61 lines
1.8 KiB
Markdown
61 lines
1.8 KiB
Markdown
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# openSMAPPjs
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My little SMAPP should be based on javascript and work without a backend server. The backend is only used to persist data and send it to the lab.
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It should be possible to use the app as is - without the backend side.
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For this data is stored in the local-storage of the browser.
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## Configure The App
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+ create the database
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### Sample Preparation Protocol
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+ settling time (default 15 s)
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+ dilution (main default 1:5 and bacterial default 1:500)
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+ sample Volume (1 mL)
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+ shaking time (30x shakes in 30s)
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### Microscope Camera Setup
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+ width / height of FoV [um]
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+ objective magnification (default 40x)
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+ eypiece Number
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+ size of slide width x height
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## Sample Intake Form
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+ type (compost)
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+ place
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+ date + time of taking the sample
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+ notes (when was the last rain event)
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## Sample Preparation
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After the microscope and the preparation protocol is defined we can start taking in samples.
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### Sample Preparation
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+ dilution (main 1:5)
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+ shaking time (30x shakes in 30s)
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+ settling time (15 s)
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### Slide Preparation
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+ size of slide
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+ sample size [uL]
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## Sample Analysis
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The analysis consists of a Nematode scan the main assessment for protozoa and filamentous organisms and a bacteria count.
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### Nematode Scan
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Take multiple pictures of each Nematode and calssify to functional group.
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Bacterial- / Fungal- / Rootfeeding and Predatory Nematodes.
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### Main Assessment
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Take multiple pictures per FoV and count all organisms in the FoV. Mark them and store the picture and list of organisms.
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+ as many as needed pictures per FoV.
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+ 5 or more FoV per reading.
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+ 5 or more readings.
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### Bacterial Count
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For the bacterial count another dilution is used. Then bacteria are counted.
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## Results Page
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From the different parts of the analysis the F:B ratio is calculated and Number of Protozoa / Fungal and Bacterial Biomass per mL of soil is calculated.
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