Filamentous Organisms and Protozoa
The main scan was performed on ??? using the
protocol ??? and camera setup
???.
Dividing the sample volume VSample = ??? uL
by the size of the coverslip Acoverslip =
??? mm x ??? mm
yields the observed height hobserved = VSample / Acoverslip =
um. Multipilcation with the size of the the field of view
AFoV = ??? um x
??? um
yields the observed Volume Vobserved =
hobserved * AFoV = ??? uL per FoV.
The volume of organisms per FoV is multiplied with the
dilution Factor Fdilution = ???
and devided by Vobserved to calculate the abundance per uL.
Fmultiplication = Fdilution * 1 uL / Vobserved = ???
Filamentous Organisms and Protozoa Table
|
organism
|
abundance [ug/uL] or [1/uL]
|
standard deviation [%]
|
Bacteria Scan
The bacteria scan has been performed on ???.
Dividing the sample volume VSample = ??? uL
by the size of the coverslip Acoverslip =
??? mm x ??? mm
yields the observed height hobserved = VSample / Acoverslip =
um. Multipilcation with the size of the
the field of view AFoV = ??? um x
??? um yields the observed Volume
Vobserved = hobserved * AFoV = ??? uL per FoV. The volume of organisms per FoV is multiplied with the
dilution Factor Fdilution = ???
and devided by Vobserved to calculate the abundance per uL:
Fmultiplication = Fdilution * 1 uL / Vobserved = ???
Bacteria Table
|
type of Bacteria
|
count per FoV
|
area [um²] per FoV
|
number per uL +- [%]
|
~ ug / uL +- [%]
|
Bacterial to Fungal Biomass
The Bacterial mBacteria = ???
to Fungal Biomass mFungi = ???.
mFungi / mBacteria = ???
Nematode Scan
The namatode scan has been performed on ???
using the protocol ??? and camera setup
???.
To calculate the abundance of nematodes per cubic centimeter of sample the dilution Factor Fdilution =
??? is divided by the sample size Vsample =
??? uL.
The multiplication factor for the nematode scan is:
Fnematode = Fdilution / Vsample = ???
Nematode Table
|
functional group
|
count
|
number per cm³
|