Open and Affordable Quantitative Imaging Platform https://www.planktoscope.org
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PlanktonScope

This GitHub is part of a community that you can find on this website : https://www.planktonscope.org/

Basic Installation

Before going further, notice that you can download the image disk already setup without having to deal with all these command lines. Jump here : http://planktonscope.su.domains/Images_raspberry/Raspbian_Buster_Morphocut_WiFi.img

Expert Installation


Install Raspbian on your Raspberry Pi


Download the image

Download the .zip file of Raspbian Buster with desktop from the Raspberry Pi website Downloads page.

Writing an image to the SD card

Download the latest version of balenaEtcher and install it.

Connect an SD card reader with the micro SD card inside.

Open balenaEtcher and select from your hard drive the Raspberry Pi .zip file you wish to write to the SD card.

Select the SD card you wish to write your image to.

Review your selections and click 'Flash!' to begin writing data to the SD card.

Prepare your Raspberry Pi

Getting Started with your Raspberry Pi <https://projects.raspberrypi.org/en/projects/raspberry-pi-getting-started/>_

Plug the SD Card in your Raspberry Pi

Connect your Pi to a screen, mouse, keyboard and power

Finish the setup

Make sure you have access to internet and update/upgrade your fresh raspbian

Update your Pi first :: sudo apt-get update -y sudo apt-get upgrade -y

Reboot your Pi safely :: sudo reboot now


Raspberry Pi configurations


Enable Camera/SSH/I2C in raspi-config

Open up the configuration page and select Interfacing Options by typing this command: :: sudo raspi-config

Select Serial

Select NO

Keep the Serial Port Hardware enabled

Reboot your Pi safely :: sudo reboot now


Install the needed libraries for the PlanktonScope


Install CircuitPython

Installing CircuitPython on Raspberry Pi <https://learn.adafruit.com/circuitpython-on-raspberrypi-linux/installing-circuitpython-on-raspberry-pi>_

Run the following command to install adafruit_blinka :: pip3 install adafruit-blinka sudo pip3 install adafruit-circuitpython-motorkit

Install RPi Cam Web Interface

RPi Cam Web Interface <https://elinux.org/RPi-Cam-Web-Interface>_

Clone the code from github and enable and run the install script with the following commands :: git clone https://github.com/silvanmelchior/RPi_Cam_Web_Interface.git cd RPi_Cam_Web_Interface ./install.sh

Press Enter to allow default setting of the installation Press Enter to start RPi Cam Web Interface now Found what is the IP of your Raspberry Pi :: sudo ip addr show | grep 'inet 1'

Reach the url on a local browser : http://127.0.0.1/html/

Install Ultimate GPS HAT

Installing Adafruit GPS HAT <https://learn.adafruit.com/adafruit-ultimate-gps-hat-for-raspberry-pi/pi-setup>_

Use Python Thread with GPS HAT <http://www.danmandle.com/blog/getting-gpsd-to-work-with-python/>_

:: sudo apt-get install python gpsd gpsd-clients

Install RGB Cooling HAT

Installing RGB Cooling HAT <https://www.yahboom.net/study/RGB_Cooling_HAT>_ Type these command to install: :: git clone https://github.com/WiringPi/WiringPi.git cd WiringPi sudo ./build sudo apt-get install gcc

Install Node-RED

Installing Node-RED on Raspberry Pi <https://nodered.org/docs/getting-started/raspberrypi>_

Prerequisites

Ensure npm is able to build any binary modules it needs to install. :: sudo apt-get install build-essential

Download and installation

To install Node.js, npm and Node-RED onto a Raspberry Pi, run the following command will that download and install them: :: bash <(curl -sL https://raw.githubusercontent.com/node-red/linux-installers/master/deb/update-nodejs-and-nodered)

Due to the limited memory of the Raspberry Pi, you will need to start Node-RED with an additional argument to tell the underlying Node.js process to free up unused memory sooner than it would otherwise. :: node-red-pi --max-old-space-size=256

Autostart on boot

Run Node-RED when the Pi is turned on, or re-booted, enable the service to autostart by running the command: :: sudo systemctl enable nodered.service

Check the installation

Make sure NodeRed is correctly installed by reaching the following page from the broswer of your pi : :: http://localhost:1880.

Install few nodes

These nodes will be used in Node-RED: ::
cd .node-red/ npm install node-red-dashboard npm install node-red-contrib-python3-function npm install node-red-contrib-camerapi npm install node-red-contrib-gpsd npm install node-red-contrib-web-worldmap

Import the last GUI

Import the lastest version of the GUI <https://raw.githubusercontent.com/tpollina/PlanktonScope/master/scripts/flows_planktonscope.json>_

Install Mosquitto MQTT

In order to send and receive from Node-RED: :: sudo apt-get install mosquitto mosquitto-clients

Install mqtt-paho

In order to send and receive from python: :: pip3 install paho-mqtt

Install OpenCV

Use the quick version without virtual env https://www.pyimagesearch.com/2019/09/16/install-opencv-4-on-raspberry-pi-4-and-raspbian-buster/

Install MorphoCut

Installing MorphoCut

MorphoCut is packaged on PyPI and can be installed with pip:

sudo apt-get install python3-scipy
pip3 install -U git+https://github.com/morphocut/morphocut.git

Finishing the install

Make sure to update your Pi :: sudo apt-get update -y sudo apt-get full-upgrade -y

Reboot your Pi safely :: sudo reboot now


Usefull later maybe


Download the GitHub repo

At this link : https://github.com/tpollina/PlanktonScope/archive/master.zip Unzip to a specific location: :: unzip /home/pi/Downloads/PlanktonScope-master.zip -d /home/pi/ mv /home/pi/PlanktonScope-master /home/pi/PlanktonScope

Update node-RED interface

To update the interface, you can just download the lastest .json file: :: wget -P $HOME/.node-red https://raw.githubusercontent.com/tpollina/PlanktonScope/master/scripts/flows_planktonscope.json

Share WiFi via Ethernet

At this link : https://www.instructables.com/id/Share-WiFi-With-Ethernet-Port-on-a-Raspberry-Pi/