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planktoscope/docs/hardware/assembly_guide.md

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Assembly guide of the PlanktoScope

Content of the Kit

planktoscope-assembly-002.png

It is important to ensure that you have all of the necessary components before beginning the assembly of your PlanktoScope. To do so, please check that all bags are present as part of the kit.

Bag Content
A Scews
B Tools
C Adhesive Pads
D Tubing, Glass Cuvettes
E Bubbler Pump
F Peristaltic Pump
G Linear Stepper Motor
H Raspberry PI Chip cooler
I Raspberry HAT
J Camera Lens
K MicroSD Card, DC Power Jack Socket
L DC Power Supply and Cable
M Syringe and Falcon Tube
X1 Raspberry PI 4
X2 Pipet
X3 Cable ties
X4 Raspberry PI HQ Camera Modul
X5 Sandpaper

If any bags are missing, please go back to the BOM (Bill of Materials) and reorder the required components.

About this document

To read this document, follow these guidelines:

Color codes

  • šŸ”“Ā Look to the color red
  • šŸŸ Ā Look to the color orange
  • šŸŸ”Ā Look to the color yellow
  • šŸŸ¢Ā Look to the color green
  • šŸ”µĀ Look to the color blue
  • šŸŸ£Ā Look to the color purple

Icons

  • šŸ‘Ā Pay attention to this
  • āš Ā Be careful with this
  • šŸ“œĀ The book says
  • šŸŽ¬Ā Action !
  • āŒĀ Don't focus on that location

As you read through the document, be sure to pay attention to these visual cues to guide you through the build process.

Requirements

Tools

planktoscope-assembly-254.png

Content ofĀ Bag B:

  • šŸ”“Ā B1. Small flat screwdriver 2mm
  • šŸŸ Ā B2. Razor blade
  • šŸŸ”Ā B3. Allen key 2mm
  • šŸŸ¢ B4. Wrenches for standoffs

Components

planktoscope-assembly-253.png

Content ofĀ Bag A:

  • šŸŸ¢Ā A1. Standoff M2.5 - 6mm - Brass
  • šŸ”µĀ A2. Standoff M2.5 - 15mm - Ā Brass
  • šŸŸ£Ā A3. Standoff M2.5 - 16mm - SS
  • šŸŸ Ā A4. Screw M2.5x5mm CHC - SS
  • šŸŸ”Ā A5. Screw M2.5x10mm CHC - SS
  • šŸ”“Ā A6. Screw M3x12mm BHC - SS

Chapter 1: Detach the Parts from panels by cutting the tabs

planktoscope-assembly-004.png šŸ‘ Locate the panel S1 and discover the 5 differents Parts F, P, K, J and I.

planktoscope-assembly-005.png šŸŽ¬ Flip your panel S1.

planktoscope-assembly-006.png šŸ”“ Locate the outer tabs on the edges of the different Parts. These are typically small projections of material that are used to secure the case parts to the panels.

planktoscope-assembly-007.png Gather all the necessary tools. You will need the B2 šŸŸ  Razor blade to cut the tabs.

planktoscope-assembly-008.png

  • šŸŸ£ Use the razor blade to cut the outer tabs located on the edges of the different Parts
  • āŒ Do not cut the inner tabs present inside the different Parts for now and focus on the outer tabs attaching the Parts to the main panel.

planktoscope-assembly-009.png

  • Position your razor blade on the tab as close to the piece as possible to avoid residual tab after cutting.
  • Press firmly on the razor blade, being very careful with your finger, to cut your first tab.
  • Make sure you don't damage your table by placing a flat, rigid support under the S1 panel.
  • Keep going with the other tabs of this piece F.

Once all of the tabs are cut, gently lift the case parts away from the panels. If the case parts are stuck or difficult to remove, you may need to gently wiggle or pry them loose using a flat tool such as a screwdriver.

!!! warning

Be extremely careful because this is very sharp.

planktoscope-assembly-011.png

Once you have removed your Part from the main panel by cutting off all the tabs holding it, inspect it for potential residual tabs.

  • šŸŸ£ Here is a residual tab that will need to be removed.
  • šŸŸ  Here there is no residual tab which is perfect.

planktoscope-assembly-012.png

šŸŸ£ Place your razor blade flat on the edge of your piece being very careful with your fingers and cut the residual tab.

!!! warning

Be extremely careful because this is very sharp.

planktoscope-assembly-013.png

Repeat the cutting of the tabs on all the Parts F, P, K, J and I present on the panel S1.

!!! warning

Be extremely careful because this is very sharp.

planktoscope-assembly-014.png

šŸ”“ Locate the inner tabs on the edges of the different Parts.

planktoscope-assembly-015.png

Cut out the tabs inside of all the Parts F, P, K, J and I detached from the panel S1.

Dispose of the cut tabs and any other debris that may have been created during the detachment process.

!!! warning

Be extremely careful because this is very sharp.

planktoscope-assembly-016.png

  • āœ… Good way of cutting inner tabs
  • āŒ Wrong way of cutting inner tabs

planktoscope-assembly-018.png

Repeat the process on the panel S2.

!!! warning

Be extremely careful because this is very sharp.

planktoscope-assembly-019.png

Discover the 11 differents Parts.

planktoscope-assembly-020.png

Dispose of the cut tabs and any other debris that may have been created during the detachment process. Inspect the case parts and panels for any damage or imperfections that may have occurred during the detachment process. If any damage is found, it may be necessary to repair or replace the affected parts.

Chapter 2: Place the 4 Adhesive Pads under the Part I

planktoscope-assembly-023.png To secure the PlanktoScope on slippery grounds using the adhesive pads, follow these steps. Gather all the necessary materials. You will need:

  • Time: 1 min
  • šŸ‘ and Take the Part I
  • šŸŸ  Take the four adhesive pads present in the bag A.
  • šŸŸ£ Locate the four pockets that will receive the four adhesive pads.

planktoscope-assembly-024.png

  • Clean the bottom of the case part I. Make sure the surface is free of dirt, debris, and any other substances that may prevent the adhesive pads from sticking properly.
  • Remove the paper and place the four adhesive pads in the pockets by pressing firmly on them, sticky-side down.
  • Test the stability of the PlanktoScope by gently shaking or tilting it. If it feels secure and does not slip or slide, the adhesive pads have been successfully installed.

!!! note

šŸŽ¬ Store this assembly for later.

Chapter 3: Screw the four Standoffs into Part A

planktoscope-assembly-026.png

Now it's time to assemble the ground plate for the Raspberry Pi as the PlanktoScope main processing unit.

  • Time: 5 min

planktoscope-assembly-027.png

  • šŸ‘ Grab the Part A.
  • šŸŸ£ Locate the four holes on Part A.

planktoscope-assembly-028.png

šŸŸ¢ A1. Standoff M2.5 - 6mm- Brass

planktoscope-assembly-029.png

šŸŸ¢ B4. Wrenches for standoffs

planktoscope-assembly-030.png

  • šŸŸ£ Place the Standoff M2.5 - 6mm in the small side of the wrenches for standoffs B4.
  • šŸŸ  Do not use the big side of the wrenches for standoffs since the standoff will be loose in it.

planktoscope-assembly-031.png

  • Place the standoff in the hole and start rotating by hand in a clockwise direction until secure.
  • Then tighten with the wrench.

planktoscope-assembly-032.png

  • āœ… Make sure to screw until the standoff is properly inserted in the hole.
  • āŒ Do not stop screwing before.

planktoscope-assembly-033.png

Keep going for each of the four holes.

Chapter 4: Mount the Heat Sinks on the Raspberry Pi

planktoscope-assembly-035.png

  • Time: 2 min

Locate the Raspberry Pi 4 Model B packaging.

!!! warning

Be careful removing it from its packaging.

planktoscope-assembly-036.png

Place the four Heat Sinks next to your Raspberry Pi and mark the locations of the Heat Sinks on the Raspberry Pi.

  • šŸŸ  & šŸ”µ Small Heat Sinks
  • šŸŸ¢ Medium Heat Sink
  • šŸŸ£ Big Heat Sink

planktoscope-assembly-037.png

Remove the protective labels under a Heat Sink and place the Heat Sink on the slot of the Raspberry Pi.

planktoscope-assembly-038.png

Remove the protective labels under all the Heat Sinks and place all the Heat Sinks on the slots of the Raspberry Pi.

Chapter 5: Insert the micro SD card in the Raspberry Pi

planktoscope-assembly-040.png

  • Locate the SD card adapter in the bag K.
  • The micro SD card is inserted in the SD card adapter.
  • šŸŸ£ Remove the micro SD card from the SD card adapter.

planktoscope-assembly-041.png

  • Flip your Raspberry Pi.
  • šŸŸ  Locate the micro SD port.
  • šŸŸ£ Insert the micro SD card in the Raspberry Pi.

planktoscope-assembly-042.png

Push the micro SD card in the Raspberry Pi port to a point of resistance.

!!! note

If you notice that the **micro SD card** protrudes about 2mm from its slot, this is normal.

Chapter 6: Mount the Raspberry Pi on the Part A

  • Time: 1 min

planktoscope-assembly-044.png

  • āœ… Make sure to position the Raspberry Pi properly on the four standoffs screwed on the Part A.
  • āŒ Do not invert the position of the Raspberry Pi on the four standoffs screwed on the Part A.

planktoscope-assembly-045.png

šŸŸ£ A3. Standoff M2.5 - 16mm - SS

planktoscope-assembly-046.png

Screw by hand a Standoff M2.5 - 16mm on the Raspberry Pi.

planktoscope-assembly-047.png

  • Screw by hand all Standoffs M2.5 - 16mm on the Raspberry Pi.
  • Make sure you insert all four standoffs by hand and tighten slightly.

planktoscope-assembly-048.png

šŸŸ¢ B4. Wrenches for standoffs

planktoscope-assembly-049.png

  • šŸŸ  Secure the Standoff M2.5 - 16 mm - SS A3 in the big side of the wrenches for standoffs B4.
  • šŸŸ£ Do not use the small side of the wrenches for standoffs since the standoff wonā€™t fit in it.

Chapter 7: Attach the Ribbon Cable to the Raspberry Pi

  • Time: 2 min

planktoscope-assembly-051.png

Locate the Raspberry Pi Camera HQ packaging.

!!! warning

Be careful removing it from its packaging.

planktoscope-assembly-052.png

Lay your Raspberry Pi Camera face down on a suitable surface.

šŸ”“ The black connector is simply a push/pull fit. To disengage the cable, pull the two corners of the black connector down, away from the camera board. It will unclip to about 3mm, make sure you don't pull it off! If you're struggling, try pulling off one corner of the connector at a time.

!!! warning

Be careful with this, this part is delicate. Lift the black connector gently

planktoscope-assembly-053.png

Once the connector has been disengaged from the Raspberry Pi camera board, the cable will simply slide out!

  • šŸŸ£ Put aside Camera the Raspberry Pi
  • šŸŸ¢ Keep the Ribbon Cable for next step.

planktoscope-assembly-054.png

šŸ”“ Locate the black connector present on the Raspberry Pi.

planktoscope-assembly-055.png

šŸ”“ The black connector is simply a push/pull fit. To disengage the cable, pull the two corners of the black connector down, away from the camera board. It will unclip to about 3mm, make sure you don't pull it off! If you're struggling, try pulling off one corner of the connector at a time.

!!! warning

Be careful, this part is delicate. Gently prise the black connector with nail or fingertip and thumb.

planktoscope-assembly-056.png

Insert the Ribbon Cable you just detached from the Raspberry Pi Camera in the Raspberry Pi.

  • Make sure to insert in as much as you can.
  • Blue rectangle on Ribbon Cable should face the same direction as the arrow below.

planktoscope-assembly-057.png

šŸ”“ Secure the Ribbon Cable in the Raspberry Pi by pressing firmly on the black connector.

Chapter 8: Mount the PlanktoScope HAT on the Raspberry Pi

planktoscope-assembly-059.png

  • Time: 2 min

Locate the PlanktoScope HAT present in bag I.

planktoscope-assembly-060.png

šŸ”“ Thread the Ribbon cable through the PlanktoScope HAT slot from the underside.

!!! warning

Make sure the two šŸŸ£ black connectors are aligned before threading through the ribbon.

planktoscope-assembly-061.png

šŸ”“ Plug the PlanktoScope HAT into the Raspberry Pi.

!!! warning

Make sure the two black connectors are aligned before attaching them together.

planktoscope-assembly-062.png

Press the PlanktoScope HAT against the Raspberry Pi until it is no longer possible to move them closer together.

!!! warning

Continue to feed through the Ribbon Cable and do not crush it while pressing the PlanktoScope HAT against the standoffs.

planktoscope-assembly-063.png

šŸŸ  A4. Screw M2.5X5mm CHC - SS

planktoscope-assembly-064.png

šŸŸ£ Locate the 4 holes on the top of the PlanktoScope HAT and insert the four M2.5X5mm

planktoscope-assembly-065.png

šŸŸ” B3. Allen key 2mm

planktoscope-assembly-066.png

Screw the four A4 screws through the PlanktoScope HAT onto the Standoff M2.5 - 16mm.

!!! note

šŸŽ¬ Store this assembly for later.

Chapter 9: Place the Power Socket on Part M

planktoscope-assembly-068.png

  • Time: 2 min
  • Locate the DC Power Jack from the Bag K.
  • Remove the Lock Ring from the DC Power Jack

planktoscope-assembly-069.png

planktoscope-assembly-070.png

  • šŸ”“ Lay the Part M down and make sure the pockets in these holes are facing upwards.
  • šŸŸ£ Locate the Power Socket hole on Part M.

planktoscope-assembly-071.png

šŸ”“ Insert the cable inside of the hole by being sure of the orientation of the Part M.

planktoscope-assembly-072.png

šŸŸ£ Flip the Part M and secure the DC Power Jack by hand on the Part M by screwing the Lock Ring.

!!! warning

Make sure the Lock Ring doesnā€™t spin on itself.

!!! note

šŸŽ¬ Store this assembly for later.

Chapter 10: Mount the Raspberry Pi Camera HQ on Part B

planktoscope-assembly-074.png

  • Time: 2 min

planktoscope-assembly-075.png

šŸŸ£ Locate the 4 holes on the top of the Part B.

planktoscope-assembly-076.png

šŸ”µ A2. Standoff M2.5 - 15mm - Brass

planktoscope-assembly-077.png

Insert the four Standoff M2.5 - 15mm.

planktoscope-assembly-078.png

The result should be similar to the picture.

planktoscope-assembly-079.png

šŸŸ¢ B4. Wrenches for standoffs

planktoscope-assembly-080.png

Using the small side of the Standoff Wrench, secure the 4 M2.5 - 15mm Standoffs

planktoscope-assembly-081.png

  • āœ… Make sure to screw until the Standoff is properly tightened into the hole.
  • āŒ Do not stop screwing before.

planktoscope-assembly-082.png

Locate the Raspberry Pi Camera HQ

planktoscope-assembly-083.png

Remove the lens cap Raspberry Pi Camera HQ.

planktoscope-assembly-084.png

!!! warning

Make sure your camera lens is clean. If it is not, gently wipe using cotton swab for this task.

planktoscope-assembly-085.png

Place the Raspberry Pi Camera HQ on top of the four Standoffs installed on Part B.

šŸŸ£Ensure correct orientation of the Raspberry Pi Camera HQ. The black connector where the Ribbon Cable was removed is on the same side as the šŸŸ¢slot circled in green

planktoscope-assembly-086.png

šŸŸ  A4. Screw M2.5X5mm CHC - SS

planktoscope-assembly-087.png

šŸŸ” B3. Allen key 2mm

planktoscope-assembly-088.png

Use the allen key and tighten the Raspberry Pi Camera to the Standoffs.

planktoscope-assembly-089.png

The result should be similar to the picture.

!!! note

šŸŽ¬ Store this assembly for later.

Chapter 11: Mount the Linear Stepper Motor on Part E

planktoscope-assembly-091.png

Locate the Stepper Motors

!!! warning

Avoid touching the metal rods on the Stepper Motors

!!! info

You can touch the šŸŸ£ gold stands

planktoscope-assembly-092.png

planktoscope-assembly-093.png

šŸŸ” A5. Screw M2.5X10mm CHC - SS

planktoscope-assembly-094.png

šŸŸ” B3. Allen key 2mm

planktoscope-assembly-095.png

  • šŸ”“ Lay the Part E down and make sure the pockets in these holes are facing upwards.
  • šŸŸ£ Locate the four holes on Part E and place four M2 Screws in the holes.

planktoscope-assembly-096.png

Attach the stepper motors to the screws we have just placed with the šŸ”“ pockets positioned on opposite to the cabling.

The result should be similar to the picture.

planktoscope-assembly-097.png

Use the 2mm allen key to fix the Stepper Motors.

The result should be similar to that picture.

planktoscope-assembly-098.png

The result should be similar to the picture.

planktoscope-assembly-099.png

Repeat the process on the other side with the other Stepper Motor.

planktoscope-assembly-100.png

Repeat the process on the other side with the other Stepper Motor.

planktoscope-assembly-101.png

The result should be similar to the picture.

!!! note

šŸŽ¬ Store this assembly for later.

Chapter 12: Mount theĀ LEDĀ onĀ Part G

planktoscope-assembly-103.png

  • Locate theĀ LEDĀ andĀ LED cableĀ in Bag K.
  • šŸŸ£Ā The LED will go on the end whereĀ the white plastic connector isĀ smallest.

planktoscope-assembly-104.png

Insert theĀ LED into the LED cable.

planktoscope-assembly-105.png

The result should be similar to theĀ picture.

planktoscope-assembly-106.png

planktoscope-assembly-107.png

Locate partĀ G.

planktoscope-assembly-108.png

šŸŸ£Ā Locate the LED hole onĀ Part G.

planktoscope-assembly-109.png

WeĀ willĀ nowĀ placeĀ theĀ LEDĀ intoĀ theĀ slotĀ on partĀ G.

planktoscope-assembly-110.png

!!! warning

Gently push the LED into the LEDĀ hole located on PartĀ G. It should be a snug fit.

planktoscope-assembly-111.png

The result should be similar to theĀ picture.

!!! info

šŸŽ¬Ā Store this assembly for later.

Chapter 13: Mount theĀ Peristaltic PumpĀ onĀ Part O andĀ Part L

planktoscope-assembly-113.png

  • Locate theĀ Kamoer Peristaltic pumpĀ from theĀ Bag F.
  • šŸŸ£Ā Put aside theĀ tubing contained inĀ the little bag.

planktoscope-assembly-114.png

planktoscope-assembly-115.png

šŸŸ¢Ā Insert the cable of theĀ Peristaltic PumpĀ into the hole onĀ Part OĀ andĀ then insert the motor block assemblyĀ of the pump into it.

!!! warning

šŸ‘ Ensure the correct orientation ofĀ Part OĀ and theĀ Peristaltic Pump

planktoscope-assembly-116.png

šŸŸ”Ā A5. ScrewĀ M2.5X10mmĀ CHC - SS

planktoscope-assembly-117.png

šŸŸ”Ā B3. Allen key 2mm B3

planktoscope-assembly-118.png

šŸŸ¢Ā Insert the twoĀ M2.5X10mmĀ in theĀ two holes.

planktoscope-assembly-119.png

Screw the twoĀ M2.5X10mmĀ into theĀ two holes.

planktoscope-assembly-120.png

planktoscope-assembly-121.png

šŸ”“Ā LayĀ theĀ PartĀ LĀ down and makeĀ sure the pockets in these holes areĀ facing upwards.

planktoscope-assembly-122.png

  • PlaceĀ theĀ Peristaltic Pump underneath part L, ensuring theĀ correct orientation of these two parts.
  • šŸ”“ Insert the Peristaltic Pump intoĀ the allocated slot inĀ Part L.

planktoscope-assembly-123.png

Insert theĀ Peristaltic PumpĀ into theĀ allocated slot inĀ Part L.

planktoscope-assembly-124.png

Insert theĀ Peristaltic PumpĀ into theĀ allocated slot inĀ Part L.

planktoscope-assembly-125.png

  • Lay the assembly down.
  • šŸ”“Ā Locate theĀ four different holes.

planktoscope-assembly-126.png

šŸŸ”Ā **A5.**ScrewĀ M2.5X10mmĀ CHC - SS

planktoscope-assembly-127.png

šŸŸ”Ā **B3.**Allen key 2mm

planktoscope-assembly-128.png

Screw the four M2.5X10mm in theĀ locatedĀ holesĀ attachingĀ theĀ PartĀ OĀ toĀ theĀ Part L Peristaltic Pump.

planktoscope-assembly-129.png The result should be similar to theĀ picture.

!!! info

We will use this part in the next step.

Chapter 14: Spiral wrap the LED and Peristaltic Pump cabling

planktoscope-assembly-131.png

  • Locate the LED and housing, alongĀ with the pump and housing.
  • šŸŸ£Ā Locate theĀ spiral wrapĀ fromĀ bag K.

planktoscope-assembly-132.png

  • Spiral wrap both sets of cablesĀ together.
  • There should be 4 cm (1.5 inches)Ā betweenĀ theĀ connectorsĀ andĀ theĀ startĀ of the spiral wrap.

planktoscope-assembly-133.png

Continue wrapping around theĀ cables until you have used all of theĀ spiral wrap, leaving small or no gaps.

!!! info

The result should look the same asĀ the picture.

planktoscope-assembly-134.png

!!! info

The result should look the same asĀ the picture.

!!! note

šŸŽ¬Ā Store this assembly for later.

Chapter 15: Attaching theĀ Stepper MotorsĀ to theĀ Raspberry Pi Camera

planktoscope-assembly-136.png

Locate the Stepper Motors withĀ mount, and the Raspberry PiĀ Camera.

planktoscope-assembly-137.png Feed the Stepper Motor cables intoĀ theĀ slotsĀ eitherĀ sideĀ ofĀ theĀ RaspberryĀ Pi Camera.

!!! warning

Make sure the orientation isĀ correct and matches the picture.

planktoscope-assembly-138.png Feed the Stepper Motor cables intoĀ theĀ slotsĀ eitherĀ sideĀ ofĀ theĀ RaspberryĀ Pi Camera.

!!! warning

Make sure the orientation isĀ correct and matches the picture.

ThenĀ insertĀ theĀ cylindricalĀ partsĀ ofĀ theĀ Stepper Motor into the slots.

planktoscope-assembly-139.png

Feed the Stepper Motor cables intoĀ theĀ slotsĀ eitherĀ sideĀ ofĀ theĀ RaspberryĀ Pi Camera. !!! warning

Make sure the orientation isĀ correct and matches the picture.

ThenĀ insertĀ theĀ cylindricalĀ partsĀ ofĀ theĀ Stepper Motor into the slots.

!!! note

TheĀ resultĀ shouldĀ beĀ theĀ sameĀ asĀ theĀ picture.

Chapter 16: Connecting theĀ Raspberry Pi CameraĀ to theĀ Raspberry Pi HAT

planktoscope-assembly-141.png

  • We will need theĀ Raspberry Pi HAT with housing and Ribbon Cable alongĀ 
    with theĀ Raspberry Pi Camera withĀ Stepper Motors and housing.
  • We will be connecting theĀ šŸŸ¢RibbonĀ Cableto theĀ šŸŸ”blackĀ Raspberry Pi CameraĀ connectorĀ that we removedĀ it from earlier.

planktoscope-assembly-142.png

Gently feed theĀ Ribbon CableĀ into theĀ port of theĀ Camera.

!!! warning

šŸ‘ Ensure the correct orientation ofĀ theĀ Ribbon CableĀ with the blue endĀ facing upwards.

planktoscope-assembly-143.png šŸ”“Ā Press down on the blackĀ connector on the Raspberry PiĀ camera board once the RibbonĀ Cable is in position.

planktoscope-assembly-144.png šŸ”“Ā **B1.**Small flat screwdriver 2mm

planktoscope-assembly-145.png

  • Now we will plug in theĀ StepperĀ Mounts to theĀ HAT.
  • šŸ”“The cables for theĀ Stepper MountsĀ will be plugged into theĀ HATĀ šŸŸ£here.

!!! warning

Hold tight, a specific order isĀ required.

planktoscope-assembly-146.png

Starting with theĀ red cable, insert theĀ cable in the far left port and tightenĀ the screw situated above the port.

!!! note

The result should look the same asĀ the picture.

planktoscope-assembly-147.png

Repeat this process with the orderĀ pictured here from left to right:Ā šŸ”“ RedĀ šŸŸ” YellowĀ šŸ”µ BlueĀ āš« Black.

Chapter 17: Connect theĀ LEDĀ andĀ Peristaltic PumpĀ to theĀ Raspberry Pi

planktoscope-assembly-149.png

We will now be connecting the LEDĀ and Peristaltic Pump with theĀ Raspberry PiĀ HATĀ andĀ Camera.

planktoscope-assembly-150.png

Place the LED housing onto theĀ Stepper Mounts.

!!! warning

šŸ‘ Ensure correct orientation of bothĀ šŸŸ£ parts by looking at theĀ precut holes.

planktoscope-assembly-151.png

!!! info

The result should be similar to theĀ picture.

planktoscope-assembly-152.png

Now we will plug in theĀ LED andĀ Stepper MountĀ cables to theĀ Raspberry Pi.

planktoscope-assembly-153.png

Feed the area of cabling that is notĀ covered by theĀ spiral wrapĀ throughĀ the two holes to start. Then threadĀ through to theĀ spiral wrapĀ so that itĀ matches the picture.

planktoscope-assembly-154.png

We will now plug the cables into theĀ correct ports.

planktoscope-assembly-155.png

šŸŸ£Ā The four wires (šŸ”“ RedĀ šŸ”µ Blue šŸŸ¢ Green āš« Black) enter the side port on theĀ Raspberry Pi HAT.

planktoscope-assembly-156.png

šŸ”“ The two wires (LED) enter on theĀ port on top of the Raspberry Pi HAT.

planktoscope-assembly-157.png

The result should be similar to theĀ picture.

!!! note

We will use this assembly in the nextĀ step.

Chapter 18: Connect theĀ DC Power JackĀ to theĀ Raspberry Pi

planktoscope-assembly-159.png

  • šŸŸ¢Ā Locate theĀ DC Power Jack
  • We will plug the DC Power JackĀ into the Raspberry Pi via the šŸ”µ blue port.

planktoscope-assembly-160.png

šŸ”“Ā **B1.**Small flat screwdriver 2mm

planktoscope-assembly-161.png

  • Insert the šŸ”“ red cable of the DCĀ Power Jack into the left side of theĀ blue port.
  • Tighten the screw above.

planktoscope-assembly-162.png

  • Insert the āš« black cable of the DCĀ PowerĀ JackĀ intoĀ theĀ rightĀ sideĀ ofĀ theĀ blue port.
  • Tighten the screw above.

planktoscope-assembly-163.png

!!! info

The result should be similar to theĀ picture.

!!! note

We will use this assembly in the nextĀ step.

Chapter 19: Your PlanktoScope starts to take shape

planktoscope-assembly-165.png

planktoscope-assembly-166.png

Locate Part IĀ andĀ Part H.

planktoscope-assembly-167.png

  • SlotĀ Part HĀ intoĀ Part I.
  • šŸŸ£Ā Note the orientation.Ā Part HĀ goesĀ intoĀ Part Iat the end with theĀ rectangular slot (as opposed to theĀ rectangle with bulbous hole).
  • šŸŸ”Ā Also, the deeper slots onĀ part HĀ should be on the upper side.

planktoscope-assembly-168.png

šŸ”“ Slot theĀ Peristaltic PumpĀ aboveĀ theĀ LED.

planktoscope-assembly-169.png

!!! note

The result should be the same as theĀ picture.

planktoscope-assembly-170.png

!!! warning

šŸ‘ Ensure the correct orientation ofĀ the housing and theĀ **Peristaltic Pump**.

planktoscope-assembly-171.png

At the other end, slot theĀ DC PowerĀ JackĀ housing adjacent to theĀ Raspberry Pi.

planktoscope-assembly-172-00.png planktoscope-assembly-172-00.png

Rotate so that the DC Power Jack isĀ facing upwards. We will now slot the Raspberry Pi ontoĀ the rest of the housing.

planktoscope-assembly-173.png

!!! info

The result should be similar to thatĀ picture.

!!! note

šŸŽ¬Ā Store this assembly for later.

Chapter 20: Inserting screws

planktoscope-assembly-175.png

šŸŸ£ WeĀ willĀ nowĀ insertĀ eightĀ M3Ā screwsĀ to fasten the housing together.

!!! note

šŸŽ¬Ā Store this assembly for later.

planktoscope-assembly-176.png

šŸ”“Ā A6. Screw M3X12mm BHC - SS

planktoscope-assembly-177.png

šŸŸ”Ā B3. Allen key 2mm

planktoscope-assembly-178.png

!!! info

The result should be similar to theĀ picture.

planktoscope-assembly-179.png

We will now turn over theĀ PlanktoScopeĀ andĀ repeatĀ theĀ processĀ for the underside.

planktoscope-assembly-180.png

šŸŸ£Ā Insert eight more M3 screws on theĀ underside.

planktoscope-assembly-181.png

!!! info

The result should be similar to theĀ picture.

planktoscope-assembly-182.png

Now turn the PlanktoScope on its side.

planktoscope-assembly-183.png

planktoscope-assembly-184.png

!!! warning

šŸ‘ Ensure the orientation of yourĀ PlanktoScope andĀ Part KĀ matchesĀ the picture.

planktoscope-assembly-185.png

SlotĀ Part KĀ onto the rest of theĀ PlanktoScope.

!!! warning

šŸŸ¢Ā Ensure the correct orientation. TheĀ result should look the same at theĀ picture.

planktoscope-assembly-186.png

Content ofĀ Bag A: šŸ”“Ā A6. Screw M3X12mm BHC - SS

planktoscope-assembly-187.png

Content ofĀ Bag B: šŸŸ”Ā B3. Allen key 2mm

planktoscope-assembly-188.png

šŸŸ£Ā Insert eight more M3 screws on theĀ side to holdĀ Part KĀ in place.

planktoscope-assembly-189.png

planktoscope-assembly-190.png

We will now placeĀ Part JĀ into positionĀ as the housing for the side.

!!! warning

šŸ‘ Ensure your PlanktoScope matchesĀ the orientation in the picture.

planktoscope-assembly-191.png

šŸŸ¢ PlaceĀ Part KĀ onto the rest of theĀ PlanktoScope and note the positionĀ of the cutout.

planktoscope-assembly-192.png

Content ofĀ Bag A: šŸ”“Ā A6. Screw M3X12mm BHC - SS

planktoscope-assembly-193.png

Content ofĀ Bag B: šŸŸ”Ā B3. Allen key 2mm

planktoscope-assembly-194.png

šŸŸ£Ā Insert eight more M3 screws on theĀ underside

planktoscope-assembly-195.png

!!! info

The result should be similar to theĀ picture.

planktoscope-assembly-196.png

planktoscope-assembly-197.png

Content ofĀ Bag A: šŸŸ”Ā A5. Screw M2.5X10mm CHC - SS

planktoscope-assembly-198.png

PlaceĀ theĀ M2.5Ā screwĀ throughĀ PartĀ N. ItĀ willĀ actĀ asĀ aĀ coverĀ forĀ theĀ electricalĀ inputs.

planktoscope-assembly-199.png

  • Place theĀ coverĀ over the electricalĀ inputs on the PlanktoScope.
  • šŸŸ£ The screw will enter the holeĀ located here.

planktoscope-assembly-200.png

Using the allen key, tighten the screwĀ so that it is possible to move theĀ cover with light force.

planktoscope-assembly-201.png

!!! info

TheĀ resultĀ shouldĀ beĀ theĀ sameĀ asĀ theĀ picture.

Chapter 21: Insert the tubing in theĀ Peristaltic Pump

planktoscope-assembly-203.png

  • OrientateĀ yourĀ PlanktoScopeĀ soĀ thatĀ itĀ matches the picture.
  • Twist off the orange top of theĀ PeristalticĀ pumpĀ inĀ anĀ anti-clockwiseĀ direction.

planktoscope-assembly-205.png

You can now remove the PeristalticĀ Pump housing andĀ šŸŸ¢ Rotor.

planktoscope-assembly-206.png

You can now remove the PeristalticĀ Pump housing andĀ šŸŸ¢ Rotor.

planktoscope-assembly-207.png

šŸ”“ Locate theĀ TubeĀ for the PeristalticĀ Pump inĀ Bag FĀ and remove it from theĀ bag.

!!! warning

The tips of the Tubing that areĀ covered by black rubber are veryĀ delicate and easily broken.

planktoscope-assembly-208.png

Insert the first plastic arch of theĀ TubeĀ into the slot on the Peristaltic PumpĀ 
housing.

!!! info

The result should be similar to theĀ picture.

planktoscope-assembly-209-00.png planktoscope-assembly-209-01.png

Insert theĀ RotorĀ into the housingĀ ensuring the correct orientation. TheĀ hole in the centre should be visible onĀ the underside.

!!! info

The result should be similar to theĀ picture.

planktoscope-assembly-210.png

Insert theĀ RotorĀ into the housing.Ā Then, thread theĀ TubeĀ around theĀ RotorĀ and insert the other plasticĀ arch into the second slot.

!!! info

The result should be similar to theĀ picture.

planktoscope-assembly-211.png

  • Thread theĀ TubeĀ around around theĀ RotorĀ and insert the other plasticĀ arch into the second slot.
  • This will require stretching theĀ TubeĀ slightly.

planktoscope-assembly-212.png

!!! info

The result should be similar to theĀ picture.

planktoscope-assembly-213.png

Place the Peristaltic Pump housingĀ back onto the PlanktoScope.

planktoscope-assembly-214.png

Achieve the angle shown in theĀ picture between the Peristaltic PumpĀ housing and PlanktoScope mainĀ body. Then, press and twist in aĀ clockwise direction.

planktoscope-assembly-215.png

!!! info

The result should be similar to theĀ picture.

planktoscope-assembly-216.png

  • šŸŸ£Ā Gently remove the black rubberĀ covers for the Peristaltic PumpĀ connectors by pinching the very tipĀ and pulling away.
  • Once complete, locateĀ Bag DĀ whichĀ contains tubing.

planktoscope-assembly-217.png

Push the small piece of tubing fromĀ Bag DĀ over the left-side connector ofĀ theĀ Peristaltic Pump.

planktoscope-assembly-218.png

Place the long piece of tubing fromĀ Bag DĀ over the right-side connectorĀ of theĀ Peristaltic Pump.

planktoscope-assembly-219.png

Insert the connector fromĀ Bag DĀ intoĀ the other end of theĀ small piece ofĀ tubing.

planktoscope-assembly-220.png

planktoscope-assembly-221.png

OrientateĀ Part PĀ so that the magnetsĀ are face-down on the left-hand side.

planktoscope-assembly-222.png

Place Part P over the magnetsĀ adjacent to theĀ Peristaltic Pump.

!!! info

The result should be similar to theĀ picture.

planktoscope-assembly-223.png

šŸ”“ FromĀ Bag M, Place theĀ light blueĀ Test Tube in the hole adjacent to theĀ Peristaltic Pump.

planktoscope-assembly-224.png

Place theĀ šŸ”µ dark blue Test TubeĀ intoĀ the hole situated outside of theĀ PlanktoScope.

planktoscope-assembly-225.png

šŸŸ¢Ā Insert the other end of the longĀ piece of tubing into theĀ šŸ”µ dark blue Test Tube. This will serve as the waste container.Ā The result should look the same asĀ the picture.

planktoscope-assembly-226.png

planktoscope-assembly-227-00.png planktoscope-assembly-227-01.png planktoscope-assembly-227-02.png

  • Locate PartĀ C
  • LocateĀ 25Ā mmĀ cameraĀ lensĀ andĀ Lock RingĀ fromĀ Bag J.
  • 25MM is printed on the lens.

planktoscope-assembly-228-00.png planktoscope-assembly-228-01.png

  • Remove the plastic lens cap.
  • SlotĀ theĀ 25Ā mmĀ cameraĀ lens intoĀ Part C.

!!! info

The magnets are raised on theĀ side where the lens lays flat.

!!! warning

Try not to touch the lens

Screw theĀ Lock RingĀ onto the lens,Ā flat-side down.

planktoscope-assembly-229-00.png planktoscope-assembly-229-01.png planktoscope-assembly-227-02.png

  • Locate PartĀ D.
  • LocateĀ 16Ā mmĀ cameraĀ lensĀ andĀ LockĀ RingĀ fromĀ Bag J.
  • 16MM is printed on the lens.

planktoscope-assembly-230-00.png planktoscope-assembly-230-01.png

  • Remove the plastic lens cap.
  • SlotĀ theĀ 16Ā mmĀ cameraĀ lens into PartĀ D.

!!! info

TheĀ magnetsĀ areĀ indentedĀ onĀ theĀ side that the lens lays flat.

!!! warning

Try not to touch the lens

Screw theĀ Lock RingĀ onto the lens,Ā flat-side down.

The result should be similar to thatĀ picture.

planktoscope-assembly-231-00.png planktoscope-assembly-231-01.png

PlaceĀ bothĀ Lenses (CĀ andĀ D)Ā togetherĀ so that they flat together and bothĀ lenses are facing each other.

!!! info

The result should be similar to theĀ picture.

planktoscope-assembly-232-01.png planktoscope-assembly-232-00.png planktoscope-assembly-232-02.png

PlaceĀ theĀ LensesĀ (bothĀ CĀ andĀ D)Ā intoĀ position, adjacent to the camera.

The orientation of your PlanktoScopeĀ and Lenses should match theĀ pictures.

planktoscope-assembly-233.png

  • OnceĀ together,Ā placeĀ theĀ lensesĀ (bothĀ C and D) into position, adjacent toĀ the camera.
  • PartĀ CĀ /Ā 25mmĀ lensĀ shouldĀ beĀ furthestĀ from the pump (orange piece).

!!! info

The result should be similar to theĀ picture.

planktoscope-assembly-234.png

planktoscope-assembly-235.png

!!! info

The Fluidic Path will have aĀ cardboard protector.

Please take extra precaution while handlingĀ thisĀ partĀ andĀ avoidĀ touchingĀ the glass element of the piece.

!!! warning

The Fluidic Path is very delicate.

planktoscope-assembly-236.png

Place the Tube Clamp over the longĀ pieceĀ ofĀ tubeĀ atĀ theĀ endĀ ofĀ theĀ FluidicĀ Path.

planktoscope-assembly-237.png

Place the white clamp over the longĀ pieceĀ ofĀ tubeĀ atĀ theĀ endĀ ofĀ theĀ Fluidic Path.

!!! info

The result should look the same asĀ the picture.

planktoscope-assembly-238.png

Press down on the Tube Clamp soĀ that it clicks into place with just oneĀ click.

!!! info

The result should look the same asĀ the picture.

planktoscope-assembly-239.png

InsertĀ aĀ smallĀ plasticĀ ConnectorĀ intoĀ theĀ endĀ ofĀ theĀ tubing,Ā belowĀ theĀ TubeĀ Clamp.

planktoscope-assembly-240.png

Make sure the tubing is over theĀ Connector.

!!! info

The result should look the same asĀ the picture.

planktoscope-assembly-241.png

Remove the cardboard protectorĀ from theĀ Fluidic Path.

planktoscope-assembly-242.png

!!! warning

A reminder, the glass part of theĀ FluidicĀ PathĀ isĀ veryĀ delicate.Ā PleaseĀ tryĀ not to touch it.

planktoscope-assembly-243.png

Place another Connector at the endĀ of the tubing.

planktoscope-assembly-244.png

TheĀ resultĀ shouldĀ beĀ theĀ sameĀ asĀ theĀ picture.

planktoscope-assembly-245.png

āš  Gently place the Fluidic Path inĀ the designated indentation onĀ PartĀ F.

!!! warning

Do not touch the glass.Ā 
  • You may have to slightly stretch theĀ tubing to get the Fluidic Path intoĀ position.
  • TheĀ resultĀ shouldĀ beĀ theĀ sameĀ asĀ theĀ picture.

planktoscope-assembly-246.png

If you have tape available, place aĀ smallĀ pieceĀ atĀ theĀ theĀ bottomĀ ofĀ Part F so that the tubing remains fixed inĀ its position.

Avoid placing tape over the glass.

!!! info

The result should be similar to theĀ picture.

planktoscope-assembly-247-01.png planktoscope-assembly-247-00.png

ToĀ insertĀ theĀ syringe,Ā placeĀ fingerĀ andĀ thumb either side of part F whereĀ green circle is located. This ensuresĀ the tubing does not rotate while youĀ twist the syringe into position in aĀ clockwise direction.

planktoscope-assembly-248.png

!!! info

The result should be similar to theĀ picture.

planktoscope-assembly-249-00.png planktoscope-assembly-249-01.png

  • Place the Fluidic Path into positionĀ between the mount and theĀ Peristaltic Pump.
  • Connect the magnets to face eachĀ other.

planktoscope-assembly-250.png

The result should look the same asĀ the pictures.

planktoscope-assembly-251-00.png planktoscope-assembly-251-01.png

  • šŸŸ¢ Connect the Fluidic Path to theĀ Peristaltic Pump by twisting the twoĀ connectors together.
  • TheĀ resultĀ shouldĀ beĀ theĀ sameĀ asĀ theĀ picture.

Build complete! šŸ’Æ šŸ’«

The next stage is to plug in our PlanktoScopeĀ and connect it to the computer.