png](../images/assembly_guide/planktoscope-assembly-160.png) šŸ”“Ā **B1.**Small flat screwdriver 2mm --- ![planktoscope-assembly-161.png](../images/assembly_guide/planktoscope-assembly-161.png) - Insert the šŸ”“ red cable of the **DCĀ Power Jack** into the **left side** of theĀ blue port. - Tighten the screw above. --- ![planktoscope-assembly-162.png](../images/assembly_guide/planktoscope-assembly-162.png) - Insert the āš« black cable of the **DCĀ PowerĀ Jack**Ā intoĀ theĀ **rightĀ side**Ā ofĀ theĀ blue port. - Tighten the screw above. --- ![planktoscope-assembly-163.png](../images/assembly_guide/planktoscope-assembly-163.png) !!! info The result should be similar to theĀ picture. !!! note We will use this assembly in the nextĀ step. --- ## Chapter 19: Your PlanktoScope starts to take shape ![planktoscope-assembly-165.png](../images/assembly_guide/planktoscope-assembly-165.png) --- ![planktoscope-assembly-166.png](../images/assembly_guide/planktoscope-assembly-166.png) Locate **Part I**Ā andĀ **Part H**. --- ![planktoscope-assembly-167.png](../images/assembly_guide/planktoscope-assembly-167.png) - SlotĀ **Part H**Ā intoĀ **Part I**. - šŸŸ£Ā Note the orientation.Ā **Part H**Ā goesĀ intoĀ **Part I**at the end with theĀ rectangular slot (as opposed to theĀ rectangle with bulbous hole). - šŸŸ”Ā Also, the deeper slots onĀ **part H**Ā should be on the upper side. --- ![planktoscope-assembly-168.png](../images/assembly_guide/planktoscope-assembly-168.png) šŸ”“ Slot theĀ **Peristaltic Pump**Ā aboveĀ theĀ **LED**. --- ![planktoscope-assembly-169.png](../images/assembly_guide/planktoscope-assembly-169.png) !!! note The result should be the same as theĀ picture. --- ![planktoscope-assembly-170.png](../images/assembly_guide/planktoscope-assembly-170.png) !!! warning šŸ‘ Ensure the correct orientation ofĀ the housing and theĀ **Peristaltic Pump**. --- ![planktoscope-assembly-171.png](../images/assembly_guide/planktoscope-assembly-171.png) At the other end, slot theĀ **DC PowerĀ Jack**Ā housing adjacent to theĀ **Raspberry Pi**. --- ![planktoscope-assembly-172-00.png](../images/assembly_guide/planktoscope-assembly-172-00.png) ![planktoscope-assembly-172-00.png](../images/assembly_guide/planktoscope-assembly-172-01.png) Rotate so that the DC Power Jack isĀ facing upwards. We will now slot the Raspberry Pi ontoĀ the rest of the housing. --- ![planktoscope-assembly-173.png](../images/assembly_guide/planktoscope-assembly-173.png) !!! info The result should be similar to thatĀ picture. !!! note šŸŽ¬Ā Store this assembly for later. --- ## Chapter 20: Inserting screws ![planktoscope-assembly-175.png](../images/assembly_guide/planktoscope-assembly-175.png) šŸŸ£ WeĀ willĀ nowĀ insertĀ eightĀ M3Ā screwsĀ to fasten the housing together. !!! note šŸŽ¬Ā Store this assembly for later. --- ![planktoscope-assembly-176.png](../images/assembly_guide/planktoscope-assembly-176.png) šŸ”“Ā **A6.** Screw M3X12mm BHC - SS --- ![planktoscope-assembly-177.png](../images/assembly_guide/planktoscope-assembly-177.png) šŸŸ”Ā **B3.** Allen key 2mm --- ![planktoscope-assembly-178.png](../images/assembly_guide/planktoscope-assembly-178.png) !!! info The result should be similar to theĀ picture. --- ![planktoscope-assembly-179.png](../images/assembly_guide/planktoscope-assembly-179.png) We will now turn over theĀ PlanktoScopeĀ andĀ repeatĀ theĀ processĀ for the underside. --- ![planktoscope-assembly-180.png](../images/assembly_guide/planktoscope-assembly-180.png) šŸŸ£Ā Insert eight more M3 screws on theĀ underside. --- ![planktoscope-assembly-181.png](../images/assembly_guide/planktoscope-assembly-181.png) !!! info The result should be similar to theĀ picture. --- ![planktoscope-assembly-182.png](../images/assembly_guide/planktoscope-assembly-182.png) Now turn the PlanktoScope on its side. --- ![planktoscope-assembly-183.png](../images/assembly_guide/planktoscope-assembly-183.png) --- ![planktoscope-assembly-184.png](../images/assembly_guide/planktoscope-assembly-184.png) !!! warning šŸ‘ Ensure the orientation of yourĀ PlanktoScope andĀ Part KĀ matchesĀ the picture. --- ![planktoscope-assembly-185.png](../images/assembly_guide/planktoscope-assembly-185.png) SlotĀ **Part K**Ā onto the rest of theĀ PlanktoScope. !!! warning šŸŸ¢Ā Ensure the correct orientation. TheĀ result should look the same at theĀ picture. --- ![planktoscope-assembly-186.png](../images/assembly_guide/planktoscope-assembly-186.png) Content ofĀ **Bag A**: šŸ”“Ā **A6.** Screw M3X12mm BHC - SS --- ![planktoscope-assembly-187.png](../images/assembly_guide/planktoscope-assembly-187.png) Content ofĀ **Bag B**: šŸŸ”Ā **B3.** Allen key 2mm --- ![planktoscope-assembly-188.png](../images/assembly_guide/planktoscope-assembly-188.png) šŸŸ£Ā Insert eight more M3 screws on theĀ side to holdĀ **Part K**Ā in place. --- ![planktoscope-assembly-189.png](../images/assembly_guide/planktoscope-assembly-189.png) --- ![planktoscope-assembly-190.png](../images/assembly_guide/planktoscope-assembly-190.png) We will now placeĀ **Part J**Ā into positionĀ as the housing for the side. !!! warning šŸ‘ Ensure your PlanktoScope matchesĀ the orientation in the picture. --- ![planktoscope-assembly-191.png](../images/assembly_guide/planktoscope-assembly-191.png) šŸŸ¢ PlaceĀ **Part K**Ā onto the rest of theĀ PlanktoScope and note the positionĀ of the cutout. --- ![planktoscope-assembly-192.png](../images/assembly_guide/planktoscope-assembly-192.png) Content ofĀ **Bag A**: šŸ”“Ā **A6.** Screw M3X12mm BHC - SS --- ![planktoscope-assembly-193.png](../images/assembly_guide/planktoscope-assembly-193.png) Content ofĀ **Bag B**: šŸŸ”Ā **B3.** Allen key 2mm --- ![planktoscope-assembly-194.png](../images/assembly_guide/planktoscope-assembly-194.png) šŸŸ£Ā Insert eight more M3 screws on theĀ underside --- ![planktoscope-assembly-195.png](../images/assembly_guide/planktoscope-assembly-195.png) !!! info The result should be similar to theĀ picture. --- ![planktoscope-assembly-196.png](../images/assembly_guide/planktoscope-assembly-196.png) --- ![planktoscope-assembly-197.png](../images/assembly_guide/planktoscope-assembly-197.png) Content ofĀ **Bag A**: šŸŸ”Ā **A5.** Screw M2.5X10mm CHC - SS --- ![planktoscope-assembly-198.png](../images/assembly_guide/planktoscope-assembly-198.png) PlaceĀ theĀ M2.5Ā screwĀ throughĀ **PartĀ N**. ItĀ willĀ actĀ asĀ aĀ **cover**Ā forĀ theĀ electricalĀ inputs. --- ![planktoscope-assembly-199.png](../images/assembly_guide/planktoscope-assembly-199.png) - Place theĀ **cover**Ā over the electricalĀ inputs on the PlanktoScope. - šŸŸ£ The screw will enter the holeĀ located here. --- ![planktoscope-assembly-200.png](../images/assembly_guide/planktoscope-assembly-200.png) Using the allen key, tighten the screwĀ so that it is possible to move theĀ cover with light force. --- ![planktoscope-assembly-201.png](../images/assembly_guide/planktoscope-assembly-201.png) !!! info TheĀ resultĀ shouldĀ beĀ theĀ sameĀ asĀ theĀ picture. --- ## Chapter 21: Insert the tubing in theĀ **Peristaltic Pump** ![planktoscope-assembly-203.png](../images/assembly_guide/planktoscope-assembly-203.png) - OrientateĀ yourĀ PlanktoScopeĀ soĀ thatĀ itĀ matches the picture. - Twist off the orange top of theĀ PeristalticĀ pumpĀ inĀ anĀ anti-clockwiseĀ direction. --- ![planktoscope-assembly-205.png](../images/assembly_guide/planktoscope-assembly-205.png) You can now remove the PeristalticĀ Pump housing andĀ šŸŸ¢ Rotor. --- ![planktoscope-assembly-206.png](../images/assembly_guide/planktoscope-assembly-206.png) You can now remove the PeristalticĀ Pump housing andĀ šŸŸ¢ Rotor. --- ![planktoscope-assembly-207.png](../images/assembly_guide/planktoscope-assembly-207.png) šŸ”“ Locate theĀ **Tube**Ā for the PeristalticĀ Pump inĀ **Bag F**Ā and remove it from theĀ bag. !!! warning The tips of the Tubing that areĀ covered by black rubber are veryĀ delicate and easily broken. --- ![planktoscope-assembly-208.png](../images/assembly_guide/planktoscope-assembly-208.png) Insert the first plastic arch of theĀ **Tube**Ā into the slot on the Peristaltic PumpĀ \ housing. !!! info The result should be similar to theĀ picture. --- ![planktoscope-assembly-209-00.png](../images/assembly_guide/planktoscope-assembly-209-00.png) ![planktoscope-assembly-209-01.png](../images/assembly_guide/planktoscope-assembly-209-01.png) Insert theĀ **Rotor**Ā into the housingĀ ensuring the correct orientation. TheĀ hole in the centre should be visible onĀ the underside. !!! info The result should be similar to theĀ picture. --- ![planktoscope-assembly-210.png](../images/assembly_guide/planktoscope-assembly-210.png) Insert theĀ **Rotor**Ā into the housing.Ā Then, thread theĀ **Tube**Ā around theĀ **Rotor**Ā and insert the other plasticĀ arch into the second slot. !!! info The result should be similar to theĀ picture. --- ![planktoscope-assembly-211.png](../images/assembly_guide/planktoscope-assembly-211.png) - Thread theĀ **Tube**Ā around around theĀ **Rotor**Ā and insert the other plasticĀ arch into the second slot. - This will require stretching theĀ **Tube**Ā slightly. --- ![planktoscope-assembly-212.png](../images/assembly_guide/planktoscope-assembly-212.png) !!! info The result should be similar to theĀ picture. --- ![planktoscope-assembly-213.png](../images/assembly_guide/planktoscope-assembly-213.png) Place the Peristaltic Pump housingĀ back onto the PlanktoScope. --- ![planktoscope-assembly-214.png](../images/assembly_guide/planktoscope-assembly-214.png) Achieve the angle shown in theĀ picture between the Peristaltic PumpĀ housing and PlanktoScope mainĀ body. Then, press and twist in aĀ clockwise direction. --- ![planktoscope-assembly-215.png](../images/assembly_guide/planktoscope-assembly-215.png) !!! info The result should be similar to theĀ picture. --- ![planktoscope-assembly-216.png](../images/assembly_guide/planktoscope-assembly-216.png) - šŸŸ£Ā Gently remove the black rubberĀ covers for the Peristaltic PumpĀ connectors by pinching the very tipĀ and pulling away. - Once complete, locateĀ **Bag D**Ā whichĀ contains tubing. --- ![planktoscope-assembly-217.png](../images/assembly_guide/planktoscope-assembly-217.png) Push the small piece of tubing fromĀ **Bag D**Ā over the left-side connector ofĀ theĀ **Peristaltic Pump**. --- ![planktoscope-assembly-218.png](../images/assembly_guide/planktoscope-assembly-218.png) Place the long piece of tubing fromĀ **Bag D**Ā over the right-side connectorĀ of theĀ **Peristaltic Pump**. --- ![planktoscope-assembly-219.png](../images/assembly_guide/planktoscope-assembly-219.png) Insert the connector fromĀ **Bag D**Ā intoĀ the other end of theĀ **small piece ofĀ tubing**. --- ![planktoscope-assembly-220.png](../images/assembly_guide/planktoscope-assembly-220.png) --- ![planktoscope-assembly-221.png](../images/assembly_guide/planktoscope-assembly-221.png) OrientateĀ **Part P**Ā so that the magnetsĀ are face-down on the left-hand side. --- ![planktoscope-assembly-222.png](../images/assembly_guide/planktoscope-assembly-222.png) Place **Part P** over the magnetsĀ adjacent to theĀ **Peristaltic Pump**. !!! info The result should be similar to theĀ picture. --- ![planktoscope-assembly-223.png](../images/assembly_guide/planktoscope-assembly-223.png) šŸ”“ FromĀ **Bag M**, Place theĀ **light blue**Ā **Test Tube** in the hole adjacent to theĀ Peristaltic Pump. --- ![planktoscope-assembly-224.png](../images/assembly_guide/planktoscope-assembly-224.png) Place theĀ šŸ”µ **dark blue Test Tube**Ā intoĀ the hole situated outside of theĀ PlanktoScope. --- ![planktoscope-assembly-225.png](../images/assembly_guide/planktoscope-assembly-225.png) šŸŸ¢Ā Insert the other end of the longĀ piece of tubing into theĀ šŸ”µ **dark blue** **Test Tube**. This will serve as the waste container.Ā The result should look the same asĀ the picture. --- ![planktoscope-assembly-226.png](../images/assembly_guide/planktoscope-assembly-226.png) --- ![planktoscope-assembly-227-00.png](../images/assembly_guide/planktoscope-assembly-227-00.png) ![planktoscope-assembly-227-01.png](../images/assembly_guide/planktoscope-assembly-227-01.png) ![planktoscope-assembly-227-02.png](../images/assembly_guide/planktoscope-assembly-227-02.png) - Locate PartĀ **C** - LocateĀ **25Ā mmĀ cameraĀ lens**Ā andĀ **Lock** **Ring**Ā fromĀ **Bag J**. - 25MM is printed on the lens. --- ![planktoscope-assembly-228-00.png](../images/assembly_guide/planktoscope-assembly-228-00.png) ![planktoscope-assembly-228-01.png](../images/assembly_guide/planktoscope-assembly-228-01.png) - Remove the plastic lens cap. - SlotĀ theĀ **25Ā mmĀ cameraĀ lens** intoĀ **Part C**. !!! info The magnets are raised on theĀ side where the lens lays flat. !!! warning Try not to touch the lens Screw theĀ **Lock Ring**Ā onto the lens,Ā **flat-side down**. --- ![planktoscope-assembly-229-00.png](../images/assembly_guide/planktoscope-assembly-229-00.png) ![planktoscope-assembly-229-01.png](../images/assembly_guide/planktoscope-assembly-229-01.png) ![planktoscope-assembly-227-02.png](../images/assembly_guide/planktoscope-assembly-227-02.png) - Locate PartĀ **D**. - LocateĀ **16Ā mmĀ cameraĀ lens**Ā andĀ **LockĀ Ring**Ā fromĀ **Bag J**. - 16MM is printed on the lens. --- ![planktoscope-assembly-230-00.png](../images/assembly_guide/planktoscope-assembly-230-00.png) ![planktoscope-assembly-230-01.png](../images/assembly_guide/planktoscope-assembly-230-01.png) - Remove the plastic lens cap. - SlotĀ theĀ **16Ā mmĀ cameraĀ lens** into **PartĀ D**. !!! info TheĀ magnetsĀ areĀ indentedĀ onĀ theĀ side that the lens lays flat. !!! warning Try not to touch the lens Screw theĀ **Lock Ring**Ā onto the lens,Ā **flat-side down**. The result should be similar to thatĀ picture. --- ![planktoscope-assembly-231-00.png](../images/assembly_guide/planktoscope-assembly-231-00.png) ![planktoscope-assembly-231-01.png](../images/assembly_guide/planktoscope-assembly-231-01.png) PlaceĀ bothĀ **Lenses** (**C**Ā andĀ **D**)Ā togetherĀ so that they flat together and bothĀ lenses are facing each other. !!! info The result should be similar to theĀ picture. --- ![planktoscope-assembly-232-01.png](../images/assembly_guide/planktoscope-assembly-232-01.png) ![planktoscope-assembly-232-00.png](../images/assembly_guide/planktoscope-assembly-232-00.png) ![planktoscope-assembly-232-02.png](../images/assembly_guide/planktoscope-assembly-232-02.png) PlaceĀ theĀ **Lenses**Ā (bothĀ **C**Ā andĀ **D**)Ā intoĀ position, adjacent to the camera. The orientation of your PlanktoScopeĀ and **Lenses** should match theĀ pictures. --- ![planktoscope-assembly-233.png](../images/assembly_guide/planktoscope-assembly-233.png) - OnceĀ together,Ā placeĀ theĀ lensesĀ (bothĀ **C** and **D**) into position, adjacent toĀ the camera. - PartĀ **C**Ā /Ā 25mmĀ lensĀ shouldĀ beĀ furthestĀ from the pump (orange piece). !!! info The result should be similar to theĀ picture. --- ![planktoscope-assembly-234.png](../images/assembly_guide/planktoscope-assembly-234.png) --- ![planktoscope-assembly-235.png](../images/assembly_guide/planktoscope-assembly-235.png) !!! info The Fluidic Path will have aĀ cardboard protector. Please take extra precaution while handlingĀ thisĀ partĀ andĀ avoidĀ touchingĀ the glass element of the piece. !!! warning The Fluidic Path is very delicate. --- ![planktoscope-assembly-236.png](../images/assembly_guide/planktoscope-assembly-236.png) Place the **Tube Clamp** over the longĀ pieceĀ ofĀ tubeĀ atĀ theĀ endĀ ofĀ theĀ **FluidicĀ Path**. --- ![planktoscope-assembly-237.png](../images/assembly_guide/planktoscope-assembly-237.png) Place the white clamp over the longĀ pieceĀ ofĀ tubeĀ atĀ theĀ endĀ ofĀ theĀ **Fluidic Path**. !!! info The result should look the same asĀ the picture. --- ![planktoscope-assembly-238.png](../images/assembly_guide/planktoscope-assembly-238.png) Press down on the **Tube Clamp** soĀ that it clicks into place with just oneĀ click. !!! info The result should look the same asĀ the picture. --- ![planktoscope-assembly-239.png](../images/assembly_guide/planktoscope-assembly-239.png) InsertĀ aĀ smallĀ plasticĀ **Connector**Ā intoĀ theĀ endĀ ofĀ theĀ tubing,Ā belowĀ theĀ **TubeĀ Clamp**. --- ![planktoscope-assembly-240.png](../images/assembly_guide/planktoscope-assembly-240.png) Make sure the tubing is over theĀ **Connector**. !!! info The result should look the same asĀ the picture. --- ![planktoscope-assembly-241.png](../images/assembly_guide/planktoscope-assembly-241.png) Remove the cardboard protectorĀ from theĀ **Fluidic Path**. --- ![planktoscope-assembly-242.png](../images/assembly_guide/planktoscope-assembly-242.png) !!! warning A reminder, the glass part of theĀ FluidicĀ PathĀ isĀ veryĀ delicate.Ā PleaseĀ tryĀ not to touch it. --- ![planktoscope-assembly-243.png](../images/assembly_guide/planktoscope-assembly-243.png) Place another **Connector** at the endĀ of the tubing. --- ![planktoscope-assembly-244.png](../images/assembly_guide/planktoscope-assembly-244.png) TheĀ resultĀ shouldĀ beĀ theĀ sameĀ asĀ theĀ picture. --- ![planktoscope-assembly-245.png](../images/assembly_guide/planktoscope-assembly-245.png) āš  Gently place the **Fluidic Path** inĀ the designated indentation onĀ **Part**Ā **F**. !!! warning Do not touch the glass.Ā  - You may have to slightly stretch theĀ tubing to get the Fluidic Path intoĀ position. - TheĀ resultĀ shouldĀ beĀ theĀ sameĀ asĀ theĀ picture. --- ![planktoscope-assembly-246.png](../images/assembly_guide/planktoscope-assembly-246.png) If you have tape available, place aĀ smallĀ pieceĀ atĀ theĀ theĀ bottomĀ ofĀ **Part F** so that the tubing remains fixed inĀ its position. Avoid placing tape over the glass. !!! info The result should be similar to theĀ picture. --- ![planktoscope-assembly-247-01.png](../images/assembly_guide/planktoscope-assembly-247-01.png) ![planktoscope-assembly-247-00.png](../images/assembly_guide/planktoscope-assembly-247-00.png) ToĀ insertĀ theĀ syringe,Ā placeĀ fingerĀ andĀ thumb either side of part **F** whereĀ green circle is located. This ensuresĀ the tubing does not rotate while youĀ twist the syringe into position in aĀ clockwise direction. --- ![planktoscope-assembly-248.png](../images/assembly_guide/planktoscope-assembly-248.png) !!! info The result should be similar to theĀ picture. --- ![planktoscope-assembly-249-00.png](../images/assembly_guide/planktoscope-assembly-249-00.png) ![planktoscope-assembly-249-01.png](../images/assembly_guide/planktoscope-assembly-249-01.png) - Place the **Fluidic Path** into positionĀ between the mount and theĀ Peristaltic Pump. - Connect the magnets to face eachĀ other. --- ![planktoscope-assembly-250.png](../images/assembly_guide/planktoscope-assembly-250.png) The result should look the same asĀ the pictures. --- ![planktoscope-assembly-251-00.png](../images/assembly_guide/planktoscope-assembly-251-00.png) ![planktoscope-assembly-251-01.png](../images/assembly_guide/planktoscope-assembly-251-01.png) - šŸŸ¢ Connect the **Fluidic Path** to theĀ **Peristaltic Pump** by twisting the twoĀ connectors together. - TheĀ resultĀ shouldĀ beĀ theĀ sameĀ asĀ theĀ picture. --- ## Build complete! šŸ’Æ šŸ’« The next stage is to plug in our PlanktoScopeĀ and connect it to the computer.