1.8 KiB
openSMAPPjs
My little SMAPP should be based on javascript and work without a backend server. The backend is only used to persist data and send it to the lab.
It should be possible to use the app as is - without the backend side.
For this data is stored in the local-storage of the browser.
Configure The App
- create the database
Sample Preparation Protocol
- settling time (default 15 s)
- dilution (main default 1:5 and bacterial default 1:500)
- sample Volume (1 mL)
- shaking time (30x shakes in 30s)
Microscope Camera Setup
- width / height of FoV [um]
- objective magnification (default 40x)
- eypiece Number
- size of slide width x height
Sample Intake Form
- type (compost)
- place
- date + time of taking the sample
- notes (when was the last rain event)
Sample Preparation
After the microscope and the preparation protocol is defined we can start taking in samples.
Sample Preparation
- dilution (main 1:5)
- shaking time (30x shakes in 30s)
- settling time (15 s)
Slide Preparation
- size of slide
- sample size [uL]
Sample Analysis
The analysis consists of a Nematode scan the main assessment for protozoa and filamentous organisms and a bacteria count.
Nematode Scan
Take multiple pictures of each Nematode and calssify to functional group. Bacterial- / Fungal- / Rootfeeding and Predatory Nematodes.
Main Assessment
Take multiple pictures per FoV and count all organisms in the FoV. Mark them and store the picture and list of organisms.
- as many as needed pictures per FoV.
- 5 or more FoV per reading.
- 5 or more readings.
Bacterial Count
For the bacterial count another dilution is used. Then bacteria are counted.
Results Page
From the different parts of the analysis the F:B ratio is calculated and Number of Protozoa / Fungal and Bacterial Biomass per mL of soil is calculated.